Impact of exogenous arginine, cysteine and methionine on the postharvest senescence of six green leafy vegetables

Sohail, Muhammad; Wills, Ron B. H.; Bowyer, Michael C.; Pristijono, Penta

doi:10.22077/jhpr.2020.3844.1180

Document Type : Original Article

Authors

School of Environmental and Life Sciences, University of Newcastle, Ourimbah, Australia

https://doi.org/10.22077/jhpr.2020.3844.1180

Abstract

Purpose: This study examined the efficacy of aqueous dips containing the amino acids, L-arginine, L-cysteine and L-methionine, to inhibit the senescence of six leafy green vegetables pak choy, coriander, choy sum, spinach, parsley and rocket. Research method: Pak choy was dipped in amino acid solutions from 2-100 mM to determine the optimum concentration that inhibited senescence. The other vegetables were dipped in solutions with the optimal concentration. Senescence of the vegetables was assessed during storage at 10 °C in air containing 0.1 µL L¹ ethylene by determining loss of green colour (designated as green life), ethylene production and respiration rates. Findings: For each amino acid, a dipping concentration of 5 mM was found optimal to inhibit senescence as shown by an extended green life and reduced ethylene production and respiration rates of all vegetables to a similar extent, except methionine which did not show a significant effect with rocket, and for spinach only reduced ethylene production. Limitations: No limitations were encountered. Originality/Value: Arginine and cysteine showed considerable potential for commercial use to extend the market life of many green vegetables and with their Generally Recognized As Safe (GRAS) status the amino acids should be a safe, consumer-acceptable treatment.

Graphical Abstract

Keywords

Main Subjects

Postharvest Biology and Technology

References

Al Ubeed, H.M.S., Wills, R.B.H., Bowyer, M.C., & Golding, J.B. (2019a). Inhibition of postharvest senescence of green leafy vegetables by exogenous D-cysteine and L-cysteine as precursors of hydrogen sulphide. The Journal of Horticultural Science and Biotechnology, 94(5), 620-626. https://doi.org/10.1080/14620316.2019.1591171

Al Ubeed, H.M.S., Wills, R.B.H., Bowyer, M.C., Vuong, Q.V., & Golding, J.B. (2019b). Effects of hydrogen sulphide, nitric oxide and ethylene on postharvest deterioration of pak choy. Acta Horticulturae, 1256, 115-120. https://doi.org/10.17660/ActaHortic.2019.1256.16

Al Ubeed, H.M.S., Wills, R.B.H., Bowyer, M.C., Vuong, Q.V., & Golding, J.B. (2017). Interaction of exogenous hydrogen sulphide and ethylene on senescence of green leafy vegetables. Postharvest Biology and Technology, 133, 81-87. https://doi.org/10.1016/j.postharvbio.2017.07.010

Ali, H.M., El-Gizawy, A.M., El-Bassiouny, R.E.I., & Saleh, M.A. (2016). The role of various amino acids in enzymatic browning process in potato tubers, and identifying the browning products. Food Chemistry, 192, 879-885. https://doi.org/10.1016/j.foodchem.2015.07.100

Aroca, A., Gotor, C., & Romero, L.C. (2018). Hydrogen Sulfide Signaling in Plants: Emerging Roles of Protein Persulfidation. Frontiers in Plant Science, 9 (1369). https://doi.org/10.3389/fpls.2018.01369

Babalar, M.,Pirzad, F., Sarcheshmeh, M.A.A., Talaei, A., & Lessani, H. (2018). Arginine treatment attenuates chilling injury of pomegranate fruit during cold storage by enhancing antioxidant system activity. Postharvest Biology and Technology, 137, 31-37. https://doi.org/10.1016/j.postharvbio.2017.11.012

Corpas, F.J., González-Gordo, S., Cañas, A., & Palma, J.M. (2019). Nitric oxide and hydrogen sulfide in plants: which comes first? Journal of Experimental Botany, 70(17), 4391-4404. https://doi.org/10.1093/jxb/erz031

Dussán-Sarria, S., Gaona-Acevedo, A.F., & Hleap-Zapata, J.I. (2017). Effect of use of antioxidants on green plantain slices dominico-harton (Musa AAB Simmonds). Información Tecnológica, 28(4), 3-10. https://dx.doi.org/10.4067/S0718-07642017000400002

Jones, K. (2014). Case studies of hydrogen sulphide occupational exposure incidents in the UK. Toxicology Letters, 231(3), 374-377. https://doi.org/10.1016/j.toxlet.2014.08.005

