Document Type : Original Article

Authors

Department of Horticultural Science, University of Birjand, Birjand, Iran

Abstract

Purpose: The effect of foliar application of gibberellic acid and boric acid on bioactive compounds and quality of pomegranate fruit cv. Shishe-Kab was investigated. Research Method: This was accomplished through the field and laboratory experiments at the Faculty of Agriculture, University of Birjand, Iran, during 2017 and 2018 seasons. Gibberellic acid (50 and 150 mg L-1) and Boric acid (200 and 600 mg L-1) were applied three times at one-month interval; late in May, June, and July during growth and developmental stages of fruits. The experiment was set in a completely randomized block design. Fruits were harvested at full maturity stage late in October, and physicochemical properties were evaluated. Findings: Results showed that anthocyanin content and antioxidant activity significantly decreased while fruit firmness, ascorbic acid content, and fruit cracking increased in gibberellic acid treatments. Application of 50 mg L-1 gibberellic acid significantly increased total soluble solids content and maturity index, but decreased titratable acidity in the fruits.However, foliar application of gibberellic acid and boric acid did not have significant effects on the pH and phenolic compounds of the fruit. Research limitations: Inability to examine different pomegranate cultivars in the investigation was a limitation. Originality/Value: Pre-harvest foliar use of gibberellic acid and boric acid showed positive and negative effects on postharvest quality attributes of Shishe-Kab pomegranate. Thus, further studies need for practical application.

