Evaluation of the green synthesis, characterization and antibacterial activity of silver nanoparticles from corm extract of Crocus sativus var. Haussknechtii

Zivyar, Navid; Bagherzade, Ghodsieh; Moudi, Maryam; Manzari Tavakoli, Maryam

doi:10.22077/jhpr.2021.3812.1177

Document Type : Original Article

Authors

¹ Department of Chemistry, Faculty of Science, University of Birjand, Birjand 97179-414, Iran

² Department of Biology, Faculty of Science, University of Birjand, Birjand 97179-414, Iran

³ Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran

https://doi.org/10.22077/jhpr.2021.3812.1177

Abstract

Purpose: To prevent chemical toxicity, biosynthesis of silver nanoparticles has been proposed as a cost-effective and environmentally friendly reducing agent option by corm extract of Crocus sativus var. Haussknechtii and as well as, evaluate their effects against Staphylococcus aureus and Escherichia coli. Research method: Silver nanoparticles were produced in the presence of secondary metabolites of this plant. The nanoparticles were then identified using the technique ultraviolet-visible spectroscopy (UV–Vis), fourier transform infrared (FTIR), field emission scanning electron microscope (FESEM).The antibacterial properties were used against two microorganisms, S. aureus (Gram-positive) and E. coli (Gram-negative), using the agar well propagation method. Findings: The observation of the peak at 450 nm in the UV-Vis spectra for corm synthesized silver nanoparticles reveals the reduction of silver metal ions into silver nanoparticles. The optimum pH and AgNO3 concentrations were 9 and 4 mM, respectively. FESEM images detected the spherical Ag-NPs shape with diverse sizes ranged within 70.70–192.02 nm. Additionally, based on the antibacterial test that has been done for nanoparticles, the mean diameter of the inhibition zone after exposure to S. aureus and E. coli was 22.67±0.58 and 20.00±00 mm, respectively. Limitations: There was no significant limitation to report. Originality/Value: The corm extract of C. haussknechtii is a promising agent for the biosynthesis of almost spherical silver nanoparticles. The synthesized nanoparticles show good Inhibition activity in different concentrations. The AgNPs synthesized by corm extract in high concentrations are found to be high antibacterial activity against two bacterial organisms. This indicates that the increase in nanoparticle diameter is directly related to antimicrobial properties.

Keywords

Main Subjects

Medicinal Plants

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Journal of Horticulture and Postharvest Research

Evaluation of the green synthesis, characterization and antibacterial activity of silver nanoparticles from corm extract of Crocus sativus var. Haussknechtii

References

References

Volume 4, Special Issue - Recent Advances in Saffron - Serial Number 13
August 2021
August 2021
Pages 19-32

Evaluation of the green synthesis, characterization and antibacterial activity of silver nanoparticles from corm extract of Crocus sativus var. Haussknechtii

References

References

Volume 4, Special Issue - Recent Advances in Saffron - Serial Number 13August 2021August 2021Pages 19-32

Volume 4, Special Issue - Recent Advances in Saffron - Serial Number 13
August 2021
August 2021
Pages 19-32