Document Type: Original Article

Authors

1 Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Horticulture, Abhar Branch, Islamic Azad University, Abhar, Iran

Abstract

Purpose: Micropropagation of GF677 rootstock, the most widely clonal rootstock used in peach orchards, is an important method for large-scale production of disease-free plants. In this research, effects of media, plant growth regulators and carbohydrates in order to optimize the efficient micropropagation protocol of GF677 rootstock has been investigated. Research method: In vitro shoots were multiplicated on MS, WPM and DKW media supplemented with 0, 0.5, 1, 2 mg L-1 BA. Proliferated shoots were rooted on MS, WPM and DKW media containing 0, 0.5, 1, 2 mg L-1 IBA. In another experiment, the effect of carbohydrate type was investigated. Findings: High shoot number and node number as well as shoot fresh weight were achieved with shoot tips when cultured on MS medium supplemented with 1 or 2 mg L-1 BA. The highest percentage of rooted shoots was obtained on MS or WPM media supplemented with 1 or 2 mg L-1 IBA. Maximum root number was regenerated on WPM medium containing 1 mg L-1 IBA. Sorbitol was found to be more effective carbon source on shoot multiplication than sucrose, while the highest average of root number and root length were observed in the medium containing sorbitol and sucrose medium, respectively. Survival rate during the acclimatization in the greenhouse was 67%. Limitations: Plant acclimatization needs to be studied for commercial production. Originality/Value: This protocol has proven useful for micropropagation of GF677 rootstock.

Graphical Abstract

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Aftabi, M., Mozaffari, J., Hossein Ava, S., & Miri, S.M. (2013). Selection an appropriate medium for in vitro proliferation of hazelnut genotypes. 8th National Horticultural Science Congress of Iran. Hamedan, Iran (pp. 2628-2631). (in Farsi).

Ahmad, T., Abbasi, N.A., Hafiz, I.A., & Ali, A. (2007). Comparison of sucrose and sorbitol as main carbon energy sources in micropropagation of peach rootstock GF-677. Pakistan Journal of Botany, 39(4), 1269-1275.

Arab, M.M., Yadollahi, A., Eftekhari, M. Ahmadi, H., Akbari, M. & Sarikhani Khorami, S. (2018). Modeling and optimizing a new culture medium for in vitro rooting of G×N15 Prunus rootstock using artificial neural network-genetic algorithm. Scientific Reports, 8, 9977. https://doi.org/10.1038/s41598-018-27858-4

Arab, M.M., Yadollahi, A., Shojaeiyan, A., Shokri, S., & Maleki Ghojah, S. (2014). Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock. Journal of Genetic Engineering and Biotechnology, 12(2), 81-87. https://doi.org/10.1016/j.jgeb.2014.10.001

Bagheri, S., Davoodi, D., Amiri, M.E., Bayanati, M., & Entesari, M. (2017). Effect of different culture media on the micropropagation of GF677 (Prunus amygdalus × P. persica). Journal of Horticultural Science, 30(4), 616-623. (in Farsi)

Balla, I., & Mansvelt, L. (2012). Micropropagation of peach rootstocks and cultivars. In M. Lambardi, E. Ozudogru, & S. Jain (Eds.). Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology (Methods and Protocols), 994. Totowa, Humana Press. https://doi.org/10.1007/978-1-62703-074-8_10

Bell, R.L., Srinivasan, C., & Lomberk, D. (2009). Effect of nutrient media on axillary shoot proliferation and preconditioning for adventitious shoot regeneration of pears. In Vitro Cellular & Developmental Biology – Plant, 45, 708. https://doi.org/10.1007/s11627-009-9196-8

Byrne, D.H., Raseira, M.B., Bassi, D., Piagnani, M.C., Gasic, K., Reighard, G.L., Moreno, M.A., & Pérez, S. (2012). Peach. In M.L. Badenes, & D.H. Byrne (Eds.). Fruit Breeding, Handbook of Plant Breeding. Springer Science + Business Media.

