Document Type : Original Article

Authors

1 Department of Horticulture, Gorgan University of Agricultural Sciences & Natural Resources, Iran

2 Fars Research Center, Istahban Fig Research Station, Iran

Abstract

Purpose: Diverse commercial cultivars as well as wild pomegranate genotypes are widespread throughout Iran. Such diversity considered as backbone of breeding programs. This study was aimed to comparative analysis of fruit traits of eight local pomegranate cultivars and a well-known, commercially adapted, “Wonderful” cultivar. Research method: The fruits were harvested and transferred to the laboratory. The fruit, aril and skin parameters were measured and the data was analyzed as completely randomized design with three replications. Findings: The results clearly showed diverse differences among cultivars. The highest fruit weight, length, width, aril weight, aril diameter, aril fresh/dry weights, skin fresh/dry weights were found in “Gavkoshak”. The highest calyx length and skin thickness were recorded in “Galookandeh”. The “Torsh Oud”, “Faroogh”, “Galookandeh” and “Rubab” were detected to have hard seeds. The highest TSS, skin / aril anthocyanin and sucrose content were found in “Wonderful”. The maximum amount of glucose and fructose were observed in the “Rubab”. The results finally showed that “Gavkoshak” and “Rubab” cultivars had the greater ranks in terms of their physical fruit parameters. In terms of chemical properties, the best cultivars were “Wonderful” and “Rubab”. The “Rubab”, “Gavkoshak” and “Wonderful” are recommended as superior cultivars for either pomegranate production or future breeding programs. Limitations: There was no limitation. Originality/Value: The “Wonderful” is an introduced one and the comparative analysis of pomegranates of Fars origin concurrently with this new plant material would be valuable. Furthermore, the pomological traits of these local cultivars were not also studied earlier.

