Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

Dehghani, Rahele; Abbasi, Zahra; Talebi, Majid; Shahmansouri, Emad; Sayed Tabatabaei, Badrodin Ebrahim

doi:10.22077/jhpr.2020.3665.1163

Document Type : Original Article

Authors

¹ Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran, 8415683111

² Horticulture Crops Research Department, Isfahan Agricultural and Natural Resources Research and Education Center, (AREEO), Isfahan, Iran

https://doi.org/10.22077/jhpr.2020.3665.1163

Abstract

Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in 123 onion accessions. Research method: Three cytoplasmic markers cob, accD and MK were used to identify the sterility (S) from the fertility (N) cytoplasm and four nuclear molecular markers (OPT, PsaO, Jnurf-13 and AcSKP1) were used for genotyping of Ms alleles. Findings: The results showed that the two accD and cob markers were quite similar in the detection of the type of cytoplasm with 100% male sterility for male sterile lines and 100% fertility for maintainer lines. The MK marker was able to distinguish T-type cytoplasm as well. Based on the results, the frequency of fertility (N) was much more than the frequency of sterility (S and T) cytoplasm found to be 90% in Dorche (pop.1), 100% in Dorche (pop.2) and Kashan based on marker cob and accD and with MK marker, was found to be 80%, 90% and 82% in Dorche (pop.1), Dorche (pop.2) and Kashan, respectively. Limitations: In this study, nuclear markers were not successful due to not finding linkage disequilibrium with the Ms locus, suggested more markers to be evaluated. Originality/Value: Molecuar markers were very suitable for the indentification of S or N lines. Cytotype (N/S) determination of plants by usingmolecular markers (cob, accD and MK), could easily reduce the population size required for the production of onion hybrid seeds.

Keywords

Main Subjects

Plant Breeding and Biotechnology

References

Bang, H., Cho, D. Y., Yoo, K., Yoon, M., Patil, B. S. & Kim, S. (2011). Development of simple PCR-based markers linked to the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.). Euphytica, 179, 439-449. https://doi.org/10.1007/s10681-010-0342-5.

Cho, K. S., Yang, T., Hong, S., Kwon, Y., Woo, J. & Park, H. (2006). Determination of cytoplasmic male sterile factors in onion plants (Allium cepa L.) using PCR-RFLP and SNP markers. Molecular Cells, 21, 411-417.

Engelke, T., Terefe, D. & Tatlioglu, T. (2003). A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theoretical and Applied Genetics, 107, 162-167. https://doi.org/10.1007/s00122-003-1230-3.

Ferreira, R. R. & Santos, C. A. F. (2018). Partial success of marker-assisted selection of ‘A’and ‘B’onion lines in Brazilian germplasm. Science Horticulture, 242, 110-115. https://doi.org/10.1016/j.scienta.2018.08.002.

Ferreira, R. R., Santos, C. A. F. & Oliveira, V. R. (2017). Fertility restoration locus and cytoplasm types in onion. Genetics and Molecular Research, 16(3),1-12.

Gökçe, A. F. & Havey, M. J. (2002) Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations. Journal of the American Society for Horticultural Science, 127, 944-946. https://doi.org/10.21273/jashs.127.6.944.

Havey, M. J. (1993). A putative donor of S-cytoplasm and its distribution among open-pollinated populations of onion. Theoretical and Applied Genetics, 86, 128-134. https://doi.org/10.1007/bf00223817.

Havey, M. J. & Bark O. H. (1994). Molecular confirmation that sterile cytoplasm has been introduced into open-pollinated Grano onion cultivars. Journal of the American Society for Horticultural Science. 119, 90-93. https://doi.org/10.21273/jashs.119.1.90.

Havey, M. J. (2000). Diversity among male-sterility-inducing and male-fertile cytoplasms of onion. Theoretical and Applied Genetics, 101,778-782. https://doi.org/10.1007/s001220051543.

Havey, M. J. (1995). Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open- pollinated populations of onion. Theoretical and Applied Genetics, 90, 263-268. https://doi.org/10.1007/bf00222212.

Havey, M. J. (2013). Single nucleotide polymorphisms in linkage disequilibrium with the male-fertility restoration (ms) locus in open-pollinated and inbred populations of onion. Journal of the American Society for Horticultural Science, 138, 306-309. https://doi.org/10.21273/jashs.138.4.306.

Huo, Y. M., Liu, B. J., Yang, Y., Miao, J., Gao, L. M., Kong, S. P., Wang, Z. B., Kitano, H. & Wu, X. (2015). AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion. Eurhytica, 204, 711-722. https://doi.org/10.1007/s10681-015-1374-7.

Jones, H. A. & Clarke, A. E. (1943). Inheritance of male sterility in the onion and the production of hybrid seed. Proc. Journal of the American Society for Horticultural Science 43, 189–194.

Jones, H. A., & Davis, G. (1944). Inbreeding and heterosis and their relation to the development of new varieties of onions. USDA Tech Bull 874, 1-28.

Jones, H. A., & Emsweller, S. L. (1936). A male-sterile onion. Proceedings of the American Society for Horticultural Science, 34, 582–585.

Khar, A. & Saini, N. (2016). Limitations of PCR-based molecular markers to identify male-sterile and maintainer plants from Indian onion (Allium cepa L.) populations. Plant Breeding, 135, 519-524. https://doi.org/10.1111/pbr.12373.

