Biotechnology and Tissue culture
Mariem Lotfi; Chokri Bayoudh; Afifa Majdoub; Messaoud Mars
Abstract
Purpose: In Tunisia, pear cultivars are widely threatened by the attack of fire blight disease. Cultivation of tolerant cultivars is an effective control strategy for disease control. For this purpose, a reliable protocol was established for micropropagation of local Pyrus communis and Pyrus syriaca ...
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Purpose: In Tunisia, pear cultivars are widely threatened by the attack of fire blight disease. Cultivation of tolerant cultivars is an effective control strategy for disease control. For this purpose, a reliable protocol was established for micropropagation of local Pyrus communis and Pyrus syriaca L. and for large-scale production of high-quality plantlets. Research method: Using apical explants, different media and hormones were tested to establish a micropropagation procedure for local Tunisian Pyrus communis cultivars ‘Arbi’, ʻMaltiʼ, ʻMahdia 6ʼ and ʻMoknine 10ʼ and for Pyrus syriaca. Disinfection with 4% HgCl2 treatment for 20 minutes showed the highest percentage of plant survival. Successful initiation of the cultures was achieved on MS basal medium supplemented with 0.25 mg L-1 BA. Findings: During the proliferation stage, optimal shoot multiplication was obtained on MS medium with a half concentration of NH4NO3 and KNO3 supplemented with 0.1 mg L-1 IBA and 2 mg L-1 BA, but for maximum shoot length the BA concentration needed to be lowered to 1 mg L-1. A rooting rate of 100% and the highest root length and root number were attained on Cheng medium supplemented with 1.0 mg L-1 IBA. Pear vitroplants were successfully acclimatized on S2 substrate, composed by peat moss. Research limitations: Vitroplants acclimatization step needs to be well studied for the improvement of theacclimatized vitroplant survival rates by reducing the symptoms of crown rot. Originality/Value: This efficient optimized in vitro protocol will be successfully applied for large multiplication of high quality of Tunisian Pyrus vitroplants and cultivars.
Biotechnology and Tissue culture
Chadha Ayed; Chokri Bayoudh; Awatef Rhimi; Najla Mezghani; Faouzi Haouala; Bouthaina AL Mohandes Dridi
Abstract
Purpose: Tunisian garlic is widely threatened by the attack of several viruses genus. For this purpose, a reliable protocol was established for rapid in vitro propagation of local garlic (Allium sativum L.) cultivars for large-scale production of virus-free plants and high quality bulblets. ...
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Purpose: Tunisian garlic is widely threatened by the attack of several viruses genus. For this purpose, a reliable protocol was established for rapid in vitro propagation of local garlic (Allium sativum L.) cultivars for large-scale production of virus-free plants and high quality bulblets. Research method: Well disinfected shoot-tips of 1 mm were used as explants and cultivated on MS basal solid media enriched with various growth regulators: 6-Benzylaminopurine, α-Naphthaleneacetic acid, Kinetin, Indole-3-butyric acid and 2-isopentenyladenine for assessment of shoot formation, shoot proliferation and bulb formation. Findings: Among the different phytohormone concentrations and combinations, MS basal medium without any growth regulators (M0) was found optimal for shoot-tip initiation (96% explants development) and plantlets elongation (56.26 mm). For shoot proliferation, the M1 culture medium containing 1 mg L-1 BAP and 0.25 mg L-1 NAA was the best, giving a multiplication rate of 1.7 plantlets/explant. Shoots on M0 culture medium formed bulblets earlier. Multiple bulblets per explants were obtained on medium M22 containing 2 mg L-1 Kin and 0.1 mg L-1 NAA. Separated bulblets were transferred individually on bulbification media. Non-dividable bulblet was developed in various sizes. Research limitations: Bulblet acclimatization step needs to be well studied for high quality cloves production. Originality/value: This efficient optimized in vitro protocol will be successfully applied for large multiplication of virus-free garlic cultivars.
