Influence of low-temperature stress on the production of zearalenone by three Fusarium species in vitro

Seirafinia, Mohammad; Omidi, Arash; Rasooli, Aria; Mohebbi, Mehdi; Hoseinzadeh, Saeed

doi:10.22077/jhpr.2020.3309.1137

Document Type : Short Communication Article

Authors

¹ Department of Animal Health Management, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

² Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

https://doi.org/10.22077/jhpr.2020.3309.1137

Abstract

Purpose: Comparison and determination of ZEN production capacity of F. oxysporum, F. graminearum, and F. solani. The influence of low temperature stress and incubation time on the production of ZEN was also investigated. Research Method: Production of ZEN was evaluated by incubating media cultures at a constant temperature of 25°C for a period of 14 days before being incubated at 8°C for 14 days (Low-temperature stress/ LTS). The second set of the samples was incubated at constant 25°C for 4 weeks’ time (constant- temperature/ CT). HPLC and a fluorescence detector were employed to measure the concentration of ZEN. The species-specific pair of primers was used to perform a PCR assay on the fungal DNA. Findings: The higher levels of ZEN were seen at LTS compared with CT in three Fusarium species. The highest ZEN was produced in F. oxysporum media cultures. The significant production of ZEN was seen when F. oxysporum incubated at LTS compared with CT (P≤0.05). The highest and the lowest levels of ZEN were seen in F. oxysporum and F. solani and the difference of ZEN production by them were significant in CT and LTS, respectively (P≤0.05). ZEN production for the F. graminearum was in the intermediate level. Presence of PKS4 gene was confirmed by PCR in these Fusarium species. Limitations: Higher cost of ZEN detection by HPLC was a limitation. Originality/Value: Production of ZEN in LTS and CT by the Fusarium evaluated species was confirmed. However, LTS stimulate ZEN production in Fusarium media cultures, especially for F. oxysporum.

Keywords

Main Subjects

Plant Stress

References

Antonissen, G., Van Immerseel, F., Pasmans, F., Ducatelle, R., Janssens, G. P., & De Baere, S. (2015). Mycotoxins deoxynivalenol and fumonisins alter the extrinsic component of intestinal barrier in broiler chickens. Journal of Agricultural and Food Chemistry, 63(50), 10846-10855. https://doi.org/10.1021/acs.jafc.5b04119

Beev, G., Denev, S., & Bakalova, D. (2013). Zearalenone-producing activity of Fusarium graminearum and Fusarium oxysporum isolated from Bulgarian wheat. Bulgarian Journal of Agricultural Science, 19(2), 255-259.

Caldwell, R. W., Tuite, J., Stob, M., & Baldwin, R. (1970). Zearalenone production by Fusarium species. Applied and Environmental Microbiology, 20(1), 31-34. https://doi.org/10.1128/aem.20.1.31-34.1970

Döll, S., & Dänicke, S. (2011). The Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) in animal feeding. Preventive Veterinary Medicine, 102(2), 132-145. https://doi.org/10.1016/j.prevetmed.2011.04.008

Duverger, F., Bailly, S., Querin, A., Pinson-Gadais, L., Guerre, P., & Bailly, J. D. (2011). Influence of culture medium and incubation time on the simultaneous synthesis of Deoxynivalenol and zearalenone by Fusarium graminearum. Revenue Medical and Veterinary, 162, 93-97.

Greenhalgh, R., Neish, G. A., & Miller, J. D. (1983). Deoxynivalenol, acetyl deoxynivalenol, and zearalenone formation by Canadian isolates of Fusarium graminearum on solid substrates. Applied and Environmental Microbiology, 46(3), 625-629. https://doi.org/10.1128/aem.46.3.625-629.1983

Häggblom, P., & Nordkvist, E. (2015). Deoxynivalenol, zearalenone, and Fusarium graminearum contamination of cereal straw; field distribution; and sampling of big bales. Mycotoxin Research, 31(2), 101-107. https://doi.org/10.1007/s12550-015-0220-z

Jimenez, M., Manez, M., & Hernandez, E. (1996). Influence of water activity and temperature on the production of zearalenone in corn by three Fusarium species. International Journal of Food Microbiology, 29(2-3), 417-421. https://doi.org/10.1016/0168-1605(95)00073-9

Kokkonen, M., Ojala, L., Parikka, P., & Jestoi, M. (2010). Mycotoxin production of selected Fusarium species at different culture conditions. International Journal of Food Microbiology, 143(1-2), 17-25. https://doi.org/10.1016/j.ijfoodmicro.2010.07.015

Lahouar, A., Marin, S., Crespo-Sempere, A., Saïd, S., & Sanchis, V. (2017). Influence of temperature, water activity and incubation time on fungal growth and production of ochratoxin A and zearalenone by toxigenic Aspergillus tubingensis and Fusarium incarnatum isolates in sorghum seeds. International Journal of Food Microbiology, 242, 53-60. https://doi.org/10.1016/j.ijfoodmicro.2016.11.015