Khosa, J. S., McCallum, J., Dhatt, A. S., & Macknight, R. C. (2016). Enhancing onion breeding using molecular tools. Plant Breeding, 135, 9-20. https://doi.org/10.1111/pbr.12330

Kim, S. (2014) A codominant molecular marker in linkage disequilibrium with a restorer-of fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Molecular Breeding, 34, 769-778. https://doi.org/10.1007/s11032-014-0073-8.

Kim, S., Kim, C. W., Park, M. & Choi, D. (2015a). Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.) using a combination of bulked segregant analysis and RNA-seq. Theoretical and Applied Genetics, 128, 2289-2299. https://doi.org/10.1007/s00122-015-2584-z.

Kim, S., Lee, E. T., Cho, D. Y., Han, T., Bang, H., Patil, B. S., Ahn, Y. K., & Yoon, M. K. (2009). Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.). Theoretical and Applied Genetics, 118, 433–441. https://doi.org/10.1007/s00122-008-0909-x.

Kim, S., Park, J., & Yang, T. (2015b). Comparative analysis of the complete chloroplast genome sequences of a normal male-fertile cytoplasm and two different cytoplasms conferring cytoplasmic male sterility in onion (Allium cepa L.). Journal Horticulture Science and Biotechnology, 90, 459-468. https://doi.org/10.1080/14620316.2015.11513210.

Malik, G., Dhatt, A. S. & Malik, A. A. (2017). Isolation of Male Sterile and Maintainer Lines from North-Indian Onion (Allium cepa L.) Populations with the Aid of PCR-Based Molecular Marker. Vegetos-An International Journal of Plant Research, 30(2), 94-99. https://doi.org/10.5958/2229-4473.2017.00142.2.

Murry, M., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research, 8, 4321-4325.

Patil, Y. A., Dalvi, V. S., Borole, V. K., Krishna, B., Dhake, A. V., Sane, P. V. & Sane, A. P. (2016). Molecular characterization of CMS lines in short-day white onions. Acta Horticulture, 1143, 259-268. https://doi.org/10.17660/actahortic.2016.1143.37.

Pike, L. M. (1986). Onion breeding. In: Basset, M. J. (Ed.), Breeding Vegetable Crops. AVI Publishing Company, Westport pp: 357-394.

Ragassi, C. F., Santos, M. D. M., Fonseca, M. E. N., Oliveira, V. R., Buzar, A. G. R., Costa, C. P. & Boiteux, L. S. (2012). Genotyping of polymorphisms associated with male-sterility systems in onion accessions adapted for cultivation in Brazil. Horticultura Brasileira, 30, 409-414. https://doi.org/10.1590/s0102-05362012000300009.

Santos, C. A. F., Leite, D. L., Oliveira, V. R. & Rodrigues, M. A. (2010). Marker-assisted selection of maintainer lines within an onion tropical population. Scientia Agricola, 67, 223-227. https://doi.org/10.1590/s0103-90162010000200015.

Santos, M. D. M., Buzar, A. G. R., Fonseca, M. E. N., Oliveira, V. R., Torres, A. C. & Boiteux, L. S. (2007). Molecular characterization of cytoplasm type (N, S, and T) in accessions of an onion germplasm collection from Brazil. Horticultura Brasileira, 25(1), 1-4. https://doi.org/10.1590/s0102-05362007000400007

Sato, Y. (1998). PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.). Theoretical and Applied Genetics, 96, 367-370. https://doi.org/10.1007/s001220050750.

Schweisguth, B. (1973). Study of a new type of male sterility in onion (Allium cepa L.). Ann Amelior Plant. 23, 221-253.

Shahmansouri, E. & Aminpur, R. (2004). Investigation on the possibility of recognition and non-sexual reproduction of male sterile genotypes in three landrace populations. Final report. Isfahan Agricultural and Natural Resources Research and Education Center, AREEO 16 page.

Shahmansouri, E. & Otroshi, M. (2011). Micropropagation of onion male sterile genotypes. Final report. Institute of Agricultural Biotechnology 34 page.

Terefe, D. T. E. & Tatlioglu, T. (2003). A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L). Theoretical and Applied Genetics, 107, 162-167. https://doi.org/10.1007/s00122-003-1230-3.

Tsujimura, M., Kaneko, T., Sakamoto, T., Kimura, S., Shigyo, M., Yamagishi, H. & Terachi, T. (2019). Multichromosomal structure of the onion mitochondrial genome and a transcript analysis. Mitochondrion, 46, 179-186. https://doi.org/10.1016/j.mito.2018.05.001.

Von Kohn, C., Kiełkowska, A. & Havey, M. J. (2013). Sequencing and annotation of the chloroplast DNAs and identification of polymorphisms distinguishing normal male-fertile and male-sterile cytoplasms of onion. Genome, 56 (12), 737-742. https://doi.org/10.1139/gen-2013-0182.

Journal of Horticulture and Postharvest Research

Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

References

References

Volume 4, Issue 3 - Serial Number 14
September 2021
September 2021
Pages 277-288

Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

References

References

Volume 4, Issue 3 - Serial Number 14September 2021September 2021Pages 277-288

Volume 4, Issue 3 - Serial Number 14
September 2021
September 2021
Pages 277-288