Document Type : Original Article
Authors
1 Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran
2 Department of Biosystems Engineering, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Purpose: Lemon (Citrus limon) is a highly important citrus species worldwide. However, its semi-tropical nature makes it susceptible to chilling, extensive research on postharvest treatments to preserve its quality under low temperatures. Research Method: The treatments included 500 μM melatonin (M), 50 μM methyl jasmonate (J), and 5 mM gamma-aminobutyric acid (GABA). Lemons stored at 3 ± 1°C and a relative humidity of 85-95% for 100 days plus 5-day shelf life. Findings: The results revealed that most of the experimental treatments, except for the combination of GABA + J and GABA + J + M, significantly reduced fruit weight loss. Notably, the melatonin treatment showed a 22.7% lower weight loss compared to the control fruits. Furthermore, the melatonin treatment exhibited the highest fruit firmness (49 N), while the control treatment had the lowest (36.3 N). Regarding the quality parameters, individual treatments, and the GABA + M treatment resulted in significantly higher total soluble solids (TSS) and a lower TSS/TA compared to the control at the end of the storage period. Except for the M + GABA and M + J treatments, all other treatments showed higher ascorbic acid content compared to the control. Additionally, the melatonin treatment showed significant differences in various color indices compared to the control. Research limitations: There was no limitation. Originality/Value: Overall, fruits treated with M, J, GABA, and GABA + M demonstrated higher marketability compared to the control and other treatments. Consequently, it is recommended to utilize these treatments individually rather than in combination to maintain the quality of lemon fruits.
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Akan, S., Tuna Gunes, N., & Yanmaz, R. (2019). Methyl jasmonate and low temperature can help for keeping some physicochemical quality parameters in garlic (Allium sativum L.) cloves. Food Chemistry, 270, 546–553. https://doi.org/https://doi.org/10.1016/j.foodchem.2018.07.085
AOAC. (2000). Vitamins and other nutrients, official methods of analysis (17th ed.). Washington, D.C.: AOAC International16–20.
Asgarian, Z.S., Karimi, R., Ghabooli, M., & Maleki, M. (2022). Biochemical changes and quality characterization of cold-stored ‘Sahebi’grape in response to postharvest application of GABA. Food Chemistry, 373, 131401. https://doi.org/10.1016/j.foodchem.2021.131401
Baloyi, R. G., Mafeo, T. P., & Mathaba, N. (2023). Effect of pre-and post-harvest factors on ‘Benny’ Valencia fruit rind phenolics on mitigation of chilling and non-chilling disorders during cold storage. Journal of Horticulture and Postharvest Research, 6(3), 299-316. https://doi.org/10.22077/jhpr.2023.6387.1319
Baswal, A.K., Dhaliwal, H.S., Singh, Z., Mahajan, B.V.C., & Gill, K.S. (2020). Postharvest application of methyl jasmonate, 1-methylcyclopropene and salicylic acid extends the cold storage life and maintain the quality of ‘Kinnow’ mandarin (Citrus nobilis L. X C. deliciosa L.) fruit. Postharvest Biology and Technology, 161, 111064. https://doi.org/10.1016/j.postharvbio.2019.111064
Fan, Z., Lin, B., Lin, Y., Chen, Yazhen., Chen, G., Chen, J., Wang, H., Chen, Yihui., & Lin, H. (2024). Alleviation of chilling injury in cold-stored Chinese olive (Canarium album Lour.) fruit by γ-aminobutyric acid treatment in relation to ROS metabolism. Scientia Horticulturae, 327, 112851. https://doi.org/https://doi.org/10.1016/j.scienta.2024.112851
Ghorbani, B., Pakkish, Z., & Khezri, M. (2018). Nitric oxide increases antioxidant enzyme activity and reduces chilling injury in orange fruit during storage. New Zealand Journal of Crop and Horticultural Science, 46(2): 101-116. https://doi.org/10.1080/01140671.2017.1345764
Habibi, F., Ramezanian, A., Guillén, F., Serrano, M., & Valero, D. (2020). Blood oranges maintain bioactive compounds and nutritional quality by postharvest treatments with γ-aminobutyric acid, methyl jasmonate or methyl salicylate during cold storage. Food Chemistry, 306, 125634. https://doi.org/10.1016/j.foodchem.2019.125634
Habibi, F., Ramezanian, A., Rahemi, M., Eshghi, S., Guillén, F., Serrano, M., & Valero, D. (2019). Postharvest treatments with γ‐aminobutyric acid, methyl jasmonate, or methyl salicylate enhance chilling tolerance of blood orange fruit at prolonged cold storage. Journal of the Science of Food and Agriculture, 99, 6408–6417. https://doi.org/10.1002/jsfa.9920
Huang, R.H., Liu, J.H., Lu, Y.M., & Xia, R.X. (2008). Effect of salicylic acid on the antioxidant system in the pulp of ‘Cara Cara’ navel orange (Citrus sinensis L. Osbeck) at different storage temperatures. Postharvest Biology and Technology, 47, 168–175. https://doi.org/10.1016/j.postharvbio.2007.06.018
Hui, G., Zheng, K.Z., Hong, K.C., Ni, C., Yue, Y., Dan, N.W., & Wei, C. (2016). Melatonin treatment delays postharvest senescence and regulates reactive oxygen species metabolism in peach fruit. Postharvest Biology and Technology, 118, 103–110. https://doi.org/10.1016/j.postharvbio.2016.03.006
Jannatizadeh, A. (2019). Exogenous melatonin applying confers chilling tolerance in pomegranate fruit during cold storage. Scientia Horticulturae, 246, 544–549. https://doi.org/10.1016/j.scienta.2018.11.027
Jimenez, A., Creissen, G., Kular, B., Firmin J., Robinson, S., Verhoeyen, M., & Mullineaux, P. (2002). Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening. Planta, 214, 751–758.
