Document Type : Original Article

Authors

Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

Abstract

Purpose: Soil salinity is a prevalent abiotic stress that adversely affects crop productivity worldwide. Salinity is an environmental stress that limits growth and development in fruits and vegetables due to increasing osmotic pressure, imbalancing of nutrients and toxicity of some special ions. Tomato (Lycopersicon esculentum) is one of the most important fruits and vegetables. Research method: In this study, effect of salt stress (NaCl) on eight cultivars of  tomato (king  stone, Caligen, Super Strain B, Primo Early, Early Urbana VF, Early Urbana 111, Cal-j-N3 and Peto Early CH) were investigated. Plants were cultured in hydroponic conditions with five treatments of NaCl including 0, 30, 60, 90 and 120 mM. Each test has repeated three times. The growth indices, Ions, chlorophyll, soluble sugar and proline contents, were measured in roots, stems, petioles and leaves. Main findings: The results showed that, sodium and chloride contents increased in all parts of the cultivars with increasing NaCl concentration. However nitrate and potassium contents decreased. Proline and soluble sugars contents increased in leaves under salt stress too. In Primo- Early, Early Urbana 111, Cal-j-N3 and Petoearly CH, soluble sugar content increased with increasing NaCl concentration up to 90 mM. In all varieties, high salinity reduced chlorophyll a and b, total chlorophyll, carotenoids contents and growth indices. As far as the measured factors are concerned, it seems that Early Urbana VF and Super Strain B showed a high capacity to tolerate salinity stress. Limitations: No limitations were founded. Originality/Value: This is an opportunity to identify salinity-tolerant tomato cultivars that play an important role in the cultivation and production of quality crops.                                                                                   

