Document Type : Short Communication Article


1 Department of Horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Department of Food Biotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran.


Purpose: Saffron (Crocus sativus L.) is a valuable medicinal plant with a short flowering period. Its flowering management can be useful for the production of saffron in a farm or controlled condition. The objective of this study was to determine the effect of plant growth regulators (PGRs) on saffron corms sprouting. Research method: For this purpose, corms were treated with different PGRs including α-naphthaleneacetic acid (NAA) (0, 100, 200, 300 ppm), chlorocholine chloride (CCC) (0, 100, 200, 300 ppm) and gibberellic acid (GA3) (0, 5, 10, 20 ppm) for two hours and incubated at 20-22 °C for eight weeks. Findings: The results revealed that the effect of PGRs on corms sprouting was significant. It has been shown that treatment of corms with auxin at all concentrations reduced sprouting compared to the control. To prolong dormancy for five weeks, NAA at 200 mg L-1 was the best choice. While for the fifth week onwards, the most effective treatment was NAA at 100 ppm. Treatment with GA3 (20 ppm) has shown a stimulatory effect on corm sprouting. Research limitations: No limitations were founded. Originality/Value: Sprouting acceleration can keep flowering away from early autumn frosting in farm conditions, while prolongation of corms dormancy provides the possibility of harvesting saffron flowers in several times in a hydroponic system. The results of this study suggested two kinds of chemical for different purposes, inhibition and stimulation of sprouting of saffron corms that can be applicable for saffron hydroponic or farm production, respectively.


Main Subjects

Aytekin, A. & Acikgoz, A. O. (2008). Hormone and microorganism treatments in the cultivation of saffron (Crocus Sativus L.) plants. Molecules, 13, 1135-1146. https://doi.10.3390/molecules13051135
Chao, W. S., Serpe, M. D., Anderson, J. V., Gesch, R. W., & Horvath, D. P. (2006). Sugars, hormones, and environment affect the dormancy status in underground adventitious buds of leafy spurge (Euphorbia esula). Weed Science, 54(1), 59-68.
Christie, E. (2014). Water and nutrient reuse within closed hydroponic systems. Georgia Southern University. Electronic Theses and Dissertations. Under the Direction of George Fu and Doug Aubrey.
Girardin, O., Nindjin, C., Farah, Z., Escher, F., Stamp, P., & Otokore, D. (1998). Use of gibberellic acid to prolong dormancy and reduce losses during traditional storage of yams. Journal of the Science of Food and Agriculture, 77(2), 172-178.;2-r
Hayden, A. L. (2006). Aeroponic and hydroponic systems for medicinal herb, rhizome, and root crops. Horticultural Science, 41(3), 536-538.
Hoseinifard, M. S., Ghorbani Javid, M., Allahdadi, I. & Soltani, E. (2017). The Effect of hormone priming and corm weight on the yield of flowers and characteristics of daughter corms of saffron in the first year. Saffron Agronomy & Technology, 6(1), 3-15.
Koocheki, A. & Seyyedi, S. M. (2015). Phonological stages and formation of replacement corms of saffron (Crocus sativus L.) during growing period. Journal of saffron research, 3(2), 134-154.
Koocheki, A., Nassiri, M., Alizadeh, A., & Ganjeali, A. (2010). Modelling the impact of climate change on flowering behaviour of saffron (Crocus sativus L.). Journal of Agricultural Research, 7(2), 583-94.
Langens-Gerrits, M. M., Miller, W. B., Croes, A. F., & De Klerk, G. J. (2003). Effect of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Plant growth regulation, 40(3), 267-275.
Liu, X., Zhang, H., Zhao, Y., Feng, Zh., Li, Q., Yang. H. Q., Luan, Sh., Li, H., & He, Z. H. (2013). Auxin control seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis. Plant Biology, 110(38), 15485-90.
Maggio, A., Raimondi, G., Martino, A., & De Pascale, S. (2006, October). Soilless cultivation of saffron in Mediterranean environment. In III International Symposium on Models for Plant Growth, Environmental Control and Farm Management in Protected Cultivation 718. Wageningen, Netherlands (pp. 515-522).
Mojtahedi, N., Koobaz, P., Fathi, M., Dabirashrafi, O., Azadi, P., & Khosravi, S. (2014). Maturating, enlarging and breaking dormancy of in vitro Lilium bulblets. International Journal of Horticulture Science and Technology, 1(2), 101-9.
Okagami, N., & Tanno, N. (1993). Gibberellic acid-induced prolongation of the dormancy in tubers or rhizomes of several species of East Asian Dioscorea. Plant Growth Regulation, 12(1-2), 119-123.
Padmalatha, T., Reddy, G. S., Chandrasekhar, R., Shankar, A. S., & Chaturvedi, A. (2013). Effect of pre-planting soaking of corms with chemicals and plant growth regulators on dormancy breaking and corm and cormel production in gladiolus. International Journal of Plant, Animal and Environmental Sciences, 3(1), 28-33.
Sajjad, Y., Jaskani, M. J., Qasim, M., Mehmood, A., Ahmad, N., & Akhtar, G. (2015). Pre-plant soaking of corms in growth regulators influences the multiple sprouting floral and corm associated traits in Gladiolus grandiflorus L. Journal of Agricultural Science, 7(9), 173-81.
Sharma, K, Lee, Y. R., Parka, S. W. & Nile, H. (2016). Importance of growth hormones and temperature for physiological regulation of dormancy and sprouting in onions. Food Reviews International, 32(3), 233-55.
Thomas, J. E., & Riker, A. J. (1945). Sprouting of potatoes inhibited by plant hormones. The American Potato Journal, 22(4), 104-13.
Tschannen, A. B., Girardin, O., Nindjin, C., Daouda, D., Farah, Z., Stamp, P., & Escher, F. (2003). Improving the application of gibberellic acid to prolong dormancy of yam tubers (Dioscorea spp). Journal of the Science of Food and Agriculture, 83(8), 787-796.
Turhan, H., Kahriman. F., Egesel, C. O., & M. K. (2007). The effects of different growing media on flowering and corm formation of saffron (Crocus sativus L). African Journal of Biotechnology, 6(20), 2328-32.
Wang, H. Q. & Xiao, L. T. (2009). Effect of chlorocholine chloride on phytohormones and photosynthetic characteristics in potato (Solanum tuberosum L.) Plant Growth Regulator, 28(1), 21-7.
Zheng, R., Wu, Y., & Xia, Y. (2012). Chlorocholine chloride and paclobutrazol treatment promote carbohydrate accumulation in bulbs on Lilium oriental hybrids ‘Sorbonne. Journal of Biomedicine and Biotechnology, 13(2), 136-44.