Khorshidi, Sh., Davarynejad, Gh., Tehranifar, A., & Fallahi, E. (2011). Effect of modified atmosphere packaging on chemical composition, antioxidant activity, anthocyanin, and total phenolic content of cherry fruits. Horticulture and Environment Biotechnology, 52, 471-481.
Lado, J., Gambetta, G., & Zacarias, L. (2018). Key determinants of citrus fruit quality: Metabolites and main changes during maturation. Scientia Horticulturae, 233, 238-248. https://doi.org/10.1016/j.scienta.2018.01.055
Lara, I., Belge, B., & Goulao, L.F. (2014). The fruit cuticle as a modulator of postharvest quality. Postharvest Biology and Technology, 87, 103–112. https://doi.org/10.1016/j.postharvbio.2013.08.012
Liao, L., Li, J., Lan, X., Li, Yaman, Li, Yunjie, Huang, Z., Jin, Z., Yang, Y., Wang, X., Zhang, M., Sun, G., Zhang, X., Xiong, B., & Wang, Z. (2024). Exogenous melatonin and interstock treatments confer chilling tolerance in citrus fruit during cold storage. Scientia Horticulturae, 327, 112802. https://doi.org/https://doi.org/10.1016/j.scienta.2023.112802
Liu, S., Huang, H., Huber, D.J., Pan, Y., Shi, X., & Zhang, Z. (2020). Delay of ripening and softening in ‘Guifei’mango fruit by postharvest application of melatonin. Postharvest Biology and Technology, 163, 111136. https://doi.org/10.1016/j.postharvbio.2020.111136
Lorente-Mento, J. M., Guillén, F., Martínez-Romero, D., Carrión-Antoli, A., Valero, D., & Serrano, M. (2023). γ-Aminobutyric acid treatments of pomegranate trees increase crop yield and fruit quality at harvest. Scientia Horticulturae, 309, 111633. https://doi.org/10.1016/j.scienta.2022.111633
Ma, Q., Lin, X., Wei, Q., Yang, X., Zhang, Y., & Chen, J. (2021). Melatonin treatment delays postharvest senescence and maintains the organoleptic quality of ‘Newhall’navel orange (Citrus sinensis (L.) Osbeck) by inhibiting respiration and enhancing antioxidant capacity. Scientia Horticulturae, 286, 110236. https://doi.org/10.1016/j.scienta.2021.110236
Makule, E., Dimoso, N., & Tassou, S.A. (2022). Precooling and cold storage methods for fruits and vegetables in Sub-Saharan Africa: A Review. Horticulturae, 8(9), 776. https://doi.org/10.3390/horticulturae8090776
Mukhim, C., Nath, A., Deka, B.C., & Swer, T.L. (2015). Changes in physico-chemical properties of Assam lemon (Citrus limon Burm.) at different stages of fruit growth and development. Bioscan, 10(2), 535–537.
Ngaffo Mekontso, F., Duan, W., Cisse, E.H.M., Chen, T., & Xu, X. (2021). Alleviation of postharvest chilling injury of carambola fruit by γ-aminobutyric acid: Physiological, biochemical, and structural characterization. Frontiers in Nutrition, 891. https://doi.org/10.3389/fnut.2021.752583
Ramesh, S.A., Tyerman, S.D., Xu, B., Bose, J., Kaur, S., Conn, V., Domingos, P., Ullah, S., Wege, S., & Shabala, S. (2015). GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters. Nature communications, 6, 78-79.