Keywords

Main Subjects

Ahmed, F., Mohamed, M., Abou El-Khashab, A., & Aeed, S. (2014). Controlling fruit splitting and improving productivity of Manfalouty pomegranate trees by using salicylic acid and some nutrients. World Rural Observations, 6(1), 87-93.
Arora, R., & Singh, S. (2015). Effect of growth regulators on quality of ber (Zizyphus mauritiana Lamk) cv. Umran. Agricultural Science Digest, 34(2), 102-106. https://doi.org/10.5958/0976-0547.2014.00024.X
Arzani, K., Khoshghalb, H., Malakouti, M. J., & Barzegar, M. (2008). Postharvest fruit physicochemical changes and properties of Asian (Pyrus serotina Rehd.) and European (Pyrus communis L.) pear cultivars. Horticulture Environment and Biotechnology, 49(1), 244-252.
Asgharzade, A., Valizade, G. A., & Babaeian, M. (2012). Investigating the effect of boron spray on yield nutrient content, texture and brix index of apple (Sheikh Amir Variety) in Shirvan region. African Journal of Microbiology Research, 6(11), 2682-2685. https://doi.org/10.5897/AJMR11.1163
Belsare, C. R. (2011). Effect of plant bio-regulators and nutrients on yield, fruit cracking and quality in pomegranate (Punica granatum L.) cv. G-137. (Doctoral dissertation, UHF, Nauni, Solan).
Bhoyar, M. G., & Ramdevputra, M. V. (2016). Effect of foliar spray of zinc, iron and boron on the growth, yield and sensory characters of guava (Psidium guajava L.) Cv. Sardar L-49. Journal of Applied and Natural Science, 8(2), 701-704. https://doi.org/10.31018/jans.v8i2.860
Buchert, J., Koponen, J. M., Suutarinen, M., Mustranta, A., Lille, M., Törrönen, R., & Poutanen, K. (2005). Effect of enzyme‐aided pressing on anthocyanin yield and profiles in bilberry and blackcurrant juices. Journal of the Science of Food and Agriculture, 85(15), 2548-2556. https://doi.org/10.1002/jsfa.2284
Cakmak, I., & Römheld, V. (1997). Boron deficiency-induced impairments of cellular functions in plants. Plant and Soil, 193(1-2), 71-83. https://doi.org/10.1023/A:1004259808322
Christensen, J. V. (1972). Cracking in cherries: IV. Physiological studies of the mechanism of cracking. Acta Agriculturae Scandinavica, 22(3), 153-162. https://doi.org/10.1080/00015127209433474
Chuah, A. M., Lee, Y.-C., Yamaguchi, T., Takamura, H., Yin, L.-J., & Matoba, T. (2008). Effect of cooking on the antioxidant properties of coloured peppers. Food Chemistry, 111(1), 20-28. https://doi.org/10.1016/j.foodchem.2008.03.022
Cline, J., & Trought, M. (2007). Effect of gibberellic acid on fruit cracking and quality of Bing and Sam sweet cherries. Canadian Journal of Plant Science, 87(3), 545-550. https://doi.org/10.4141/P06-132
Cochran, R., Adams, D., Wallace, J. & Galyean, M. (1986). Predicting digestibility of different diets with internal markers: evaluation of four potential markers. Journal of Animal Science, 63(5), 1476-1483. https://doi.org/10.2527/jas1986.6351476x 
Davarpanah, S., Tehranifar, A., Davarynejad, G., Abadía, J., & Khorasani, R. (2016). Effects of foliar applications of zinc and boron nano-fertilizers on pomegranate (Punica granatum cv. Ardestani) fruit yield and quality. Scientia Horticulturae, 210, 57-64. https://doi.org/10.1016/j.scienta.2016.07.003
Dhineshkumar, V., Ramasamy, D., & Srivastav, P, P. (2015). Modified atmosphere packaging of pomegranate arils: Review. International Journal of Applied Engineering and Technology ISSN. Vol. 5 (3), pp.8-24.
Ergüneş, G., & Tarhan, S. (2006). Color retention of red peppers by chemical pretreatments during greenhouse and open sun drying. Journal of Food Engineering, 76(3), 446-452. https://doi.org/10.1016/j.jfoodeng.2005.05.046
Gil, M. I., Tomás-Barberán, F. A., Hess-Pierce, B., Holcroft, D. M., and Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48(10), 4581-4589. https://doi.org/10.1021/jf000404a
Glenn, G. M. (1989). Cuticular properties and postharvest calcium applications influence cracking of sweet cherries. Journal of the American Society for Horticultural Science, 114, 781-788.
