Document Type: Original Article


1 High Agronomic Institute, 4042 Chatt-Mariem, IRESA-University of Sousse, Tunisia.

2 National Gene Bank of Tunisia


Purpose: Despite the significance of garlic as a food product and high annual income, until now there are no local commercialized Tunisian cultivars with registered names, which lead to the lack of recommended varieties of high yielding. This work is aimed to create the first garlic gene bank collection in Tunisia, evaluate the variation, identify yield related traits and structure genetic diversity among them. Research method: Thirty six local garlic landraces from the main production regions of Tunisia were collected and recorded in the National Gene Bank of Tunisia database, then grown in a gene bank field for multiplication. The phenotypic diversity was conducted on the basis of fourteen quantitative characteristics add to flowering ability. Findings: High diversity among Tunisian garlic landraces was detected. The bulb weight and bulb diameter explain the most significant variation of the yield. The cluster analysis sorted the 36 genotypes into three main groups as cluster 1 (11 accessions), cluster 2 (20 accessions), and cluster 3 (5 accessions). However, the grouping of genotypes did not correspond with their geographic origin. The highest genetic distance was reported between NGBTUN442 and NGBTUN452, however NGBTUN429 and NGBTUN434 found to be most similar with the lowest dissimilarity. Research limitation: Morphological traits need to be investigated overtime. Originality/Value: This study is interesting since it presents the first creation of the garlic gene bank in Tunisia. The results will provide basis information for the efficient use of the local garlic germplasm and help breeders to easily select out the desirable materials.


Main Subjects

Abdel-Rasheed, K.G., & Moustafa, Y.M.M. (2016). Efficiency of genotype selection for yield potentiality in garlic under organic agriculture conditions. Egyptian Journal of Plant Breeding, 20(3), 465-92.

Al Gehani, I., & Kanbar, A. (2013). Multivariate statistical analysis of bulb yield and morphological characters in garlic (Allium sativum L.). Australian Journal of Basic and Applied Sciences, 7(14), 353-358.

Ayed, C., Chokri, B., Awatef, R., Najla, M., Faouzi, H., & Bouthaina, A.D. (2018). In vitro propagation of tunisian local garlic (Allium sativum L.) from shoot tip culture. Journal of Horticulture and Postharvest Research, 1 (2), 75-86.

Baghalian, K., Naghavi, M. R., Ziai, S.A., & Badi, H.N. (2006). Post-planting evaluation of morphological characters and allicin content in Iranian garlic (Allium sativum L.) ecotypes. Scientia Horticulturae, 107 (4), 405-10.

Baghalian, K., Sanei, M.R., Naghavi, M.R., Khalighi, A., & Naghgi Badi, H.A. (2005). Post culture evaluation of morphological divergence in Iranian garlic ecotypes. Acta Horticulturae, 688, 123-125.

Benke, A.P., Dukare, S., Mahajan, V., & Singh, M. (2018). Genetic divergence studies for bulbing and related traits in garlic germplasm during kharif season. International Journal of Current Microbiology and Applied Sciences, 7 (1), 2920-27.

Etoh, T.. & Simon, P.W. (2002). Diversity, fertility and seed production of garlic. In Allium crop science: Recent Advances (Rabinowitch, H.D., & Currah, L. (Eds.)). CAB Int. Wallingford, 101-117.

Fritsch, R.M., & Friesen, N. (2002). Evolution, domestication and taxonomy. In Allium crop science: recent advances, (Ed.). Rabinowitch, H. D., & Currah, L., Wallingford: CABI, 5-30.

IPGRI. International Plant Genetic Resources Institute. (2001). Descriptors of Allium Allium Spp. Rome.

Jabbes, N., Arnault, I., Auger, J., Al Mohandes Dridi, B., & Hannachi, C. (2012). Agro-morphological markers and organo-sulphur compounds to assess diversity in Tunisian garlic landraces. Scientia Horticulturae, 148 (December), 47–54.

Jabbes, N., Geoffriau, E., Le Clerc, V., Dridi, B., & Hannechi, C. (2011). Inter simple sequence repeat fingerprints for assess genetic diversity of Tunisian garlic populations. Journal of Agricultural Science, 3 (4).

Kasso, M., & Balakrishnan, M. (2013). Ex situ conservation of biodiversity with particular emphasis to Ethiopia. ISRN Biodiversity, 1–11.

Kaushik, S., Kumar, M., Prakash, S., Kumar, V., Kumar Singh, M., Singh, B.,  Malik, S., & Singh, K. (2016). Study of genetic diversity in garlic (Allium sativum L.) by using morphological characters. Progressive Agriculture, 16(2), 204.

Panse, R., Jain, P.K., Gupta, A., & Sasode, D.S. (2013). Morphological variability and character association in diverse collection of garlic germplasm. African Journal of Agricultural Research, 8 (23), 2861-2869.

Panthee, D.R., Kc, R.B., Regmi, H.N.,  Subedi, P.P., Bhattarai, S., & Dhakal, J. (2006). Diversity analysis of garlic (Allium sativum L.) germplasms available in Nepal based on morphological characters. Genetic Resources and Crop Evolution, 53 (1), 205–12.

Petropoulos, S., Fernandes, A., Ntatsi, G., Petrotos, K., Barros, L., & Ferreira, I. (2018). Nutritional value, chemical characterization and bulb morphology of Greek garlic landraces. Molecules, 23 (2), 319.

Sabir, M., Singh, D., & Jat, B. L. (2017). Study of morphological and molecular characterization of garlic (Allium sativum L.). The Asian Journal of Horticulture, 12, 141–159.

Silva, A.R., Cecon, P.R., Dias, C.T.S., Puiatti, M., Finger, F.L., & Carneiro, A.P.S. (2014). Morphological phenotypic dispersion of garlic cultivars by cluster analysis and multidimensional scaling. Scientia Agricola, 71(1), 38-43.

Stavělíková, H. (2008). Morphological characteristics of garlic (Allium sativum L.) genetic resources collection-information. Horticultural Science, 35(3), 130-135.

UPOV. Union for the protection of new varieties of plants. (2001). Guidelines for  the conduct of tests for distinctness, uniformity and stability. Garlic (Allium sativum L.), Geneva.

Volk, G.M., & Stern, D. (2009). Phenotypic characteristics of ten garlic cultivars grown at different North American locations. HortScience, 44(5), 1238-1247.

Wang, H., Li, X., Shen, D., Oiu, Y., & Song, J. (2014). Diversity evaluation of morphological traits and allicin content in garlic (Allium sativum L.) from China. Euphytica, 198(2), 243-54.

Yeshiwas, Y., Belete, N., Tegibew, W., Yohaness, G., Abayneh, M., & Kassahun, Y. (2018). Collection and characterization of garlic (Allium sativm L.) germplasm for growth and bulb yield at Debre Markos, Ethiopia. Journal of Horticulture and Forestry, 10(3), 17-26.