Document Type : Original Article
Authors
Department of Plant Production, Soil Science and Agricultural Engineering, University of Limpopo – Turfloop Campus, Private Bag X1106, Sovenga 0727, Limpopo Province, South Africa
Abstract
Purpose: This study investigated the effect of harvest time, postharvest dehydration + waxing and storage temperature on rind- free and conjugated phenolics and their ability to alleviate chilling injury and pitting of ‘Benny’ Valencia oranges during cold storage. Research method: Fruit were harvested at early, mid- and late season, and thereafter, divided into control, dehydrated, waxed + dehydrated portions. After treatment, fruit were stored at -0.6 and 4.5°C for 28 days, thereafter, 7 days at ambient temperature (25°C). Findings: In general, peel pitting index (PPI) was significantly higher for late season fruit, while, CI was higher for early season fruit, especially at -0.6°C storage. Furthermore, dehydration stress without waxing resulted in significantly higher PPI and CI at -0.6°C when compared with 4.5°C storage. With respect to both free and soluble conjugated phenolics, the control fruit showed higher levels of rind phenolics, especially at late harvest across all the storage temperatures. Therefore, untreated fruit appeared to tolerate cold stress by up-regulating endogenous systems of total rind phenolics. Postharvest dehydration repressed endogenous phenolics synthesis. In conclusion, susceptibility to pitting disorder increases with harvest time, dehydration stress, while fruit harvested early were highly susceptible to CI. Research limitations: The main limitation of this study is the lack of specific phenolics. Originality/Value: The study found that dehydration plus waxing has a significant effect on chilling and non-chilling citrus ‘Benny’ Valencia fruit. Furthermore, these treatments induced an increase in rind total phenolics to mitigate rind physiological disorders during extended cold storage.
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Abeysinghe, D. C., LI, X., Sun, C. D., Zhang, W. S., Zhou, C. H., & Chen, K. S. (2007). Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chemistry, 104, 1338-1344. https://doi.org/10.1016/j.foodchem.2017.01.047.
Agustí, M., Almela, V., Juan, M., Alférez, F., Tadeo, F., & Zacarías, L. (2001). Histological and physiological characterization of rind breakdown of ‘Navelate’ sweet orange. Annals of Botany, 88, 415-422. https://doi.org/10.1006/anbo.2001.1482.
Alférez, F., & Burns J.K. (2004). Postharvest peel pitting at non-chilling temperatures in grapefruit is promoted by changes from low to high relative humidity during storage. Postharvest Biology and Technology, 32, 79-87. https://doi.org/10.1016/j.postharvbio.2003.09.018.
Alférez, F., & Zacarías, L. (2000). Postharvest pitting in navel oranges at non-chilling temperature: influence of relative humidity. Acta Horticulturae, 553, 307-308. https://doi.org/10.17660/ActaHortic.2001.553.74.
Alférez, F., & Zacarías, L. (2014). Influence of fruit maturity in the susceptibility of Navelina oranges to develop postharvest non-chilling peel pitting. Revista de Agaroquimica Tecnologia de Alimentos, 20, 183-191. https://doi.org/10.1177/1082013213476077.
Alférez, F., Agustí, M., & Zacarías, L. (2003). Postharvest rind staining in Navel oranges is aggravated by changes in storage relative humidity: Effect on respiration, ethylene production and water potential. Postharvest Biology and Technology, 28, 143-152. https://doi.org/10.1016/S0925-5214(02)00120-5.
Alférez, F., Alquezar, B., Burns, J.K., & Zacarías, L. (2010). Variation in water, osmotic and turgor potential in peel of ‘Marsh’ grapefruit during development of postharvest peel pitting. Postharvest Biology and Technology, 56, 44-49. https://doi.org/10.1016/j.postharvbio.2009.12.007.
Alquezar, B., Mesero, C., Alférez, F., Agustí, M., & Zacarías, L. (2010). Morphological and ultrastructural changes in the peel of ‘Navelate’ oranges in relation to variations in relative humidity during postharvest storage and development of peel pitting. Postharvest Biology and Technology, 56, 163-170. https://doi.org/10.1016/j.postharvbio.2009.12.005.
