Medicinal Plants
Tran Thien Hien; Tran Trong Kieu Ngoc; Nguyen Manh Ngoc Bao; Vo Trung Tinh; Pham Nguyen Thuy Dung
Abstract
Purpose: The study was conducted to optimize the extraction process with high polyphenol and flavonoid content from O. gratissimum L. and evaluate their biological activities. Research Method: The extraction of O. gratissimum L. extract was performed by combining ultrasound-assisted and hot extraction ...
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Purpose: The study was conducted to optimize the extraction process with high polyphenol and flavonoid content from O. gratissimum L. and evaluate their biological activities. Research Method: The extraction of O. gratissimum L. extract was performed by combining ultrasound-assisted and hot extraction methods. Findings: The results showed that extraction of O. gratissimum L. was performed under optimal conditions by response surface methodology (RSM), including ethanol concentration of 70%, raw material: ethanol ratio of 1: 35.71 (g.g-1), time extraction of 69.77 min, and at a temperature of 89.82 °C, with a total polyphenol content and a total flavonoid content of 190.987 (mgGAE.gDW-1) and 27.10 (mgQE.gDW-1), respectively. The extract was concentrated by vacuum rotary evaporation to obtain the total extract which was subjected to evaluation for its physicochemical components and biological activities. The results showed that the total extract quality met the current standards of Vietnam without heavy metals and pesticide residues. In addition, the antibacterial, antioxidant and anti-inflammatory properties of the total extract sample from O. gratissimum L. also gave positive results, confirming the significant biological activity. Research limitations: No limitations were encountered. Originality/Value: Ocimum gratissimum L. is a medicinal herb widely used in traditional medicine across East Asia, especially in Vietnam. Due to the high content of physiochemical components, including essential oils and phenolic compounds such as polyphenols and flavonoids, O. gratissimum L. exhibits antioxidant, anti-inflammatory, and antibacterial properties. Therefore, studies on the biological activity of solution extracts and total extracts from O. gratissimum L. are essential for evaluating the potential applications in the fields of medicine, foods, and cosmetics.
Plant Breeding and Biotechnology
Sanam Safaei Chaeikar; Koorosh Falakro; Mehdi Rahimi; Shahin Jahangirzadeh khiavi; Masoumeh Ashourpour
Abstract
Purpose: Tea is one of the earliest caffeinated non-alcoholic drinks produced from the tip of young shoots. Evaluation of genetic diversity of clones that existed in tea germplasm can be a help to improve the tea breeding program. Research Method: The genetic diversity of 9 tea clones based on morphological, ...
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Purpose: Tea is one of the earliest caffeinated non-alcoholic drinks produced from the tip of young shoots. Evaluation of genetic diversity of clones that existed in tea germplasm can be a help to improve the tea breeding program. Research Method: The genetic diversity of 9 tea clones based on morphological, chemical and molecular markers were evaluated at Shahid Eftekhari Fashalam Experimental Station, Tea Research Center, Shaft, Guilan, Iran. Findings: Analysis of variance showed a significant difference between the clones for the studied traits. Descriptive statistics showed that green leaf yield had the highest phenotypic variation with CV of 56.47% and water extract showed the least phenotypic variation (4.40%). Clones 399, 285 and 100 had a significantly higher content of the number of plucking shoots, fresh and dry weight of plucking shoot and green leaf yield than other clones. Regarding the water extract, clones 272 and 100 have a significantly higher value than the other clones. Contents of polyphenols in all of clones were high except clones 276 and 278. The cluster analysis classified tea clones into three groups based on morphological and chemical traits as well as SCoT markers. Research limitations: Not using other molecular markers and biochemical traits. Originality/Value: Great variation of morphological characters was apparent among the selected clones. Based on the Mantel test, the grouping of clones with molecular data was partially corresponding with morphological and chemical traits.
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