An optimized protocol for in vitro propagation of Pyrus communis and Pyrus syriaca using apical-bud microcuttings

Lotfi, Mariem; Bayoudh, Chokri; Majdoub, Afifa; Mars, Messaoud

doi:10.22077/jhpr.2019.2420.1055

Document Type : Original Article

Authors

¹ Research Unit on Agrobiodiversity (UR13AGR05), Department of Horticultural Sciences, Higher Agronomic Institute, IRESA-University of Sousse, 4042 Chott-Mariem, Sousse, Tunisia.

² Regional Research Centre on Horticulture and Organic Agriculture (CRRHAB)

https://doi.org/10.22077/jhpr.2019.2420.1055

Abstract

Purpose: In Tunisia, pear cultivars are widely threatened by the attack of fire blight disease. Cultivation of tolerant cultivars is an effective control strategy for disease control. For this purpose, a reliable protocol was established for micropropagation of local Pyrus communis and Pyrus syriaca L. and for large-scale production of high-quality plantlets. Research method: Using apical explants, different media and hormones were tested to establish a micropropagation procedure for local Tunisian Pyrus communis cultivars ‘Arbi’, ʻMaltiʼ, ʻMahdia 6ʼ and ʻMoknine 10ʼ and for Pyrus syriaca. Disinfection with 4% HgCl₂treatment for 20 minutes showed the highest percentage of plant survival. Successful initiation of the cultures was achieved on MS basal medium supplemented with 0.25 mg L^-1 BA. Findings: During the proliferation stage, optimal shoot multiplication was obtained on MS medium with a half concentration of NH₄NO₃ and KNO₃supplemented with 0.1 mg L^-1IBA and 2 mg L^-1 BA, but for maximum shoot length the BA concentration needed to be lowered to 1 mg L^-1. A rooting rate of 100% and the highest root length and root number were attained on Cheng medium supplemented with 1.0 mg L^-1IBA. Pear vitroplants were successfully acclimatized on S₂ substrate, composed by peat moss. Research limitations: Vitroplants acclimatization step needs to be well studied for the improvement of theacclimatized vitroplant survival rates by reducing the symptoms of crown rot. Originality/Value: This efficient optimized in vitro protocol will be successfully applied for large multiplication of high quality of Tunisian Pyrus vitroplants and cultivars.

Keywords

Main Subjects

Biotechnology and Tissue culture

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An optimized protocol for in vitro propagation of Pyrus communis and Pyrus syriaca using apical-bud microcuttings

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References

Volume 3, Issue 1 - Serial Number 6March 2020March 2020Pages 1-10

Volume 3, Issue 1 - Serial Number 6
March 2020
March 2020
Pages 1-10