Keywords

Main Subjects

Al-Maiman, S.A. & Ahmad, D. (2002). Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chemistry, 76(4), 437–441. https://doi.org/10.1016/S0308-8146(01)00301-6.
Amir, R., Borochov-Neori, H., Tian, L., & Holland, D. (2019). The biodiversity of different traits of pomegranate fruit peels from a broad collection of diverse cultivars. Scientia Horticulturae, 246, 842-848. https://doi.org/10.1016/j.scienta.2018.10.048.
Ashwell, G. (1957). Colorimetric analysis of sugars. In Methods in enzynzology, (3), pp: 73-105.
Ashrafi, S. H., Bodaghi, H. & Rezae, M. (2023). Morphological diversity of indigenous wild pomegranate (Punica granatum L. var. spinosa) accessions from northeast of Iran. Food Science & Nutrition, (11)2, 1001–1012. https://doi.org/10.1002/fsn3.3135.
Barzegar, M., Fadavi, A. & Azizi, M.H. (2004). An investigation on the physico-chemical composition of various pomegranates (Punica granatum L.) grown in Yazd. Iranian Journal of Food Science and Technology, 1(2), 9–14.
Beigi, F., Abdusi, O., Ghasemi, A. & A. Autumn (2012). Evaluation of some local cultivars of Iranian pomegranate for processing and conversion industries. Journal of Food Science and Nutrition. 9 (4), 95-85.
Borochov-Neori, H., Judeinstein, S., Tripler, E., Harari, M., Greenberg, A., Shomer, I. & Holland, D. (2009). Seasonal and cultivar variations in antioxidant and sensory quality of pomegranate (Punica granatum L.) fruit. Journal of Food Composition and Analysis, 22(3), 189–195. https://doi.org/10.1016/j.jfca.2008.10.011.
Cam, M., Hisil, Y. & Durmaz, G. (2009). Characterisation of pomegranate juices from ten cultivars grown in Turkey’, International Journal of Food Properties, 12(2), 388–395. https://doi.org/10.1080/10942910701813917.
 Cea, I. (2011). Caracterización física, química y sensorial de frutos de granado cv. Wonderful provenientes de tres regiones de Chile’, p: 48. https://doi.org/repositorio.uchile.cl/handle/2250/112287.
Chang, C.C., Yang, M.H., Wen, H.M. & Chern, J.C. (2002). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3), 178–182.
Davise, F. & Albrigo, L. (1994). Citrus, CAB. International, USA.
Fawole, O.A. & Opara, U.L. (2013). Effects of maturity status on biochemical content, polyphenol composition and antioxidant capacity of pomegranate fruit arils (cv. ’Bhagwa’). South African Journal of Botany, 85, 23–31. https://doi.org/10.1016/j.sajb.2012.11.010.
Fernandes, L., Pereira, J.A., Lopéz-Cortés, I., Salazar, D.M., González-Álvarez, J. & Ramalhosa, E. (2017). Physicochemical composition and antioxidant activity of several pomegranate (Punica granatum L.) cultivars grown in Spain. European Food Research and Technology, 243(10), 1799–1814. https://doi.org/10.1007/s00217-017-2884-4.
Ferrara, G., Giancaspro, A., Mazzeo, A., Giove, S.L., Matarrese, A.M.S., Pacucci, C., Punzi, R., Trani, A., Gambacorta, G., Blanco, A. & Gadaleta, A. (2014). Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy.Scientia Horticulturae, 178, 70–78. https://doi.org/10.1016/j.scienta.2014.08.007.
Fuleki, T. & Francis, F.J. (1968). Quantitative methods for anthocyanins. 1. Extraction and determination of total anthocyanin in cranberries. Journal of Food Science, 33(1), 72–77. https://doi.org/10.1111/j.1365-2621.1968.tb00888.x.
Gadže, J., Voća, S., Čmelik, Z., Mustać, I., Ercisli, S. & Radunić, M. (2012). Physico-chemical characteristics of main pomegranate (Punica granatum L.) cultivars grown in Dalmatia region of Croatia. Journal of Applied Botany and Food Quality, 85(2), 202–206.
Gozlekci, S. & Kaynak, L. (2000). Physical and chemical changes during fruit development and flowering in pomegranate (Punica granatum L.) cultivar Hicaznar grown in Antalya region. Options Méditerranéennes. Série A, Séminaires Méditerranéens, 42, 79–85.
Gunnaiah, R., Jagadeesha, R. C., Cholin, S., Prabhuling, G., Govindaswamy Babu, A., Fakrudin, B., ... & Murthy, S. B. (2021). Genetic diversity assessment and population structure analysis of pomegranate cultivars from different countries and Himalayan wild accessions. The Journal of Horticultural Science and Biotechnology, 96(5), 614-623. https://doi.org/10.1080/14620316.2021.1899854.
Kader, A.A. (2006). Postharvest biology and technology of pomegranates, In: Pomegranates. CRC Press, 229–238. ISBN: 9780429125539.