Goldbach, H. E., Hartmann, D., & Rötzer, T. (1990). Boron is required for the stimulation of the ferricyanide‐induced proton release by auxins in suspension‐cultured cells of Daucus carota and Lycopersicon esculentum. Physiologia Plantarum, 80(1), 114-118. https://doi.org/10.1111/j.1399-3054.1990.tb04383.x
Halliwell, B. (2007). Biochemistry of oxidative stress. 35, 1147-1150. https://doi.org/10.1042/BST0351147
Harminder, K., Arvinder, S., Monika, G., & Randhawa, J. S. (2008). Effect of NAA and gibberellic acid on pre-harvest fruit drop and quality of Satluj Purple plum. Haryana Journal of Horticultural Sciences, 37(1/2), 31-32.
Hegazi, A., Samra, N., El-Baz, E., Khalil, B. M., & Gawish, M. (2014). Improving fruit quality of Manfalouty and Wonderfull pomegranates by using bagging and some spray treatments with gibberellic acid, calcium chloride and kaolin. Journal of Plant Production Mansoura University. 5(5), 779-792.
Jafari, S. 2014. Effect of growth regulators on seed firmness and some characteristics of pomegranate Yousef Khani cultivar. Master's Thesis. Faculty of Bu Ali Sina. Iran Hamedan. Iran.
Karami, K. 2016. Effects of gibberellic acid and zinc sulfate on control fruit cracking in pomegranate. Master's Thesis. Ilam University of Technology. Iran.
Khalaj, K., Ahmadi, N., & Souri M. K. (2015). Effect of calcium and boron foliar application on fruit quality in Asian pear cultivar ‘KS10’. Journal of Crop Production and Processing, 4 (14) :89-97
Khalil, H. A., & Aly, H. S. (2013). Cracking and fruit quality of pomegranate (Punica granatum L.) as affected by pre-harvest sprays of some growth regulators and mineral nutrients. Journal of Horticultural Science & Ornamental Plants, 5(2), 71-76.
Kishor, S., Maji, S., Meena, M., Deepa, H., Dwivedi, S. K., & Kumar, S. (2017). Effect of plant bio-regulators and chemicals on fruit physico-chemical traits of pomegranate (Punica granatum L.) cv. Bhagwa. Journal of Pharmacognosy and Phytochemistry, 6(4), 1573-1575.
Kulkarni, A. P., & Aradhya, S. M. (2005). Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93(2), 319-324. https://doi.org/10.1016/j.foodchem.2004.09.029
Kumar, K., Joon, M., Yadav, R., & Daulta, B. (2016). Effect of Growth Regulators and Micronutrients on Fruit Quality in Pomegranate. International Journal of Environment, Agriculture and Biotechnology, 1(4). https://doi.org/10.22161/ijeab/1.4.3
Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of ethnopharmacology, 109(2), 177-206. https://doi.org/10.1016/j.jep.2006.09.006
Laribi, A., Palou, L., Intrigliolo, D., Nortes, P., Rojas-Argudo, C., Taberner, V., Bartual, J., & Pérez-Gago, M. (2013). Effect of sustained and regulated deficit irrigation on fruit quality of pomegranate cv.‘Mollar de Elche’at harvest and during cold storage. Agricultural Water Management, 125, 61-70. https://doi.org/10.1016/j.agwat.2013.04.009
Lombardo, G., Cargnello, G., Bassi, M., Gerola, F., & Carraro, L. (1978). Pollen ultrastructure in different vine cultivars with low productivity. Vitis-Berichte ueber Rebenforschung mit Dokumentation der Weinbauforschung (Germany, FR).
O'Kelley, J. C. (1957). Boron effects on growth, oxygen uptake and sugar absorption by germinating pollen. American Journal of Botany, 44(3), 239-244. https://doi.org/10.1002/j.1537-2197.1957.tb08236.x
Ouzounidou, G., Ilias, I., Giannakoula, A., & Papadopoulou, P. (2010). Comparative study on the effects of various plant growth regulators on growth, quality and physiology of Capsicum annuum L. Pakistan Journal of Botany, 42(2), 805-814.
Ozturk, B., Bektas, E., Aglar, E., Karakaya, O., & Gun, S. (2018). Cracking and quality attributes of jujube fruits as affected by covering and pre-harvest Parka and GA3 treatments. Scientia Horticulturae, 240, 65-71. https://doi.org/10.1016/j.scienta.2018.06.004
Paroussi, G., Voyiatzis, D., Paroussis, E., & Drogoudi, P. (2002). Growth, flowering and yield responses to GA3 of strawberry grown under different environmental conditions. Scientia Horticulturae, 96(1-4), 103-113. https://doi.org/10.1016/S0304-4238(02)00058-4
Ranganna, S. (1977). Manual of analysis of fruit and vegetable products. New Delhi, Tata McGraw-Hill.
Rasheed, M. K. (2009). Role of boron in plant growth: a review. Journal of Agricultural Research, 47(3), 329-338.