Davoudabadi Farahanie, F., Miri, S.M., & Nabigol, A. (2020). Optimization of in vitro propagation of Paulownia tomentosa. Iranian Journal of Plant & Biotechnology, 15(1), 1-9. (in Farsi)

De Paiva Neto, V.B., & Otoni, W.C. (2003). Carbon sources and their osmotic potential in plant tissue culture: does it matter? Scientia Horticulturae, 97(3-4), 193-202. https://doi.org/10.1016/S0304-4238(02)00231-5

Driver, J.A., & Kuniyuki, A.H. (1984). In vitro propagation of Paradox walnut rootstock. HortScience, 19(4), 507-509.

Erfani, M., Miri, S.M., & Imani, A. (2017). In vitro shoot proliferation and rooting of Garnem rootstock as influenced by basal media, plant growth regulators and carbon sources. Plant Cell Biotechnology and Molecular Biology, 18(3&4), 101-109.

Fallahpour, M., Miri, S.M., & Bouzari, N. (2015). In vitro propagation of Gisela 5 rootstock as affected by media and plant growth regulators. Journal of Horticultural Research, 23(1), 57-64.

Fallahpour, M., Miri, S.M., & Bouzari, N. (2019). Effects of media cultures and plant growth regulators on micropropagation of CAB-6P cherry semi-dwarf rootstock. Iranian Journal of Horticultural Science, 50(1), 187-196. (in Farsi)

Felipe, A.J., Gómez-Aparisi, J., Socías, R., & Carrera, M. (1997). The almond × peach hybrid rootstocks breeding program at Zaragoza (Spain). Acta Horticulturae, 451, 259-262.     https://doi.org/10.17660/ActaHortic.1997.451.28.

Gainza, F., Opazo, I., Guajardo, V., Meza, P., Ortiz, M., Pinochet, J., & Muñoz, C. (2015). Rootstock breeding in Prunus species: Ongoing efforts and new challenges. Chilean journal of agricultural research, 75(Supl. 1), 6-16. https://dx.doi.org/10.4067/S0718-58392015000300002.

George, E.F., Hall, M.A., & Klerk, G.J.D. (2008). The Components of plant tissue culture media II: Organic additions, osmotic and pH effects, and support systems. In E.F. George, M.A. Hall, & G.J.D. Klerk (Eds.). Plant Propagation by Tissue Culture. Dordrecht, Springer (pp. 115-173).

Guajardo, V., Hinrichsen, P., & Muñoz, C. (2015). Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model. Chilean Journal of Agricultural Research, 75(Suppl. 1), 17-27. https://dx.doi.org/10.4067/S0718-58392015000300003

Güney, M., Çömlekçioğlu, S., Kefeyati, S., Kafkas, E., & Kafkas, S. (2016). In vitro mass propagation of GF677 rootstock. Journal of Atatürk Horticultural Central Research Institute, 45, 867-870.

Hartmann, H.T., Kester, D.E., Davies, F.T., Geneve, R.L., & Wilson, S.B. (2020). Hartmann & Kester's Plant Propagation: Principles and Practices. USA: Pearson.

Hasanloo, T., Jafarkhani Kermani, M., Malmir Chegini, M., Sepehrifar, R., Mohajeri Naraghi, S., & Miri, S.M. (2014). Optimization of in vitro propagation of Qare-Qat (Vaccinium arctostaphylus). Journal of Medicinal Plants and By-products, 2, 21-26.

Kamali, K., Majidi, E., & Zarghami, R. (2001). Determination of the most suitable culture medium and growth conditions for micropropagation of GF677 (hybrid of almond × peach) rootstocks. Seed and Plant, 17(3), 234-243. (in Farsi)

Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia by use shoot-tip culture. International Plant Propagators' Society, 30, 421-427.

Legua, P., Pinochet, J., Moreno, M.Á., Martínez, J.J., & Hernández, F. (2012). Prunus hybrids rootstocks for flat peach. Scientia Agricola, 69(1), 13-18. https://doi.org/10.1590/S0103-90162012000100003

Marino, G., Bertazza, G., Magnanini, E., & Altan A.D. (1993). Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot. Plant Cell, Tissue and Organ Culture, 34, 235-244. https://doi.org/10.1007/BF00029712

Mayer, N.A., Bianchi, V.J., Feldberg, N.P., & Morini, S. (2017). Advances in peach, nectarine and plum propagation. Revista Brasileira de Fruticultura, 39(4), 355. https://doi.org/10.1590/0100-29452017355