Kashyap, G. & Gautam, M.D. (2012). Analysis of vitamin c in commercial and naturals substances by iodometric titration found in nimar and malwaregeion. Journal of Scientific Research in Pharmacy, 1(2), 77–78.
Kulkarni, A.P. & Aradhya, S.M . (2005). Chemical changes and antioxidant activity in pomegranate arils during fruit development. Food Chemistry, 93(2), 319–324. https://doi.org/10.1016/j.foodchem.2004.09.029.
Martinez, J.J., Melgarejo, P., Hernández, F., Salazar, D.M. & Martinez, R. (2006). Seed characterisation of five new pomegranate (Punica granatum L.) varieties. Scientia Horticulturae, 110(3), 241–246. https://doi.org/10.1016/j.scienta.2006.07.018.
McCready, R.M., Guggolz, J., Silviera, V. & Owens, H.S. (1950). Determination of starch and amylose in vegetables. Analytical Chemistry, 22(9), 1156–1158.  https://doi.org/10.1021/ac60045a016.
Melgarejo-Sánchez, P., Martínez, J.J., Legua, P., Martínez, R., Hernández, F. & Melgarejo, P. (2015). Quality, antioxidant activity and total phenols of six Spanish pomegranates clones. Scientia Horticulturae, 182, 65–72. https://doi.org/10.1016/j.scienta.2014.11.020.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426–428. https://doi.org/10.1021/ac60147a030.
Mirjalili, S. A. (2002). Recognition of pomegranate. Agricultural Education Publication, Iran. 235 pages.
Mirjalili, S.A. (2016). Pomegranate: biodiversity and genetic resources, a review, Rostaniha, 17(1), 1–18. https://doi.org/10.22092/BOTANY.2016.106999.
Mirjalili, S.A., Qebouli, M., Bouazizi, A. & Aghajani, M. (2018). Study of biochemical diversity of phenolic compounds and anthocyanins in the fruit juice of Punica granatum L. among 25 genotypes of Malas cultivar. Ecophytochemistry of Medical Plants, 24(4),1-13. https://doi.org/20.1001.1.23223235.1397.6.4.1.3.
Mphahlele, R.R., Fawole, O.A., Mokwena, L.M. & Opara, U.L. (2016). Effect of extraction method on chemical, volatile composition and antioxidant properties of pomegranate juice, South African Journal of Botany, 103, 135–144. https://doi.org/10.1016/j.sajb.2015.09.015.
Omokolo Ndoumou, D., Tsala Ndzomo, G. & Djocgoue, P.F. (1996). Changes in carbohydrate, amino acid and phenol contents in cocoa pods from three clones after infection with phytophthora megakarya Bra. and Grif. Annals of Botany, 77(2), 153–158. https://doi.org/10.1006/anbo.1996.0017.
Opara, L.U., Al-Ani, M.R. & Al-Shuaibi, Y.S. (2009). Physico-chemical properties, vitamin C content, and antimicrobial properties of pomegranate fruit (Punica granatum L.), Food and Bioprocess Technology, 2(3), 315–321. https://doi.org/10.1007/s11947-008-0095-5.
Ozgen, M., Durgaç, C., Serçe, S. & Kaya, C. (2008). Chemical and antioxidant properties of pomegranate cultivars grown in the Mediterranean region of Turkey. Food Chemistry, 111(3), 703–706. https://doi.org/:10.1016/j.foodchem.2008.04.043.
Pinhas, S. & Elizer, E. (1996). Biology of citrus. Cambridge University Press, pp: 230.
Radunić, M., Jukić Špika, M., Goreta Ban, S., Gadže, J., Díaz-Pérez, J.C. & Maclean, D. (2015). Physical and chemical properties of pomegranate fruit accessions from Croatia. Food Chemistry, 177, 53–60. https://doi.org/ 10.1016/j.foodchem.2014.12.102.
Sarkhosh, A. Zamani, Z. Fattahi Moghadam, M. Ebadi, A. Saie, A. Tabatabaei, S. Z. & Akrami, M.R. (2006). Study of relationship among quality and quantity traits in some fruits of pomegranate genotypes. Journal of Science and Technology of Agriculture and Natural Resources, 4, 147–160.
Sarkhosh, A., Zamani, Z., Fatahi, R. & Ebadi, A. (2006). RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Scientia Horticulturae, 111(1), 24–29. https://doi.org/:10.1016/j.scienta.2006.07.033.
Sarkhosh, A., Zamani, Z., Fatahi, R. and Ranjbar, H. (2009). Evaluation of genetic diversity among Iranian soft-seed pomegranate accessions by fruit characteristics and RAPD markers. Scientia Horticulturae, 121(3), 313–319. https://doi.org/10.1016/j.scienta.2009.02.024.
Sarkhosh, A, Zamani, Z., Fattahi-Moghadam, M.R., Ebadi, A., Saie, A., Tabatabaei, S.Z. & Akrami, M.R. (2006). Study of relationship among quality and quantity traits in some fruits of pomegranate genotypes. Journal of Science and Technology of Agriculture and Natural Resources, 4, 146–185. https://doi.org/10.22059/ijhs.2017.129021.817.
