Document Type: Original Article


1 Department of Horticultural Science, University of Zanjan, Zanjan, Iran

2 Official agent of Iran Agricultural Insurance-Guilan

3 Tea Research Center, Horticultural Science Research Institute, AREEO, Lahijan- Iran


Purpose: The use of natural and organic products increases to produce a healthy product free of pollutants. Tea seeds contain 15-20% oil, which used as edible oil and an organic pesticide. In previous years, these seeds were used to produce tea seedlings. But now, cuttings are used for tea propagation and seeds are a byproduct of tea production. Therefore, this study aims to compare the effect of TSO with commercial wax on post-harvest quality of Citrus sinensis cv. Moro. Research method: An experiment was done as Completely Randomized Design; fruits were coated with control (distilled water), commercial wax (XEDASOL- MX20), and tea seed oil. The fruits were stored for 60 days in cold storage. The weight of the fruit skin, the contents of juice, total soluble solids, titratable acidity, and anthocyanins of the fruit were measured. Findings: The results showed that fruits coated with commercial wax and tea seed oil have the lowest fruit weight loss, the highest percentage of TSS (10.42%), the most top content of anthocyanins (15.17 mg/l) and flavonoids (196.17 mg/l) that there was a significant difference with control. The lowest titratable acidity (2.28%) was observed in fruits impregnated with tea seed oil, and there was a considerable difference with control. In general, the effect of tea seed oil was similar to commercial wax. Limitations: No limitations were founded. Originality/Value: These results indicate that the application of tea seed oil proved to be effective in extending the quality and storage time of Citrus sinensis (cv. Moro).


Main Subjects

Aboutalebi, A., & Janparvar, F. (2010). The impact of Peppermint and Eucalyptus extract on reducing waste after harvesting Orange Washington Noll. 5th National Conference on New Ideas in Agriculture. Islamic Azad University, Khorasgan branch (Isfahan).

Ahmad, M., Abubakar, M. M., & Sale, S. (2020). Enhancing the shelf life of tomato fruits using plant material during storage. Journal of Horticulture and Postharvest Research, 3(2), 347-354.

Ahmed, F. F., Kamel, M. K., & Ibrahim, H. I. (2014). The synergistic effects of using plant extracts and salicylic acid on yield and fruit quality of keitte mango trees. Stem Cell, 5(2), 30-39.

Barzanooni, E., Aghkhani, M., Maskooki, A., & Abbaspourfard, M. (2014). Effect of pre-heat treatment and herbal essences on the postharvest properties of blood orange. Journal of Horticultural Science, 27(4), 418-423.

Bousbia, N., Vian, M. A., Ferhat, M. A., Meklati, B. Y., & Chemat, F. (2009). A new process for extraction of essential oil from Citrus peels: Microwave hydrodiffusion and gravity. Journal of Food Engineering, 90(3), 409-413. 

Cemeroglu, B. (1992). Basic analysis methods in fruit and vegetable processing industry. BILTA University Book Series, 02-2.

Chen, S., & He, T. Y. (2005). A review on refinement of tea seed oil and its application. Journal of Chemical Industry of Forest Products (Bimonthly), (6), 8.

Chen, W., Zhang, Z., Shen, Y., Duan, X., & Jiang, Y. (2014). Effect of tea polyphenols on lipid peroxidation and antioxidant activity of litchi (Litchi chinensis Sonn.) fruit during cold storage. Molecules, 19(10), 16837-16850.

Dela, G., Or, E., Ovadia, R., Nissim-Levi, A., Weiss, D., & Oren-Shamir, M. (2003). Changes in anthocyanin concentration and composition in ‘Jaguar’rose flowers due to transient high-temperature conditions. Plant Science, 164(3), 333-340. (02)00417-X

El-Eleryan, E. E. (2015). Effect of chitosan and green tea on the quality of Washington Navel orange during cold storage. American Journal of Plant Physiology, 10(1) 43-54. /10.3923/ajpp.2015.43.54 

Fallico, B., Lanza, M. C., Maccarone, E., Asmundo, C. N., & Rapisarda, P. (1996). Role of hydroxycinnamic acids and vinylphenols in the flavor alteration of blood orange juices. Journal of Agricultural and Food Chemistry, 44(9), 2654-2657. /10.1021/jf9503319

Fazel, M., Sahari, M. A., & Barzegar, M. (2008). Determination of main tea seed oil antioxidants and their effects on common kilka oil. International Food Research Journal, 15, 209-217.

Giusti, M. M., & Wrolstad, R. E. (2001). Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy. Current Protocols in Food Analytical Chemistry, 00(1), F1.2.1-F1.2.13. https://doi:10.1002/0471142913.faf0102s00

Graham, H. N. (1992). Green tea composition, consumption, and polyphenol chemistry. Preventive Medicine, 21(3), 334-350. /10.1016/0091-7435(92)90041-f 

Hamedani, M. A. H. S. A., Rabiei, V. A. L. I., Moradi, H. O. S. S. E. I. N., & Ghanbari, A. (2014). Effect of temperature and storage period on biochemical changes and postharvest quality of Moro blood orange fruit (Citrus sinensis cv. Moro). Iranian Journal of Horticultural Science, 44(4), 367-377. / ijhs.2013.50361

Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacology and Therapeutics, 96(2-3), 67-202. /10.1016/s0163-7258(02)00298-x 

He, L., Guo-ying, Z., Huai-yun, Z., & Jun-ang, L. (2011). Research progress on the health function of tea oil. Journal of Medicinal Plants Research, 5(4), 485-489. /10.5897/jmpr11.1344 