Keywords

Main Subjects

Abd El-Aziz, N. G., MazherAzz, A. M., & El-Habba, E. (2006). Effect of foliar spraying ascorbic acid on growth and chemical constituents of Khayasene galensis growth under salt condition. American Eurasian Journal of Agricultural and Environmental Sciences, 1(3), 207-214.
Al-Sobhi, O. A., Al-Zahrani, H. S., & Al-Ahmadi, S. B. (2006). Effect of salinity on chlorophyll and carbohydrate contents of Calotropis procera seedlings. Journal of Basic and Applied Sciences, 105-115.
Amira, M. S., & Qados, A. (2011). Effect of salt stress on plant growth and metabolism of bean plant Vicia faba. Journal of the Saudi Society of Agricultural Science, 10, 7-15.
Ashraf, M. Y., & Bhatti, A. S. (2000). Effect of salinity on growth and chlorophyll content in rice. Pakistan Journal of Scientific and Industrial Research, 43, 130-131.
Ashraf, M. (1999). Interactive effect of salt (NaCl) and nitrogen form of growth, water relations and photosynthesis capacity of sunflower (Helianthus annus L.). Annals of Applied Biology, 135, 509-513.
Azarafshan, M., & Abbaspour, N. (2014). Growth and physiological parameters under salinity stress in Lotus corniculatus. Iranian Journal of Plant Physiology, 4(2), 991-997. https://doi.org/10.22034/IJPP.2014.540309
Bartels, D., & Sunkar, R. (2005). Drought and tolerance in plants. Critical Reviews in Plant Science, 24, 23-58.
Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
Blumwald, E., Aharon, G. S., & Apse, M. P. (2000). Sodium transport in plant cells. Biochim BiophysActa, 1465, 140-151.
Bor, M., Ozdemir, F., & Turkan, I. (2003). The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet (Beta vulgaris L.) and wild beet (Beta maritimal L.). Plant Science, 164, 77-84. https://doi.org/10.1016/s0168-9452(02)00338-2
Bray, A. (2003). Molecular responses to water. Plant Physiology, 103, 1035-1043.
Carden, D. E., Walker, D. J., Flowers, T. J., & Miller, A. J. (2003). Single-cell measurements of the contributions of cytosolic Na+ and K+ to salt tolerance. Plant Physiology, 131, 657-661. https://doi.org/10.1104/pp.011445
Cataldo, D. A., Maroon, M., Schrader, L. E., & Youngs, V. L. (1975). Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis, 6(1), 71-80. https://doi.org/10.1080/00103627509366547
Chartzoulakis, K., & Klapaki, G. (2000). Response of two green house pepper hybrids to NaCl salinity during different growth stages. Scientia Horticulturae, 86, 247-260. https://doi.org/10.1016/s0304-4238(00)00151-5
 Chen, Z., Newman, I., Zhou, M., Mendham, N., Zhang, G., & Shabala, S. (2005). Screening plants for salt tolerance by measuring K+ flux: a case study for barley. Plant Cell Environment, 28, 1230-1246. https://doi.org/10.1111/j.1365-3040.2005.01364.x 
Chen, Z., Zhou, M., Newman, I., Mendham, N., Zhang G., & Shabala, S. (2007). Potassium and sodium relations in salinised barley tissues as a basis of differential salt tolerance. Functional Plant Biology, 34, 150-162. https://doi.org/10.1071/fp06237
Cherel, I. (2004). Regulation of K+ channel activities in plants: from physiological to molecular aspects. Journal of Experimental Botany, 55, 337-351. https://doi.org/10.1093/jxb/erh028
Cram, W. J. (1983). Chloride accumulation as a homeostatic system: set points and perturbation. Journal of Experimental Botany, 34, 1484-1502.  https://doi.org/10.1093/jxb/34.11.1484
Cramer, M. D., Schierholt, A., Wang, Y. Z., & Lips, S. H. (1995). The influence of salinity on the utilization of root anaplerotic carbon and nitrogen metabolism in tomato seedlings. Journal of Experimental Botany, 46, 1569-1577. https://doi.org/10.1093/jxb/46.10.1569
Dubios, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substance. Analytical Chemistry, 28(3), 350-356.       https://doi.org/ 10.1021/ac60111a017
Eker, S., Comertpay, G., Konuskan, O., Ulger, A. C., Ozturk, L., & Cakmak, I. (2006). Effect of salinity stress on dry matter production and ion accumulation in hybrid maize varieties. Turkish Journal of Agriculture and Forestry, 30, 1-9.
El-Hendaway, S. E., Hu, Y., Yakout, G. M., Awad, A. M., Hafiz, S. E., & Schmidhalter, U. (2005). Evaluating salt tolerance of wheat genotypes using the multiple parameters. The European Journal of Agronomy, 22, 243-253. https://doi.org/10.1016/j.eja.2004.03.002
Essa, T. A. (2002). Effect of salinity stress on growth and nutrient composition of three soybean (Glycine max L. Merrill) cultivars. Journal of Agronomy and Crop Science, 188, 86-93. https://doi.org/10.1046/j.1439-037x.2002.00537.x   
FAO, (2000). Production year book. Food and Agriculture Organization of the United Nations, Rome.
FAO, (1995). Production year book. Food and Agriculture Organization of the United Nations, Vol. 48.
Ferreira-Silva, S. L., Silveira, J., Voigt, E., Soares, L., & Viegas, R. (2008). Changes in physiological indicators associated with salt tolerance in two contrasting cashew rootstocks.Brazilian Journal of Plant Physiology, 20(1), 51-59.  https://doi.org/10.1590/s1677-04202008000100006
Fisarakis, I., Chartzoulakis, K., & Stavrakas, D. (2001). Response of sultana vines (V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agricultural, Water Management, 51, 13-27.  https://doi.org/10.1016/s0378-3774(01)00115-9 
Fozouni, M., Abbaspour, N., & Doulati Baneh, H. (2012). Leaf water potential, photosynthetic pigments and compatible solutes alterations in four grape cultivars under salinity. Vitis, 51(3), 147-152.
Garg, B. K., & Gupta, I. C. (1997). Saline Wastelands Environment and Plants Growth. Scientific Publisher’s Journal Department, 287 pp.
Hajer, A. S., Malibari, A. A., Al-Zahrani, H. S., & Almaghrabi, O. A. (2006). Responses of three tomato cultivars to sea water salinity I. Effect of salinity on the seedling growth. Africa Journal Biotechnology, 5, 855-861.
Hoekstra, F. A., Golovina, E. A., & Buitink J. (2001). Mechanisms of plant desiccation tolerance. Trends in Plant Science, 6, 431-438.