Safdary, A. J. (2015).Effects of gibberellic acid, 2, 4-diclorophenoxyacetic acid and potassium sulfate on fruit growth, shot berry and fruit quality of grapevine cv. Siah-e-Samarghandi. Master’s thesis. Shiraz, Iran, Shiraz University.
Sardoei, A. S., Shahadadi, F., Vakili, M. A., & Gholamshahi, S. (2014). Effects of gibberellic acid (GA3) on phenolic compounds and antiradical activity of marigold (Calendula officinalis). International Journal of Biosciences, 4(3), 80-84.
Sarkhosh, A., Zamani, Z., Fatahi, R., & Ebadi, A. (2006). RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Scientia Horticulturae, 111(1), 24-29. https://doi.org/10.1016/j.scienta.2006.07.033
Selah Varzi, Z,. Talai, A,. & Fatahi Moqadam, M. R. (2003). Investigation the effect of gibberllic acid on physicochemical properties and storage of pomegranate fruit cv. Shahnaz under low irrigation. Horticulture Science, 30,- 2008-4730.
Shukla, H. S., Kumar, V., & Tripathi, V. K. (2011). Effect of gibberellic acid and boron on development and quality of aonla fruits' Banarasi'. Acta Horticulturae, 890, 375-380.
Stone, H., & Sidel, J. L. (1985). Sensory evaluation practices. Academic Press. San Diego.
Tehranifar, A., & Mahmoudi Tabar, S. (2009). Foliar application of potassium and bron during pomegranate (Punica granatum) fruit development can improve fruit quality. Horticulture, Environment, and Biotechnology, 50(3), 191-196.
Thurzo, S., Szabo, Z., Nyeki, J., Nagy, P. T., Silva, A. P., & Goncalves, B. (2008). Effect of boron and calcium sprays on photosynthetic pigments, total phenols and flavonoid content of sweet cherry (Prunus avium L.). In VI International Symposium on Mineral Nutrition of Fruit Crops, 868, 457-462.
Tuan, N. M., & Chung–Ruey, Y. (2013). Effect of gibberellic acid and 2, 4-dichlorophenoxyacetic acid on fruit development and fruit quality of wax apple. International Journal of Biology Food, Veterinary and Agriculture Engineering, 7(1), 28-36.
Turkmen, N., Sari, F., & Velioglu, Y. S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry, 93(4), 713-718. https://doi.org/10.1016/j.foodchem.2004.12.038
Umar, S., Bansal, S., Imas, P., & Magen, H. (1999). Effect of foliar fertilization of potassium on yield, quality, and nutrient uptake of groundnut. Journal of Plant Nutrition, 22(11), 1785-1795. https://doi.org/10.1080/01904169909365754
Voss, D. H. (1992). Relating colorimeter measurement of plant color to the Royal Horticultural Society Colour Chart. Horticulture Science, 27(12), 1256-1260.
Wagner, G. J. (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology, 64(1), 88-93.
Wang, Y., Lau, M., Tang, J. & Mao, R. (2004). Kinetics of chemical marker M-1 formation in whey protein gels for developing sterilization processes based on dielectric heating. Journal of Food Engineering. 64(1), 111-118.
Weksler, A., Dagar, A., Friedman, H., & Lurie, S. (2009). The effect of gibberellin on firmness and storage potential of peaches and nectarines. In VII International Peach Symposium, 962 (pp. 591-595).        
Wojcik, P., Wojcik, M., & Klamkowski, K. (2008). Response of apple trees to boron fertilization under conditions of low soil boron availability. Scientia Horticulturae, 116(1), 58-64.
Xuan, H., Streif, J., & Bangerth, F. (2000). Effect of boron applications on physiological disorders in ‘Conference’pears during CA-storage. In IV International Conference on Postharvest Science. 553 (pp. 249-254).
Yadav, A., Kumari, R., Yadav, A., Mishra, J. P., Srivatva, S., & Prabha, S. (2016). Antioxidants and its functions in human body-A Review. Research in Environment and Life Science, 9(11), 1328-1331.
Yadav, S., Shukla, H. S., & Ram, R. A. (2010). Studies on foliar application of NAA, GA3, Boric acid, and Ca (NO3) 2 on fruit retention, growth, yield and quality of Aonla (Emblica officinalis Gaertn.) cv. Banarasi. Society for the Advancment of Horticulture, 23(2), 64-67.
Zohary, D., & Spiegel-Roy, P. (1975). Beginnings of fruit growing in the Old World. Science, 187(4174), 319-327. https://doi.org/10.1126/science.187.4174.319