Identification of genetic diversity and relationships of some Iranian tea genotypes using SRAP markers

Jahangirzadeh, Shahin; Azadi Gonbad, Reza; Falakro, Koorosh

doi:10.22077/jhpr.2019.2582.1067

Document Type : Original Article

Authors

¹ Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran

² Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

https://doi.org/10.22077/jhpr.2019.2582.1067

Abstract

Purpose: The tea plant is one of the most important products in the northern region of Iran, and plays an essential role in the region's economy. Since today many tea plants in the region are being destroyed for various reasons, so having information about the genetics of those trees helps design breeding programs to reach appropriate plants for specific purposes. Research Method: SRAP markers, using eight primer combinations, were used to study the genetic relationships of 27 tea plant samples. Findings: In total, these eight combinations produced 41 scorable bands, 70.63% of which were polymorphic. The calculated PIC for all combinations was from 0.23 to 0.43 at an average of 0.36. Data analysis was performed by NTSYS software using Jaccard's similarity coefficient to determine the amount of similarity and the dendrogram was drawn based on UPGMA. Based on molecular data, the range of similarity between samples varied from 0.393 to 0.933. Samples were divided into five groups at a similarity level of 0.65. The fifth group (E) was divided into four subgroups at a similarity level of 0.75. Research limitations: Application of another marker system such as SSR and AFLP can help to understand the relationships of samples better. Originality/Value: In general, the study of genetic diversity showed that the SRAP marker could be useful in identifying polymorphic regions and estimating genetic distances and germplasm management in tea plants.

Keywords

Main Subjects

Plant Breeding and Biotechnology

References

Aggarwal, R. K., Brar, D. S., Nandi, S., Huang, N., & Khush, G. S. (1999). Phylogenetic relationships among Oryza species revealed by AFLP markers. Theoretical and Applied Genetics, 98(8), 1320-1328. https://doi.org/10.1007/s001220051198

Ben-Ying, L. I. U., You-Yong, L. I., Yi-Chun, T. A. N. G., Li-Yuan, W. A. N. G., Cheng, H., & Ping-Sheng, W. A. N. G. (2010). Assessment of genetic diversity and relationship of tea germplasm in Yunnan as revealed by ISSR markers. Acta Agronomica Sinica, 36(3), 391-400. https://doi.org/10.1016/S1875-2780(09)60037-7

Culley, T. M., & Wolfe, A. D. (2001). Population genetic structure of the cleistogamous plant species Viola pubescens Aiton (Violaceae), as indicated by allozyme and ISSR molecular markers. Heredity, 86(5), 545. https://doi.org/10.1046/j.1365-2540.2001.00875.x

Dellaporta, S. L., Wood, J. & Hicks, J. B. (1983). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1(4), pp.19-21. https://doi.org/10.1007/BF02712670

Fang, D. Q., Roose, M. L., Krueger, R. R., & Federici, C. T. (1997). Fingerprinting trifoliate orange germplasm accessions with isozymes, RFLPs, and inter-simple sequence repeat markers. Theoretical and Applied Genetics, 95(1-2), 211-219. https://doi.org/10.1007/s001220050550

Ferriol, M., Pico, B. & Nuez, F. (2003). Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics, 107(2), 271-282. https://doi.org/10.1007/s00122-003-1242-z

Jahangirzadeh Khiavi, Sh. & Falakro. K. (2017, February), Investigation of Genetic Diversity between Tea Shrubs Based on ISSR Markers, In: Proceedings of first national conference of Ecology, Diversity and Plant Conservation, Iran, Tehran (in Farsi).

Jahangirzadeh Khiavi, Sh., Falakro. K., Chaichi siahkali, H. & Keshavarzi, Sh. (2016, December). Study of Diversity between Tea Genotypes in Lahijan Region, In: Proceedings of third international and sixth national conference of medical herbs and stable agriculture, Iran, Hamedan (in Farsi).

Khadivi-Khub, A., Jahangirzadeh, S., Ahadi, E. & Aliyoun, S. (2014). Nuclear and chloroplast DNA variability and phylogeny of Iranian apples (Malus domestica). Plant Systematics and Evolution, 300(8), 1803-1817. https://doi.org/10.1007/s00606-014-1007-y

Khiavi, S. J., & Ashourpour, M. (2017). Characterization of the genetic relationships among some of Iranian apple genotypes using RAPD markers. Agricultural Communications, 5(2), 1-7.

Khiavi, S. J., Hamidoghli, Y., Golein, B., & Sabouri, A. (2016). Assessment of lime genetic diversity in three regions of Iran using morphological and ISSR markers. Agricultural Communications, 4(3), 18-29.

Khiavi, S.J., Hamidoghli, Y., Golein, B. & Sabouri, A., (2015). Evaluation of genetic diversity in acid lime ('Citrus aurantifolia'swingle) genotypes using AFLP markers. Australian Journal of Crop Science, 9(10), 996-1002.

Koopman, W. J., Wissemann, V., De Cock, K., Van Huylenbroeck, J., De Riek, J., Sabatino, G. J., & Werlemark, G. (2008). AFLP markers as a tool to reconstruct complex relationships: a case study in Rosa (Rosaceae). American Journal of Botany, 95(3), 353-366. https://doi.org/10.3732/ajb.95.3.353

Li, G., & Quiros, C.F. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103, 455-461. https://doi.org/10.1007/s001220100570

Li, H. L. (1983). The domestication of plants in China: ecogeographical considerations. In: The Origins of Chinese Civilization. (Ed). University of California Press, USA, 21-64.