Jian Kang, Z. (2002). Plant salt tolerance. Trends in Plant Science, 6(2), 66-71.
Kerepesi, I., & Galiba, G. (2000). Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Science, 40, 482-487. https://doi.org/10.2135/cropsci2000.402482x
Khatkar, D., & Kuhad, M. S. (2000). Short-term salinity induced changes in two wheat cultivars at different growth stages. Biologia Plantarum, 43, 629-632.
Khavarinejad, R. A., & Mostofi, Y. (1998). Effects of NaCl on photosynthetic pigments, saccharides, and chloroplast ultra structure in leaves of tomato cultivars. Photosynthetica, 35, 151-154.
Kumar, P. A., & Bandhu, D. A., (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60, 324-349. https://doi.org/10.1016/j.ecoenv.2004.06.010
Kusvuran, S., Yasar, F.,  Ellialtioglu, S., & Abak, K. (2007). Utilizing some of screening methods in order to determine of tolerance of salt stress in the melon (Cucumis melo L.). Research Journal of Agriculture and Biological Sciences, 3(1), 40-45.
Leonova, T. G., Goncharova, E. A., Khodorenko, A. V., & Babakov, A. V. (2005). Characteristics of salt-tolerant and saltsusceptible cultivars of barley. Russian Journal of Plant Physiology, 52, 774-778. https://doi.org/10.1007/s11183-005-0114-6
Lichtenthaler, H. K., & Wellburn, A. R. (1983). Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11(5), 591-592. https://doi.org/10.1042/bst0110591
Maas, E. V. (1986). Salt tolerance of plants. International Journal of Applied Agricultural Research, 1, 12-26.
Massa, D., Mattson, N. S., & Lieth, H. J. (2009). Effects of saline root environment (NaCl) on nitrate and potassium uptake kinetics for rose plants: a Michaelis–Menten modelling approach. Plant Soil, 318, 101-115. https://doi.org/10.1007/s11104-008-9821-z
Meloni, D. A., Oliva, M. A., Ruiz, H. A., & Martinez, C. A. (2001). Contribution of proline and inorganic solutes to osmotic adjustment in cotton under salt stress. Journal of Plant Nutrition, 24, 599-612. https://doi.org/10.1081/pln-100104983
Mitsuya, S., Kawasaki, M., Taniguchi, M., & Miyake, H. (2003). Relationship between salinity-induced damages and aging in rice leaf tissues. Plant Product Science, 6, 213-218.  https://doi.org/10.1626/pps.6.213
Munns, R., James, R. A., & Lauchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. The Journal of Experimental Botany, 57, 1025-1043. https://doi.org/10.1093/jxb/erj100
Nedjimi, B. (2011). Is salinity tolerance related to osmolytes accumulation in Lygeum spartum L. seedlings? Journal of the Saudi of Agricultural Science, 10, 81-87. https://doi.org/10.1016/j.jssas.2011.03.002
Parida, A. K., & Das, A. B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology & Environmental Safety, 60, 324-349.
Parida, A. K., Das, A. B., & Mittra, B. (2004). Effects of salt on growth, ion accumulation, photosyntesis and leaf anatomy of the mangrove Bruguiera parviflora. Trees Structure and Function, 18, 167-174.  https://doi.org/10.1007/s00468-003-0293-8
Prado, F. E., Boero, C., Gallardo, M., & Gonzalez, J. A., (2000). Effect of NaCl on germination, growth, andsoluble sugar content in Chenopodium quinoa Willd. seeds. Botanical Bulletin- Academia Sinica, 41, 27-34.
Salam, A., Hollington, P. A., Gorham, J., Wyn Jones R. G., & Gliddon, C. (1999). Physiological genetics of salt tolerance in wheat (Triticum aestivum L.): performance of wheat varieties, inbred lines and reciprocal F1 hybrids under saline conditions. Journal of Agronomy and Crop Science, 183, 145-152. https://doi.org/10.1046/j.1439-037x.1999.00361.x
Savvas, D., Mantzos, N., Barouchas, P. E., Tsirogiannis, I. L., Olympios, C., & Passam, H. C. (2007). Modelling salt accumulation by a bean crop grown in a closed hydroponic system in relation to water uptake. Scientia Horticulturea, 111, 311–318. https://doi.org/10.1016/j.scienta.2006.10.033
Singh, S. K., Sharma, H. C., Goswami, A. M., Datta, S. P., & Singh, S. P. (2000). In vitro growth and leaf composition of grapevine cultivars as affected by sodium chloride. Biologia Plantarum, 43, 283-286.
Sotiropoulos, T. E., Therios, I. N., Almaliotis, D., Papadakis, I., & Dimassi, K. N. (2006). Response of cherry rootstocks to boron and salinity. Journal of Plant Nutrition, 29, 1691-1698.  https://doi.org/10.1080/01904160600851650
Stepien, P., & Klobus, G. (2006). Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress. Biological Plantarum, 50(40), 610-616. https://doi.org/10.1007/s10535-006-0096-z
Suhayda, C. G., Giannini, J. L., Briskin, D. P., & Shannon, M. C. (1990). Elestrostatic changes in Lycopersicon esculentum root plasma membrane resulting from salt stress. Plant Physiology, 93, 471-478. https://doi.org/10.1104/pp.93.2.471 
Teakel, N. L., Real D., & Colmer T. D. (2006). Growth and ion relations in response to combined salinity and water logging in perennial forage legumes Lotus corniculatus and Lotus tenuis. Plant Soil, 289, 369-383. https://doi.org/10.1007/s11104-006-9146-8 
Teakle, N. L., & Tyerman, S. D. (2010). Mechanisms of Cl- transport contributing to salt tolerance. Plant Cell Environment, 33, 566-589.  https://doi.org/10.1111/j.1365-3040.2009.02060.x
Teymouri, A., Moghadam, M., Heidari Sharifabad, H., Jafari, M., & Azarnivand, H. (2005). Effect of salinity levels on seed germination in three Salsola species. Iranian Journal Natural Research, 58(3), 701-710.
Tester, M., & Davenport, R. (2003). Na+ tolerance and Na+ transport in higher plants. Annals of Botany, 91, 503-527. https://doi.org/10.1093/aob/mcg058
Yancey, P. H., Clark, M. E., Hand, S. C., Bowlus, R. D., & Somero, G. N. (1982). Living with water stress: evolution of osmolyte systems. Science, 217, 1214-1222. https://doi.org/10.1126/science.7112124
Yasar, F., Uzal, O., Tufenkci, S., & Yildiz, K. (2006). Ion accumulation in different organs of green bean genotypes grown under salt stress. Plant Soil and Environment, 52(10), 476-480. https://doi.org/10.17221/3469-pse