Medicinal Plants
Tran Thien Hien; Tran Trong Kieu Ngoc; Nguyen Manh Ngoc Bao; Vo Trung Tinh; Pham Nguyen Thuy Dung
Abstract
Purpose: The study was conducted to optimize the extraction process with high polyphenol and flavonoid content from O. gratissimum L. and evaluate their biological activities. Research Method: The extraction of O. gratissimum L. extract was performed by combining ultrasound-assisted and hot extraction ...
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Purpose: The study was conducted to optimize the extraction process with high polyphenol and flavonoid content from O. gratissimum L. and evaluate their biological activities. Research Method: The extraction of O. gratissimum L. extract was performed by combining ultrasound-assisted and hot extraction methods. Findings: The results showed that extraction of O. gratissimum L. was performed under optimal conditions by response surface methodology (RSM), including ethanol concentration of 70%, raw material: ethanol ratio of 1: 35.71 (g.g-1), time extraction of 69.77 min, and at a temperature of 89.82 °C, with a total polyphenol content and a total flavonoid content of 190.987 (mgGAE.gDW-1) and 27.10 (mgQE.gDW-1), respectively. The extract was concentrated by vacuum rotary evaporation to obtain the total extract which was subjected to evaluation for its physicochemical components and biological activities. The results showed that the total extract quality met the current standards of Vietnam without heavy metals and pesticide residues. In addition, the antibacterial, antioxidant and anti-inflammatory properties of the total extract sample from O. gratissimum L. also gave positive results, confirming the significant biological activity. Research limitations: No limitations were encountered. Originality/Value: Ocimum gratissimum L. is a medicinal herb widely used in traditional medicine across East Asia, especially in Vietnam. Due to the high content of physiochemical components, including essential oils and phenolic compounds such as polyphenols and flavonoids, O. gratissimum L. exhibits antioxidant, anti-inflammatory, and antibacterial properties. Therefore, studies on the biological activity of solution extracts and total extracts from O. gratissimum L. are essential for evaluating the potential applications in the fields of medicine, foods, and cosmetics.
Medicinal Plants
Thi Kim Ngan Tran; Hoang Thien Vu Nguyen; Thi Thu Ha Nguyen; Thi Cam Thai
Abstract
Purpose: Study on the effects of extraction methods on the biological activities of Centella asiatica, including antibacterial and antioxidant properties. Research Method: The main components of C.asiatica include triterpenoid saponins, polyphenols, flavonoids, and other health-beneficial compounds found ...
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Purpose: Study on the effects of extraction methods on the biological activities of Centella asiatica, including antibacterial and antioxidant properties. Research Method: The main components of C.asiatica include triterpenoid saponins, polyphenols, flavonoids, and other health-beneficial compounds found through phytochemical screening. Ethanol extraction is performed using three extraction methods: immersion extraction, ultrasonic extraction, and reflux. Centella asiatica extract was tested for polyphenol content, total flavonoids, total triterpenoid saponins, and the ability to scavenge the free radicals DPPH and ABTS. Findings: The reflux extraction method was more effective than the other extraction methods in extracting chemical components, yielding relatively higher polyphenol, total flavonoid, and total triterpenoid saponins contents. All three types of extracts have the ability to fight oxidation, protecting cells from harmful free radicals. The IC50 value of pennywort extract in DPPH and ABTS free radical scavenging tests ranged from 455.52 and 270.05 µg/mL (soaked) < 333.63 and 206.56 µg/mL (ultrasound) < 239.75 and 199.75 µg/mL (reflux). The minimum inhibitory concentration (MIC) for the two bacterial strains Staphylococcus aureus and Escherichia coli was less than 2.188 mg/mL in all three methods. Research limitations: No limitations were encountered. Originality/Value: The development of extraction processes and evaluation of high quality extracts from gotu kola requires a combination of traditional methods and modern technology such as the use of advanced chemical and biological analytical techniques. This may create opportunities for the development of new technologies in the field of herbal extraction.
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