Bajji, M., Kinet, J.M., & Lutts, S. (2002). The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regulation, 36, 61-70. https://doi.org/10.1023/A:1014732714549.
Baldwin, E. A., Burns, J. K., Kazokas, W., Brecht, J. K., Hagenmaier, R. D., Bender, R. J., & Pesis, E. (1999). Effect of two edible coatings with different permeability characteristics on mango (Mangifera indica L.) ripening during storage. Postharvest Biology and Technology, 17, 215-226. https://doi.org/10.1016/S0925-5214(99)00053-8.
Chaudhary, P. R., Yu, X., Jayaprakasha, G. K., & Patil. B. S. (2016). Influence of storage temperature and low-temperature conditioning on the levels of health-promoting compounds in Rio Red grapefruit. Food Science Nutrition, 5, 545-553. https://doi.org/10.1002/fsn3.429.
Citrus Growers Association of Southern Africa (CGA). (2015). Key industry statistics for citrus growers 2015. Citrus Growers Association of Southern Africa Annual Report, 2015.
Cohen, E., Shapiro, B., Shalom, Y., & Klein, J.D. (1994). Water loss: a non-destructive indicator of enhanced cell membrane permeability of chilling-injured citrus fruits. Journal of the American Society of Horticultural Science, 119, 983-986. https://doi.org/10.21273/JASHS.119.5.983.
Corona, O., Planeta, D., Bambina, P., Giacosa, S., Paissoni, M.A., Squadrito, M., Torchio, F., Segade, S.R., Cinquanta, L., Gerbi, V., & Rolle. (2020). Influence of different dehydration levels on volatile profile, phenolics contents and skin hardness of alkaline pre-treated grapes cv Muscat of Alexandria (Vitis vinifera L.). Foods, 9(5), 666. https://doi.org/10.3390/foods9050666.
Cronje, P.J.R., Ehlers, J.L., Alférez, F., & Theron, K.I. (2017). Efficacy of pre-harvest sprays to reduce rind pitting on Benny Valencia orange. Citrus Research and Technology, 38(2), 160-168. http://doi.org/10.4322/crt.ICC028.
Cronjé, P.RJ., Zacarías, L., & Alférez, F. (2017). Susceptibility to postharvest peel pitting in Citrus fruits as related to albedo thickness, water loss and phospholipase activity. Postharvest Biology and Technology, 123, 77-82. https://doi.org/10.1016/j.postharvbio.2016.08.012.
Davis, F.S., & Albrigo L.G. (1994). Citrus. In: Crop Production Science in Horticulture Series. CAB International, Wallington, p.134-135.
Dodd, M., Cronje, P., Taylor, M., Huysamer, M., Kruger, F., Lotz, E., & Van der Merwe, K. (2010). A review of the postharvest handling of fruits in South Africa over the past twenty-five years. South African Journal of Plant and Soil, 27, 97-116. https://doi.org/10.1080/02571862.2010.10639974.
Dou, H. (2004). Effect of coating application on chilling injury of grapefruit cultivars. Horticultural Science, 39(3), 558-561. https://doi.org/10.21273/HORTSCI.39.3.558.
Dou, H. (2005). The influence of harvesting time and geographical location susceptibility to physiological peel disorders associated with four Florida grapefruit cultivars. Journal of Horticultural Science and Biotechnology, 80, 466-470. https://doi.org/10.1080/14620316.2005.11511961.
Ehlers, J.L. (2016). Post-harvest rind pitting studies on ‘Valencia’ orange. MSc dissertation, University of Stellenbosch, South Africa.
Fawole, O.A., & Opara, U.L. (2013). Effect of storage temperature and duration on physiological responses of pomegranate fruit. Industrial Crops and Products, 47, 300-308. https://doi.org/10.1016/j.indcrop.2013.03.028.
Germano, T.A., Aguiar, R.P., Bastos, M.S.R., & Moreira, R. (2019). Galactomannan-carnauba wax coating improves the antioxidant status and reduces chilling of ‘Paluma’ guava. Postharvest Biology and Technology, 149(5), 9-17. http://doi:10.1016/j.postharvbio.2018.11.013.