Kerio, L. C., Wachira, F. N., Wanyoko, J. K., & Rotich, M. K. (2012). Characterization of anthocyanins in Kenyan teas: Extraction and identification. Food Chemistry, 131(1), 31-38. /10.1016/j.foodchem.2011.08.005 

Khatiwora, E., Adsul, V. B., Torane, R. C., Gaikwad, S., Deshpande, N. R., & Kashalkar, R. V. (2017). Spectroscopic determination of total phenol and flavonoid contents of citrus limon peel from north eastern region of India. Journal of Drug Delivery and Therapeutics, 7(1). /10.22270/jddt.v7i1.1368

Klimczak, I., Małecka, M., Szlachta, M., & Gliszczyńska-Świgło, A. (2007). Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. Journal of Food Composition and Analysis, 20(3-4), 313-322. /10.1016/j.jfca.2006.02.012

Lee, W. T., Tung, Y. T., Wu, C. C., Tu, P. S., & Yen, G. C. (2018). Camellia oil (Camellia oleifera Abel.) modifies the composition of gut microbiota and alleviates acetic acid-induced colitis in rats. Journal of Agricultural and Food Chemistry, 66(28), 7384-7392. /10.1021/acs.jafc.8b02166 

Liu, L., Cao, S., Xie, B., Sun, Z., & Wu, J. (2007). Degradation of cyanidin 3-Rutinoside in the presence of (–)-epicatechin and Litchi Pericarp polyphenol oxidase. Journal of Agricultural and Food Chemistry, 55(22), 9074-9078. /10.1021/jf071931y 

Nor-Natur APS. 2003. Trial with Quiponin BS products in a Danish vineyard. 1-6. Nor-Natur APS, Copenhagen, Denmark.

Nor-Natur APS. 2004. Quiponin: Plant growth stimulant in strawberries. Nor-Natur APS. 1-3. Nor-Natur APS, Copenhagen, Denmark.

Oh, Y. S., Lee, J. H., Yoon, S. H., Oh, C. H., Choi, D. S., Choe, E., & Jung, M. Y. (2008). Characterization and quantification of anthocyanins in grape juices obtained from the grapes cultivated in Korea by HPLC/DAD, HPLC/MS, and HPLC/MS/MS. Journal of Food Science, 73(5), C378-C389. /10.1111/j.1750-3841.2008.00756.x 

Okey, N.E. (2015). Biocontrol of post-harvest fungal diseases of Citrus scinensis (Sweet orange) using leaf extracts of Azadirachta indica (neem) and Chromolaena odorata. Journal of Plant and Agriculture Research, 1(1), 1-8.

Rapisarda, P., Bianco, M. L., Pannuzzo, P., & Timpanaro, N. (2008). Effect of cold storage on vitamin C, phenolics and antioxidant activity of five orange genotypes [Citrus sinensis (L.) Osbeck]. Postharvest Biology and Technology, 49(3), 348-354. /10.1016/j.postharvbio.2008.02.002 

Sahari, M. A., Ataii, D., & Hamedi, M. (2004). Characteristics of tea seed oil in comparison with sunflower and olive oils and its effect as a natural antioxidant. Journal of the American Oil Chemists' Society, 81, 585-588. /10.1007/s11746-006-0945-0 

Scalzo, R. L., Iannoccari, T., Summa, C., Morelli, R., & Rapisarda, P. (2004). Effect of thermal treatments on antioxidant and antiradical activity of blood orange juice. Food Chemistry, 85(1), 41-47. /10.1016/j.foodchem.2003.05.005 

Shoja, A., GhasemNejad, M., & Mortazavi, N. (2011). Changes in antioxidant capacity and quality after harvest of Thomson and blood orange juice during storage. Journal of Horticultural Science, 25(2), 147-155.

Thumula, P. (2006). Studies on storage behavior of tomatoes coated with chitosan-lysozyme films. Doctoral dissertation, McGill University.

Tolly, W., Rahemi, M., & Karimi, Z. 2007, Investigation of the effects of Thyme and Ginger oil extracts on orange mold fruit orange fruit in Anbar. 5th Horticultural Sciences Congress. Shiraz University.

Tsuda, T. (2012). Dietary anthocyanins rich plants: Biochemical basis and recent progress in health benefits studies. Molecular Nutrition and Food Research, 56(1), 159-170. /10.1002/mnfr.201100526 

Turhan, K. N. (2009, April). Is edible coating an alternative to MAP for fresh and minimally processed fruits? In X International Controlled and Modified Atmosphere Research Conference 876 (pp. 299-305). /10.17660/actahortic.2010.876.40 

Yang, X., Wang, X., Wang, K., Su, L., Li, H., Li, R., & Shen, Q. (2015). The nematicidal effect of Camellia seed cake on root-knot nematode Meloidogyne javanica of banana. PloS one, 10(4), e0119700. /10.1371/journal.pone.0119700

Zaki, Z. A., Yousef, A. R. M., Abd El-Moneim, E. A. A., & Emam, H. E. (2017). Effect of some natural extracts on maintaining quality of zaghloul date palm fruits during cold storage. Middle East Journal of Agriculture Research, 6(2), 464-473.

Zhang, Z., Hu, M., Yun, Z., Wang, J., Feng, G., Gao, Z., & Jiang, Y. (2017). Effect of tea seed oil treatment on browning of litchi fruit in relation to energy status and metabolism. Postharvest Biology and Technology, 132, 97-104. /10.1016/j.postharvbio.2017.05.010 

Zhao, B., Li, X., He, R., Cheng, S., & Wenjuan, X. (1989). Scavenging effect of extracts of green tea and natural antioxidants on active oxygen radicals. Cell Biophysics, 14(2), 175-185. /10.1007/bf02797132