Biotechnology and Tissue culture
Naser Askari
Abstract
Purpose: Lily is one of the most economically ornamental plants and tissue culture plays a vital role in accelerating mass propagation of lily. In lily tissue culture, the production of bigger bulblets is highly desirable. The objective of the present investigation is to examine the impact of gibberellin ...
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Purpose: Lily is one of the most economically ornamental plants and tissue culture plays a vital role in accelerating mass propagation of lily. In lily tissue culture, the production of bigger bulblets is highly desirable. The objective of the present investigation is to examine the impact of gibberellin on the in vitro growth of lily bulblets, administered at two distinct time intervals. Research method: In the present investigation, various concentrations of gibberellins (0, 0.1, and 1 µM) were employed at two distinct time points: the commencement of the culture period and the fifth week of culture period. After 11 weeks the fresh weight of bulblets, the number of bulblets, the fresh weight of leaves, the fresh weight of roots and the fresh weight of scale explant were scored and analyzed. Findings: The application of 1 µM gibberellin during bulblet induction yielded noteworthy outcomes, including a substantial 91% increase in the fresh weight of the bulblets, a significant 38% augmentation in the fresh weight of the leaves, as well as a 40% increase in the fresh weight of the roots. Research limitations: The quantification of endogenous phytohormones in lily scale explants was deemed unfeasible. Originality/value: The development of lily bulblets experienced a notable enhancement while the medium was supplemented with gibberellin in bulblet induction stage.
Plant Nutrition
Sally Wilkinson; Anna Weston; David Marks
Abstract
Purpose: Supplying plants with nitrogen in ammonium nitrate- or urea-based fertiliser is wasteful: much is degraded before acquisition, releasing environmental pollutants. Preventing urea degradation can reduce pollution and improve crop nitrogen use efficiency. We investigate benefits to ureic stabilisation, ...
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Purpose: Supplying plants with nitrogen in ammonium nitrate- or urea-based fertiliser is wasteful: much is degraded before acquisition, releasing environmental pollutants. Preventing urea degradation can reduce pollution and improve crop nitrogen use efficiency. We investigate benefits to ureic stabilisation, on flowering and stress tolerance, as organic nitrogen sources favourably alter biomass partitioning in this regard. Research Method: We test effects of adding chemically stabilised urea to soil, on the physical form and flowering of containerised, greenhouse-grown pelargonium, petunia, pansy and marigold, when transplanting seedlings to larger pots. Efficacies of stabilised urea, non-stabilised urea and industry standard fertiliser are compared under identical total nitrogen supply. The significance of treatment differences is calculated using a one-tailed t-test. Findings: Development is favourably altered by ureic stabilisation. Earliest changes measured are increased root lengths, leaf growth rates and chlorophyll concentrations. Plants then develop more shoots and 25-130% more flowers. Improvements arise partially through increased nitrogen longevity in soil, and partially through positive effects of urea itself on biomass partitioning between organs, and on plant physiology; giving rise to improved commercial attributes (more branches and flowers) and tolerance to stress (more root, less apical dominance, more chlorophyll). Research Limitations: Further research could measure leachate nitrogen content, and compare different methods of ureic stabilisation in more crops. Originality/Value: Urea stabilisation can increase fruit and flower yields, whilst reducing vulnerability to erratic climates, and fertiliser-derived pollution. We propose that urea’s effectiveness arises because plants have evolved strategies to proliferate whilst competing with micro-organisms for organic nitrogen.