Mestre, L., Reig, G., Betrán, J.A., Pinochet, J., & Moreno, M.Á., (2015). Influence of peach–almond hybrids and plum-based rootstocks on mineral nutrition and yield characteristics of ‘Big Top’ nectarine in replant and heavy-calcareous soil conditions. Scientia Horticulturae, 192, 475-481.      https://doi.org/10.1016/j.scienta.2015.05.020

Miri, S.M. (2017). Effect of IAA, IBA and NAA auxins on in vitro rooting of M.9 and M.26 apple rootstocks. Cellular and Molecular Plant Biology Journal, 12(4), 15-23. (in Farsi).

Miri, S.M., & Roughani, A. (2018a). Factors affecting tissue culture success in ornamental crops, I. medium composition. In 2nd International and 3rd National Congress on Flower and Ornamental Plants, Mahallat, Iran.

Miri, S.M., & Roughani, A. (2018b). Factors affecting tissue culture success in ornamental crops, II. genotype, explant and physical environment. 2nd International and 3rd National Congress on Flower and Ornamental Plants, Mahallat, Iran.

Miri, S.M., Vaez Livari, B., Khalighi, A., & Ghaemmaghami, S.A. (2003a). Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9 apple rootstock. Pajouhesh-va-Sazandegi, 59, 31-37. (in Farsi)

Miri, S.M., Vaez Livari, B., Khalighi, A., & Ghaemmaghami, S.A. (2003b). Phenolic oxidation reduction and in vitro proliferation of shoots of apple clones ‘M.9’ and ‘M.26’. Iranian Journal of Horticultural Science and Technology, 4(3 & 4), 145-154. (in Farsi).

Mousavi, S.S., S Miri, S.M., & Moradi, P. (2017). Optimization of micropropagation of jujube (Ziziphus jujuba cv. Tian-yuzao). Journal of Agronomy and Plant Breeding, 13(4), 1-11. (in Farsi).

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x.

Nazary Moghaddam Aghaye, R., & Yadollahi, A. (2012). Micropropagation of GF 677 Rootstock. Journal of Agricultural Science, 4(5), 131-138. http://dx.doi.org/10.5539/jas.v4n5p131.

Özden, A.N., Ak, B.E., & Özden, M. (2011). In vitro regeneration and micropropagation of GF-677 hybrid (Prunus amygdalus × P. persica) rootstock. Acta Horticulturae, 912, 653-656.    https://doi.org/10.17660/ActaHortic.2011.912.98

Rogalski, M., Moraes, L.K.A., Feslibino, C., Crestani, L., Guerra, M.P., & Silva, A.L. (2003). In vitro rooting of Prunus rootstocks. Revista Brasileira de Fruticultura, 25(2), 293-296. https://doi.org/10.1590/S0100-29452003000200028

Rubio-Cabetas, J.M. (2016). Almond Rootstocks: Overview. In O. Kodad, A. López-Francos, M. Rovira, & R. Socias i Company. XVI GREMPA Meeting on Almonds and Pistachios. Zaragoza, Spain (pp. 133-143).

Sepahvand, S., Ebadi, A., Kamali, K., & Ghaemmaghami, S.A. (2012). Effects of myo-inositol and thiamine on micropropagation of GF677 (peach × almond hybrid). Journal of Agricultural Science, 4(2), 275-280. http://dx.doi.org/10.5539/jas.v4n2p275.

Tatari, M., & Mousavi, S.A. (2014). Optimization of in vitro culture in Tetra, Nemaguard and GF677 clonal rootstocks. Journal of Crops Improvement, 15(3), 103-115. https://doi.org/10.22059/JCI.2013.36389. (in Farsi).

Tsafouros, A., & Roussos, P.A. (2018). First report of Krymsk® 5 (cv. VSL 2) cherry rootstock in vitro propagation: Studying the effect of cytokinins, auxins and endogenous sugars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(1), 152-161. https://doi.org/10.15835/nbha47111276

Yaseen, M., Ahmad, T., Sablok, G., Standardi, A., & Ahmad Hafiz, I. (2013). Review: role of carbon sources for in vitro plant growth and development. Molecular Biology Reports, 40, 2837-2849. https://doi.org/10.1007/s11033-012-2299-z.