Lysøe, E., Klemsdal, S. S., Bone, K. R., Frandsen, R. J., Johansen, T., Thrane, U., & Giese, H. (2006). The PKS4 gene of Fusarium graminearum is essential for zearalenone production. Applied and Environmental Microbiology, 72(6), 3924-3932. https://doi.org/10.1128/aem.00963-05

Manka, M., Visconti, A., Chełkowski, J., & Baottalico, A. (1985). Pathogenicity of Fusarium isolates from wheat, rye and triticale towards seedlings and their ability to produce trichothecenes and zearalenone. Journal of Phytopathology, 113(1), 24-29. https://doi.org/10.1111/j.1439-0434.1985.tb00820.x

Martins, M. L., & Martins, H. M. (2002). Influence of water activity, temperature and incubation time on the simultaneous production of deoxynivalenol and zearalenone in corn (Zea mays) by Fusarium graminearum. Food Chemistry, 79(3), 315-318. https://doi.org/10.1016/s0308-8146(02)00147-4

Milano, G. D., & López, T. A. (1991). Influence of temperature on zearalenone production by regional strains of Fusarium graminearum and Fusarium oxysporum in culture. International Journal of Food Microbiology, 13(4), 329-333. https://doi.org/10.1016/0168-1605(91)90092-4

Molto, G. A., Gonzalez, H. H. L., Resnik, S. L., & Gonzalez, A. P. (1997). Production of trichothecenes and zearalenone by isolates of Fusarium spp. from Argentinian maize. Food Additives and Contaminants, 14(3), 263-268. https://doi.org/10.1080/02652039709374523

Muthomi, J. W., Ndung’u, J. K., Gathumbi, J. K., Mutitu, E. W., & Wagacha, J. M. (2008). The occurrence of Fusarium species and mycotoxins in Kenyan wheat. Crop Protection, 27(8), 1215-1219. https://doi.org/10.1016/j.cropro.2008.03.001

Richardson, K. E., Hagler, W. M., Campbell, C. L., & Hamilton, P. B. (1985). Production of zearalenone, T-2 toxin, and deoxynivalenol by Fusarium spp. isolated from plant materials grown in North Carolina. Mycopathologia, 90(3), 155-160. https://doi.org/10.1007/bf00436731

Ryu, D., & Bullerman, L. B. (1999). Effect of cycling temperatures on the production of deoxynivalenol and zearalenone by Fusarium graminearum NRRL 5883. Journal of Food Protection, 62(12), 1451-1455. https://doi.org/10.4315/0362-028x-62.12.1451

Sim, J. H., Tian, F., Jung, S. Y., Auh, J. H., & Chun, H. S. (2018). Multiplex polymerase chain reaction assays for the detection of the zearalenone chemotype of Fusarium species in white and brown rice. International Journal of Food Microbiology, 269, 120-127. https://doi.org/10.1016/j.ijfoodmicro.2018.02.003

Skrinjar, M., Stubblefield, R. D., Stojanović, E., & Dimić, G. (1995). Occurrence of Fusarium species and zearalenone in dairy cattle feeds in Vojvodina. Acta Veterinaria Hungarica, 43(2-3), 259-267.

Streit, E., Naehrer, K., Rodrigues, I., & Schatzmayr, G. (2013). Mycotoxin occurrence in feed and feed raw materials worldwide: long‐term analysis with special focus on Europe and Asia. Journal of the Science of Food and Agriculture, 93(12), 2892-2899. https://doi.org/10.1002/jsfa.6225

Szabó, B., Varga, M., György, A., Mesterházy, Á., & Tóth, B. (2016). Role of Fusarium species in mycotoxin contamination of maize. Review on Agriculture and Rural Development, 5(1-2), 104-108.

Wilson, D. (2010). Clinical veterinary advisor-e-book: the horse. Elsevier Health Sciences.

Wu, L., Qiu, L., Zhang, H., Sun, J., Hu, X., & Wang, B. (2017). Optimization for the production of deoxynivalenoland zearalenone by Fusarium graminearum using response surface Methodology. Toxins, 9(2), 57. https://doi.org/10.3390/toxins9020057

Journal of Horticulture and Postharvest Research

Influence of low-temperature stress on the production of zearalenone by three Fusarium species in vitro

References

References

Volume 4, Issue 1 - Serial Number 9
March 2021
March 2021
Pages 63-70

Influence of low-temperature stress on the production of zearalenone by three Fusarium species in vitro

References

References

Volume 4, Issue 1 - Serial Number 9March 2021March 2021Pages 63-70

Volume 4, Issue 1 - Serial Number 9
March 2021
March 2021
Pages 63-70