Li, P., Zhan, X., Que, Q., Qu, W., Liu, M., Ouyang, K., Li, J., Deng, X., Zhang, J., Liao, B. & Pian, R., (2015). Genetic diversity and population structure of Toona Ciliata Roem. based on sequence-related amplified polymorphism (SRAP) markers. Forests, 6(4), 1094-1106. https://doi.org/10.3390/f6041094

Li, Y., Fan, X., Shi, T., Zhang, Q., & Zhang, Z. (2009). SRAP marker reveals genetic diversity in tartary buckwheat in China. Frontiers of Agriculture in China, 3(4), 383.

Lin, P., Yao, X. H., Wang, K. L., Zheng, T. T., & Teng, J. H. (2010). Identification and genetic analysis of Camellia oleifera changlin series superior clones by SRAP molecular marker. Journal of Agriculture Biotechnology, 18, 272-279.

Liu, L. J., PENG, D. X. & Bo W.A. N. (2008). Genetic relation analysis on ramie (Boehmeria nivea (L.) Gaud.) inbred lines by SRAP markers. Agricultural Sciences in China, 7, 944-949. https://doi.org/10.1016/S1671-2927(08)60133-9

Lu, Y. & Foo, L. Y. (2002). Polyphenolics of Salvia – a review. Phytochemistry, 59(2), 117-140.

Mondal T. K., Satya, P. & Medda, P. S. (2003, November). India needs national tea germplasm repository.In International Conference on Global Advances in Tea Science, India, Calcutta, 58-59.

Pang, X. M., Hu, C. G., & Deng, X. X. (2007). Phylogenetic relationships within Citrus and its related genera as inferred from AFLP markers. Genetic Resources and Crop Evolution, 54(2), 429-436. https://doi.org/10.1007/s10722-006-0005-5

Peng, F., Wu, Y., Hao, M., Chen, L., & Chen, Y. (2012). Genetic diversity of Camellia oleifera using ISSR and SRAP markers. Journal of Nanjing Forestry University (Natural Sciences Edition), 36(5), 19-25.

Purseglove, J.W. (1968). Tropical crops: dicotyledons. London, Longmans Green.

Rincon, F., Johnson, B., Crossa, J. & Taba, S. (1996), Cluster analysis, an approach to sampling variability in maize accessions, Maydica, 41, 307-316.

Roldain-Ruiz, I., Calsyn, E., Gilliand, T. J., Coll, R., Van Eijk, M. J. T., & De Loose, M. (2000). Estimating genetic conformity between related ryegrass (Lolium) varieties, 2. AFLP characterization. Molecular Breeding, 6, 593-602. https://doi.org/10.1023/A:1011398124933

Saebnazar, A., & Rahmani, F. (2013). Genetic Variation Among Salvia Species Based on Sequence-Related Amplified Polymorphism (SRAP) Marker. Journal of Plant Physiology & Breeding, 3(1), 71-78.

Seanz-Romero, C., Guzman-Reyan, R. & Rehfeldt, G. E. (2006). Altitudinal genetic variation among Pinus oocarpa population in Michoacan, Mexico Inpications for seed zoning, conservation, tree breeding and globak warming. Forest Ecology and Management, 229, 340-350.

Simmons, M. P., Zhang, L. B., Webb, C. T., & Müller, K. (2007). A penalty of using anonymous dominant markers (AFLPs, ISSRs, and RAPDs) for phylogenetic inference. Molecular Phylogenetics and Evolution, 42 (2), 528-542. https://doi.org/10.1016/j.ympev.2006.08.008

Wang, Y., Sun, X., Tan, B., Zhang, B., Xu, L.A., Huang, M., & Wang, M. (2010). A genetic linkage map of Populus adenopoda Maxim.× P. alba L. hybrid based on SSR and SRAP markers. Euphytica, 173(2). 193-205. https://doi.org/10.1007/s10681-009-0085-3

Wickremaratne, M. R. (1981). Variation in some leaf characteristics in tea (Camellia sinensis L.) and their use in the identification of clones. Tea Q. 50(4), 183-189.

Wight, W. (1959). Nomenclature and classification of tea plant. Nature. 183, 1726-1728. https://doi.org/10.1038/1831726a0

Wolfe, A. D., & Elisens, W. J. (1993). Diploid hybrid speciation in Penstemon (Scrophulariaceae) revisited. American Journal of Botany, 80(9), 1082-1094. https://doi.org/10.2307/2445754

Yong, H. (2013). Analysis of Camellia meiocarpa genetic diversity based on SRAP markers. Scientia Silvae Sinicae, 49 (3), 43-50.

Journal of Horticulture and Postharvest Research

Identification of genetic diversity and relationships of some Iranian tea genotypes using SRAP markers

References

References

Volume 3, Issue 1 - Serial Number 6
March 2020
March 2020
Pages 25-34

Identification of genetic diversity and relationships of some Iranian tea genotypes using SRAP markers

References

References

Volume 3, Issue 1 - Serial Number 6March 2020March 2020Pages 25-34

Volume 3, Issue 1 - Serial Number 6
March 2020
March 2020
Pages 25-34