Li, S.P., Hu, K.D., Hu, L.Y., Li, Y.H., Jiang, A.M., Xiao, F., Han, Y., Liu, Y.S., &. Zhang, H. (2014). Hydrogen sulfide alleviates postharvest senescence of broccoli by modulating antioxidant defense and senescence-related gene expression. Journal of Agricultural and Food Chemistry, 62(5), 1119-1129. https://doi.org/10.1021/jf4047122

Liu, Y.H., Lu, M., Hu, L.F., Wong, P. T.H., Webb, G.D., & Bian, J.S. (2012). Hydrogen sulfide in the mammalian cardiovascular system. Antioxidants and Redox Signaling, 17(1), 141-185. https://doi.org/10.1089/ars.2011.4005

Morris, S.M. (2007). Arginine metabolism: boundaries of our knowledge. The Journal of Nutrition, 137(6), 1602S-1609S. https://doi.org/10.1093/jn/137.6.1602S

Paquay, J.B., Haenen, G.R., Stender, G., Wiseman, S.A., Tijburg, L.B., & Bast, A. (2000). Protection against nitric oxide toxicity by tea. Journal of Agricultural and Food Chemistry, 48(11), 5768-5772. https://doi.org/10.1021/jf981316h

Serrano, M., Zapata, P.J., Martínez-Romero, D., Díaz-Mula, H.M., & Valero, D. (2016). Polyamines as an ecofriendly postharvest tool to maintain fruit quality. In M.W. Siddiqui (Ed.), Eco-Friendly Technology for Postharvest Produce Quality: London, Academic Press. pp. 219-242.

Sharma, S., Pareek, S., Sagar, N.A., Valero, D., & Serrano, M. (2017). Modulatory effects of exogenously applied polyamines on postharvest physiology, antioxidant system and shelf life of fruits: A Review. International Journal of Molecular Sciences, 18(8), 1789. https://doi.org/10.3390/ijms18081789

Sohail, M., Wills, R.B.H., Bowyer, M.C., & Pristijono, P. (2021). Beneficial impact of exogenous arginine, cysteine and methionine on postharvest senescence of broccoli. Food Chemistry, 338, 128055. https://doi.org/10.1016/j.foodchem.2020.128055

Wang, X., Gu, S., Chen, B., Huang, J., & Xing, J. (2017). Effect of postharvest L-arginine or cholesterol treatment on the quality of green asparagus (Asparagus officinalis L.) spears during low temperature storage. Scientia Horticulturae, 225, 788-794. https://doi.org/10.1016/j.scienta.2017.07.058

Wills, R.B.H. (2015). Low ethylene technology in non-optimal storage temperatures. In: Advances in Postharvest Fruit and Vegetable Technology (eds R.B.H. Wills & J.B. Golding) Boca Raton FL CRC Press. Chapter 8, pp167-190. https://doi.org/10.1201/b18489

Wills, R.B.H., Soegiarto, L., & Bowyer, M.C. (2007). Use of a solid mixture containing diethylenetriamine/nitric oxide (DETANO) to liberate nitric oxide gas in the presence of horticultural produce to extend postharvest life. Nitric Oxide, 17(1), 44-49. https://doi.org/10.1016/j.niox.2007.05.003

Wills, R.B.H., Warton, M.A., & Ku, V.V.V. (2000). Ethylene levels associated with fruit and vegetables during marketing. Australian Journal of Experimental Agriculture, 40(3), 465-470. https://doi.org/10.1071/EA99125

Zhang, W., & Jiang, W. (2019). UV treatment improved the quality of postharvest fruits and vegetables by inducing resistance. Trends in Food Science and Technology, 92, 71-80. https://doi.org/10.1016/j.tifs.2019.08.012

Zhang, X., Min, D., Li, F., Ji, N., Meng, D., & Li, L. (2017). Synergistic effects of L-arginine and methyl salicylate on alleviating postharvest disease caused by Botrysis cinerea in tomato fruit. Journal of Agricultural and Food Chemistry, 65(24), 4890-4896. https://doi.org/10.1021/acs.jafc.7b00395

Journal of Horticulture and Postharvest Research

Impact of exogenous arginine, cysteine and methionine on the postharvest senescence of six green leafy vegetables

References

References

Volume 4, Issue 3 - Serial Number 14
September 2021
September 2021
Pages 253-262

Impact of exogenous arginine, cysteine and methionine on the postharvest senescence of six green leafy vegetables

References

References

Volume 4, Issue 3 - Serial Number 14September 2021September 2021Pages 253-262

Volume 4, Issue 3 - Serial Number 14
September 2021
September 2021
Pages 253-262