Gonzalez-Aguilar, G.A., Zacarías, L., Perez-Amador, M.A., Carbonell, J., & Lafuente, M.T. (2000). Polyamine content and chilling susceptibility are affected by seasonal changes in temperature and by conditioning temperature in cold stored ‘Fortune’ mandarins. Physiologia Plantarum, 108, 140-146. https://doi.org/10.1034/j.1399-3054.2000.108002140.x.
Hordijk, J. (2013). Studies to reduce the incidence of chilling injury in Navel orange fruit. MSc dissertation, University Stellenbosch, South Africa.
Hu, H., Li, X., Dong, C., & Chen, W. (2011). Effects of wax treatment on quality and postharvest physiology of pineapple fruit in cold storage. African Journal of Biotechnology, 10, 7592-7603. https://doi.org/10.5897/AJB10.2474.
Huang, R., Xia, R., Hu, L., Lu, L., & Wang, M., (2007). Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation. Scientia Horticulturae, 113(2), 116-172. https://doi.org/10.1016/j.scienta.2007.03.010.
Joubert, J. (2016). Influence of rind water content on mandarin citrus fruit quality. MSc dissertation, University of Stellenbosch, South Africa.
Khumalo, N.P. (2006). Factors affecting post-storage quality of ‘Nules Clementine’ mandarin fruit with special reference to rind breakdown. MSc dissertation, University of Stellenbosch, South Africa.
Ladaniya, M.S. (2008). Citrus fruit: Biology, Technology and Evaluation. 1st edition, New York.
Lado, J., Cronje, P.J.R., Rodrigo, M.J., & Zacaría, L. (2019). Citrus. In Postharvest Physiology Disorders of Fruits and Vegetables. de Freitas, S.T., Pareek, S., Eds: CRC Press: Boca Raton, FL, USA.
Lafuente, M.T., & Sala, J, M. (2002). Abscisic acid levels and the influence of ethylene, humidity and storage temperature on the incidence of postharvest rindstaining of ‘Navelina’ orange (Citrus sinensis L. Osbeck) fruit. Postharvest Biology and Technology, 25, 49-57. https://doi.org/10.1016/S0925-5214(01)00162-4.
Lafuente, M.T., Martínez-Téllz, M.A., & Zacarías, L. (1997). Abscisic acid in the response of ‘Fortune’ mandarins to chilling. Effect of maturity and high-temperature conditioning. Journal of the Science of Food and Agriculture, 73(4), 494-502. https://doi.org/10.1002/(SICI)1097-0010(199704)73:4<494: AID-JSFA761>3.0.CO;2-B.
Li, L., Sun, J., & Gao, H. (2017). Effects of polysaccharide-based edible coatings on quality and antioxidant enzyme system of strawberry during cold storage. International Journal of Polymer Science, 56, 1-8. https://doi.org/10.1155/2017/9746174.
Liang, S., Kuang, J., Ji, S., Chen, Q., Deng, W., Min, T., Shan, W., Chen, J., & Lu, W. (2020). The membrane lipid metabolism in horticultural products suffering chilling injury. Food Quality and Safety, 4(1), 9-14. https://doi.org/10.1093/fqsafe/fyaa001.
Magwaza, L.S. (2008). A preliminary study on the effect of climatic conditions and fruit mineral concentration on the development of lenticel damage in ‘Tommy Atkins’ and ‘Keitt’ mangos and rind pitting in ‘Benny’ Valencia citrus fruits. MSc thesis, University of Kwazulu-Natal, South Africa.
Magwaza, L.S., Opara, U.L., Cronjé, P.J., Landahl, S., & Terry, L.A. (2013). Non-chilling physiological rind disorders in citrus fruit. Horticultural Reviews, 41, 131-176. https://doi.org/10.1002/9781118707418.ch03.
Maina, B., Ambuko, J., Hutchinson, M.J., & Owino, W.O. (2019). The effect of waxing options on shelf life and postharvest quality of “Ngowe” mango fruits under different storage conditions. Advances in Agriculture, 1-9. https://doi.org/10.1155/2019/5085636.
Mathaba, N. (2012). The cascade of physiological events leading to chilling injury: The effect of post-harvest hot water and molybdenum applications to lemon (Citrus limon) fruit. PhD thesis, University of KwaZulu-Natal, South Africa.
