Biotechnology and Tissue culture
Maryam Dehestani-Ardakani; Mohsen Karimi Dorche; Maryam Rahmati
Abstract
Purpose: Caladium bicolor is highly valued as both a landscape and indoor plant, primarily for its decorative appeal stemming from its diverse leaf shapes and vibrant, multicolored foliage. LED (light-emitting diode) lighting serves as a cost-efficient and potent means of promoting plant growth and development. ...
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Purpose: Caladium bicolor is highly valued as both a landscape and indoor plant, primarily for its decorative appeal stemming from its diverse leaf shapes and vibrant, multicolored foliage. LED (light-emitting diode) lighting serves as a cost-efficient and potent means of promoting plant growth and development. The impact of different LED lights was investigated on callus induction, regeneration, and plantlet growth of two cultivars of Caladium bicolor (‘White’ and ‘Red’). Research Method: Leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 1.5 mg L-1 IBA and 1 mg L-1 BA and moved to racks equipped with various LED lighting (100% red lights (R), 100% blue lights (B), 50% blue + 50% red lights (B+R), and 100% white fluorescent lamps (W)). Findings: Results showed W light was the best for maximum callus induction, leaf number, and plantlet height in both cultivars. Red + blue LED light spectrum motivated proliferation percentage of callus in both cultivars as compared to other light spectra. Conservation of ‘White’ caladium plantlets in R and B light spectra resulted in no hyperhydric micro shoot formation incidences. When examining various growth characteristics, it was evident that the B+R light spectrum of ‘Red’ caladium showed the best performance, while the B light spectrum in both cultivars had the least favorable outcomes compared to all other light spectra. Research limitations: There was no limitation. Originality/Value: Our findings offer a deeper understanding of how the quality of LED light impacts the in vitro propagation of caladium, potentially enhancing the cultivation of these plantlets through specific spectral exposure.
Biotechnology and Tissue culture
Soghra Rezaie; Maryam Dehestani-Ardakani; Kazem Kamali
Abstract
Purpose: Due to pharmaceutical value of Stevia plant (Stevia rebudiana Bertoni.), this study was done to introduce a new protocol for rapid mass propagation of itthrough tissue culture. Research Method: In MS medium shoot proliferation of stevia by six concentrations of BA (0, 0.1, 0.2, 0.3, 0.4, 0.5 ...
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Purpose: Due to pharmaceutical value of Stevia plant (Stevia rebudiana Bertoni.), this study was done to introduce a new protocol for rapid mass propagation of itthrough tissue culture. Research Method: In MS medium shoot proliferation of stevia by six concentrations of BA (0, 0.1, 0.2, 0.3, 0.4, 0.5 mg·l-1) and root induction by four concentrations of IBA (0, 0.025, 0.05, 0.1 mg·l-1) was investigated.Rooting of cuttings was done both in vitro and ex vivo conditions. Findings: According to the results, the most number of stems obtained in MS medium containing 0.3 mg l-1 BA. The highest length of stems obtained in MS medium without BA and the most number of leaves observed in MS medium supplemented with 0.4 mg·l-1 BA. In in vitro situation, the most number and length of roots obtained in MS medium without IBA. The most number of rooted cuttings was obtained in IBA solution after 72 hours and about 70% of rooted cuttings were healthy. Research limitations: It had no limitation to report. Originality/value: In conclusion, it seems that the potential of producing root and shoots in stevia plant is extremely high, so its proliferation is possible using low concentrations of plant growth regulators in in vitro culture.