Rapisarda, P., Bellomo, S.E., & Intelisano, S. (2001). Storage temperature effects on blood orange fruit quality. Journal of Agricultural and Food Chemistry, 49, 3230–3235. https://doi.org/10.1021/jf010032l
Rasouli, M., Saba, M.K., & Ramezanian, A. (2019). Inhibitory effect of salicylic acid and Aloe vera gel edible coating on microbial load and chilling injury of orange fruit. Scientia Horticulturae, 247, 27-34. https://doi.org/10.1016/j.scienta.2018.12.004
Rastegar, S., Khankahdani, H.H., & Rahimzadeh, M. (2020). Effects of melatonin treatment on the biochemical changes and antioxidant enzyme activity of mango fruit during storage. Scientia Horticulturae, 259, 108835. https://doi.org/10.1016/j.scienta.2019.108835
Rey, F., Zacarías, L., & Rodrigo, M.J. (2020). Carotenoids, vitamin C, and antioxidant capacity in the peel of mandarin fruit in relation to the susceptibility to chilling injury during postharvest cold Storage. Antioxidants, 9(12), 1296. https://doi.org/10.3390/antiox9121296
Sayyari, M., Babalar, M., Kalantari, S., Martínez-Romero, D., Guillén, F., Serrano, M., & Valero, D. (2011). Vapour treatments with methyl salicylate or methyl jasmonate alleviated chilling injury and enhanced antioxidant potential during postharvest storage of pomegranates. Food Chemistry, 124, 964–970. https://doi.org/10.1016/j.foodchem.2010.07.036
Serna-Escolano, V., Martínez-Romero, D., Giménez, M. J., Serrano, M., García-Martínez, S., Valero, D., & Zapata, P. J. (2021). Enhancing antioxidant systems by preharvest treatments with methyl jasmonate and salicylic acid leads to maintain lemon quality during cold storage. Food Chemistry, 338, 128044. https://doi.org/10.1016/j.foodchem.2020.128044
Siboza, X.I., Bertling, I., & Odindo, A.O. (2014). Salicylic acid and methyl jasmonate improve chilling tolerance in cold-stored lemon fruit (Citrus limon). Journal of Plant Physiology, 171, 1722–1731. https://doi.org/10.1016/j.jplph.2014.05.012
Sun, Y., Singh, Z., Tokala, V.Y., & Heather, B. (2019). Harvest maturity stage and cold storage period influence lemon fruit quality. Scientia Horticulturae, 249, 322–328. https://doi.org/10.1016/j.scienta.2019.01.056
Tarancón, P., Tárrega, A., González, M., & Besada, C. (2021). External quality of mandarins: influence of fruit appearance characteristics on consumer choice. Foods, 10(9), 2188. https://doi.org/10.3390/foods10092188
Vidal, A., Talens, P., Prats-Montalba´n, J.M., Cubero, S., Albert, F., & Blasco, J. (2013). In-line estimation of the standard colour index of citrus fruits using a computer vision system developed for a mobile platform. Food Bioprocess Technology, 6, 3412–3419.
Wang, S.-Y., Shi, X.-C., Liu, F.-Q., & Laborda, P. (2021). Effects of exogenous methyl jasmonate on quality and preservation of postharvest fruits: A review. Food Chemistry, 353, 129482. https://doi.org/10.1016/j.foodchem.2021.129482
Wu, C., Hao, W., Yan, L., Zhang, H., Zhang, J., Liu, C., & Zheng, L. (2023). Postharvest melatonin treatment enhanced antioxidant activity and promoted GABA biosynthesis in yellow-flesh peach. Food Chemistry, 419, 136088. https://doi.org/10.1016/j.foodchem.2023.136088
Yılmaz, M. (2024). Enhancing cold storage quality of blueberry (Vaccinium corymbosum ‘Bluecrop’) with methyl jasmonate treatments. Applied Fruit Science, 66, 81–88. https://doi.org/10.1007/s10341-023-01020-5
Ze, Y., Gao, H., Li, T., Yang, B., & Jiang, Y. (2021). Insights into the roles of melatonin in maintaining quality and extending shelf life of postharvest fruits. Trends in Food Science & Technology, 109, 569–578. https://doi.org/10.1016/j.tifs.2021.01.051
Zhang, Y., Tang, H., Lei, D., Zhao, B., Zhou, X., Yao, W., & Zhang, Y. (2023). Exogenous melatonin maintains postharvest quality in kiwiberry fruit by regulating sugar metabolism during cold storage. LWT, 174, 114385. https://doi.org/https://doi.org/10.1016/j.lwt.2022.114385
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