Mditshwa, A. (2012). The potential of postharvest potassium silicate dips to mitigate chilling injury on citrus fruit. MSc thesis, University of KwaZulu-Natal, South Africa.
Medeira, M.C., Maia, M.I., & Vitor, R.F. (1999). The first stages of pre-harvest ‘peel pitting’ development in ‘Encore’ mandarin. A histological and ultrastructural study. Annals of Botany, 83, 667-673. https://doi.org/10.1006/anbo.1999.0867.
Meighani, H., Ghasemnezhad, M., & Bakhshi, D, (2015). Effect of different coatings on post-harvest quality and bioactive compounds of pomegranate (Punica granatum L.) fruits. Journal of Food Science and Technology, 52, 4507-4514. https://doi.org/10.1007/s13197-014-1484-6.
Moggia, C., Sepúlveda, D., Valdé, M., Araya, C.M., Pacheco, P., Diaz, V., Lobos, G.A., Beaudry, R., & Lorca-Alvarez, J. (2023). Dangler for accelerated dehydration: A novel system for assessing the impacts of relative humidity on fruit water loss during cold storage of blueberries. HortScience, 58(7), 717-721. https://doi.org/10.21273/HORTSCI17016-22.
Moon, D.G., Cho, Y.S., Mizutani, F., Rutto, K.L., & Bhusal, R.C. (2003). Wax deposition on the fruit surface of Satsuma Mandarin as affected by water stress, Asian Journal of Plant Sciences, 2, 1138-1141. https://doi.org/10.3923/ajps.2003.1138.1141.
Mothapo, M.J., Mafeo, T.P., & Mathaba, N. (2022). Expression of genes associated with ‘Benny’ Valencia rind pitting. Acta Horticulturae, 1336, 263-270. https://doi.org/10.17660/ActaHortic.2022.1336.35.
Mothapo, M.J., Mathaba, N., Mafeo, T.P., & Mphahlele, R.R. (2018). Effect of production site and postharvest treatments on the incidence of rind pitting and chemical properties of 'Benny' sweet orange. Acta Horticulturea, 1201, 107-114. https://doi.org/10.17660/ActaHortic.2018.1201.15.
Negi, P.S., & Roy, S.K. (2000). Effect of low‐cost storage and packaging on quality and nutritive value of fresh and dehydrated carrots. Journal of the Science of Food and Agriculture, 80, 2169-2175. https://doi.org/10.1002/1097-0010(200012)80:15<2169: AID-JSFA762>3.0.CO;2-J.
Olarewaju, O.O., Magwaza, L.S., Fawole, O.A., Tesfay, S.Z., & Opara, U.L. (2020). Canopy position affect rind biochemical properties of ‘Marsh’ grapefruit during postharvest cold storage at non-chilling temperature. International Journal of Fruit Science, 20, S894-S909. https://doi.org/10.1080/15538362.2020.1772182.
Orr, G.R., & Raison, J.K. (1990). Phase transitions in liposomes formed from the polar lipids of mitochondria from chilling-sensitive plants. Plant Physiology, 81, 807-811. https://doi.org/10.1104/pp.81.3.807.
Pailly, O., Tison, G., & Amouroux, A. (2004). Harvest time and storage conditions of ‘Star Ruby’ grapefruit (Citrus paradisi Macf.) for short distance summer consumption, Postharvest Biology and Technology, 34, 65-73. https://doi.org/10.1016/j.postharvbio.2004.04.005.
Petriccione, M. Mastrobuoni, F., & Pasquariello M. S. (2015). Effect of chitosan coating on the postharvest quality and antioxidant enzyme system response of strawberry fruit during cold storage. Journal of Foods, 4, 501-523. https://doi.org/10.3390/foods4040501.
Sala, J.M. (1998). Involvement of oxidative stress in chilling injury in cold-stored mandarin fruits. Postharvest Biology and Technology, 13, 255-261. https://doi.org/10.1016/S0925-5214(98)00011-8.
Saltveit, M.E. (2000). Discovery of chilling injury. In: Kung, S.D., Yang, S.F. (Editions), Discoveries in Plant Biology, 3, 690-697.