Sarkhosh, ‌A. Zamani, ‌Z. Fatahi-Moghadam, M. Gogodi, ‌H. & Hadian, C. (2007). A review of medicinal and pharmacological properties of pomegranate, Quarterly Journal of Medicinal Plants, 22(2), 1-12. https://doi.org/ 20.1001.1.2717204.2007.6.22.2.5.
Sarkhosh, A.,  Yavari, A., &  Zamani, Z. (2021). The Pomegranate: Botany, Production and Uses, CABI, USA.
Sau, S., Sarkar, S., Mitra, M., & Gantait, S. (2021). Recent trends in agro-technology, post-harvest management and molecular characterisation of pomegranate. The Journal of Horticultural Science and Biotechnology, 96(4), 409-427. https://doi.org/10.1080/14620316.2021.1877201
Shahsavari, S., Noormohammadi, Z., Sheidai, M. & Vaifeshenas, MR. (2021). Genetic structure, clonality and diversity in commercial pomegranate (Punica granatum L.) cultivars. Genetic Resourses and Crop Evolution, 68, 2943–2957. https://doi.org/10.1007/s10722-021-01167-8.
Shulman, Y., Fainberstein, L. & Lavee, S. (1984). Pomegranate fruit development and maturation. Journal of Horticultural Science, 59(2), 265–274. https://doi.org/10.1080/00221589.1984.11515196.
Shwartz, E., Glazer, I., Bar-Ya’akov, I., Matityahu, I., Bar-Ilan, I., Holland, D. & Amir, R. (2009). Changes in chemical constituents during the maturation and ripening of two commercially important pomegranate accessions. Food Chemistry, 115(3), 965–973. https://doi.org/10.1016/j.foodchem.2009.01.036.
Singleton, V.L. & Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144–158.
Sun, T. & Ho, C.T. (2005). Antioxidant activities of buckwheat extracts. Food Chemistry, 90(4), 743–749. https://doi.org/10.1016/j.foodchem.2004.04.035.
Tatari, ‌ M. Qazvini, F., Qasemnejad, M. Mousavi, S. & Tabatabai, A. (2011). Morphological and biochemical characteristics of the fruit of some pomegranate cultivars in Saveh climatic conditions. ‌ Seed and Plant Journal, ‌ 27(1), 87-69 (In persian).
Tehranifar, A., Zarei, M., Nemati, Z., Esfandiyari, B. & Vazifeshenas, M.R. (2010) ‘Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae, 126(2), 180–185. https://doi.org/10.1016/j.scienta.2010.07.001
Tezcan, F., Gültekin-Özgüven, M., Diken, T., Özçelik, B. & Erim, F.B. (2009) Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices, Food Chemistry, 115(3), 873–877.
Usanmaz, S. (2014). Yield and Pomological Characteristics of Three Pomegranate (Punica granatum L) Cultivars: Wonderful, Acco and Herskovitz. American Journal of Agriculture and Forestry, 2(3), 61. https://doi.org/10.11648/j.ajaf.20140203.12.
Valero, C. (2000). Design guidelines for a quality assessment system of fresh fruits in fruit centers and hypermarkets. Agricultural Engineering International: CIGR Ejournal, II(August 2000).
Varasteh, F., Arzani, K., Zamani, Z. & Mohseni, A. (2009). Evaluation of the most important fruit characteristics of some commercial pomegranate (Punica granatum L.) cultivars grown in Iran’, Acta Horticulturae, 818, 103–108. https://doi.org/10.17660/ActaHortic.2009.818.13.
van Handel, E. (1968). Direct microdetermination of sucrose. Analytical Biochemistry, 22(2), 280–283. https://doi.org/10.1016/0003-2697(68)90317-5.
Wills, R.H.H. (1981). Postharvest, an introduction to the physiology and handling of fruits and vegetables. https://doi.org/10.1079/9781786391483.0000.
Zahravy, M., & Vazifeshenas, M.R. (2017). Study of genetic diversity in pomegranate of Yazd collection Province of Iran. Iranian Journal of Genetics and Plant Breeding, 6, 2 20-35. https://doi.org/10.30479/IJGPB.2017.1488.
 Zamani, Z., Sarkhosh, A., Fatahi, R. & Ebadi, A. (2007). Genetic relationships among pomegranate genotypes studied by fruit characteristics and RAPD markers. The Journal of Horticultural Science and Biotechnology, 82(1), 11–18. https://doi.org/10.1080/14620316.2007.11512192.
Zarei, M., Azizi, M. & Bashir-Sadr, Z. (2011). Evaluation of physicochemical characteristics of pomegranate (Punica granatum L.) fruit during ripening. Fruits, 66(2), 121–129. https://doi.org/10.1051/fruits/2011021.
 Zarei, M. & Azizi, M. (2010). Evaluation of some physical and chemical properties of six Iranian pomegranate fruit cultivars at ripening stage. Journal of Horticultural Sciences, 24(2), 182-175. https://doi.org/ 10.22067/JHORTS4.V1389I2.7995.