Sang, N., & Hai, L.H. (2020), Effect of ratio of bees wax and carnauba wax in mixed wax on respiration rate, weight loss, fruit decay and chemical quality of Vietnamese passion fruits during low temperature storage. Pakistan Journal of Biotechnology, 17(2), 63-70. http://doi.org/10.34016/pjbt.2020.17.2.63.
Seymour, G. B., Taylor, J. E., & Tucker, A. (1996). Biochemistry of Fruit Ripening. Chapman and Hall, UK, p. 151-187.
Shin, Y., Ryu, J.A., Liu, R.H., Nock, J.F., & Watkins, C.B. (2008). Harvest maturity, storage temperature and relative humidity affect fruit quality, antioxidant contents and activity, and inhibition of cell proliferation of strawberry fruit. Postharvest Biology and Technology, 49, 201-209. https://doi.org/10.1016/j.postharvbio.2008.02.008.
Storey, R., and Treeby, M.T., (1994). The morphology of epicuticular wax and albedo cells of orange fruit in relation to albedo breakdown. Journal of Horticultural Science, 69, 329-338. https://doi.org/10.1080/14620316.1994.11516462.
Strano, M.C., Altieri, G., Allegra, M., Di Renzo, G.C., Paterna, G., Matera, A., & Genovese, F. (2022). Postharvest technologies of fresh citrus fruit: Advances and recent developments for the loss reduction during handling and storage. Horticulturae, 8(7), 612. https://doi.org/10.3390/horticulturae8070612.
Tavarini, S., Degl’Innocenti, E., Remorini, D., Massia, R., & Guidi, L. (2008). Antioxidant capacity, ascorbic acid, total phenols and carotenoids changes during harvest and after storage of Hayward kiwifruit. Food Chemistry, 107(1), 282-288. https://doi.org/10.1016/j.foodchem.2007.08.015
Tzoutzoukou, G. G., & Bouranis, D. C. (1997). Effect of prepares application of calcium on postharvest physiology of apricot fruit. Journal of Plant Nutrition, 20, 295-309. https://doi.org/10.1007/s13197-014-1288-8.
Undurraga, P.L., Olaeta, J.A., Retamales, J.B., & Toso, A.M. (2014). Manifestation of peteca and fruit quality loss in Eureka lemon under different cold storage conditions. Agrociencia, 41(2), 133-139.
Vercher R., Tadeo, F.R., Almela, V., Zaragoza, S., Primo-Millo, E., & Agustí M. (1994). Rind structure, epicuticular wax morphology and water permeability of ‘Fortune’ mandarin fruits affected by peel pitting. Annual Botany, 74, 619-625. https://doi.org/10.1006/anbo.1994.1163.
Wang, C.Y. (1993). Approaches to reduce chilling injury of fruits and vegetables. Horticultural Reviews, 15, 63-95. https://doi.org/10.1002/9780470650547.ch2.
Wang, S.Y., & Lin, S.H. (2000). Antioxidant activity in fruit and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry, 48, 140-146. https://doi.org/10.1021/jf9908345.
Wild, B.L. (1993). Reduction of chilling injury in grapefruit and oranges stored at 1°C by pre-storage hot dip treatments, curing, and wax application. Australian Journal of Experimental Agriculture, 33(4), 495-498. https://doi.org/10.1071/EA9930495.
Yaman, O., & Bayoindurh, L. (2022). Effects of an edible coating and cold storage on shelf life and quality of cherries. LWT-Food science and Technology, 35, 146-150. https://doi.org/10.1006/fstl.2001.0827.
Yuan, G., Sun, B., Yuan, J., & Wang, Q. (2010). Effect of 1-methylcyclopropene on shelf-life, visual quality, antioxidant enzymes and health-promoting compounds in broccoli florets. Food Chemistry, 118, 774-781. https://doi.org/10.1016/j.foodchem.2009.05.062.
Zhou, R., Li, Y., Yan, L., & Xie, J. (2011). Effect of edible coatings on enzymes, cell-membrane integrity, and cell-wall constituents in relation to brittleness and firmness of Huanghua pears (Pyrus pyrifolia Nakai, cv. Huanghua) during storage. Food Chemistry, 124, 569-575. https://doi.org/10.1016/j.foodchem.2010.06.075.
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