Document Type : Original Article

Authors

1 School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, P Bag X01 Scottsville 3209, Pietermaritzburg, South Africa

2 Department of Horticulture, Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa

Abstract

Purpose: The study was conducted to evaluate growth, physiological, morphological and yield response of gem squash plants following soil drench application of different plant extracts. Research method: A pot experiment conducted in the glasshouse was laid out following complete randomized design (CRD), with five replications. Thirty healthy, similar-sized gem squash plants were grown and treated with different treatments (plant extracts). Treatments included: Ascophyllum nodosum extract (ANE), aloe vera leaf extract (ALvE), garlic bulb extract (GBE), ginger rhizome extract (GRE), moringa leaf extract (MLE) and the control (no application). Findings: The soil drench application of plant extracts, especially ANE and MLE, had the best growth response of gem squash plants compared with other treatments and the control. Plants treated with ANE and MLE produced a greater number of leaves and branches and simultaneously produced broader leaf area compared to other plant extracts and the control. ANE-treated plants produced the highest leaf chlorophyll concentration, followed by ALvE and MLE. All plant extracts, ANE, MLE, ALvE and GBE, significantly increased the total dry biomass, except GRE was not significantly different from the control. The yield parameters, viz. total fruit yield, fruit mass and fruit diameter, were positively affected by all treatments applied, although ANE- and MLE-treated plants yielded the largest number of fruit/plants, heaviest fruit and biggest fruit compared to other treatments. Research limitations: There were no limitations identified. Originality/Value: Although further studies on plant extracts usage are still required, this study highlight the potential of plant extracts, especially ANE and MLE, as a natural biostimulants to improve growth and yield attributes of gem squash has been demonstrated.

Keywords

Main Subjects

Abd El-Gawad, H., & Osman, H. S. (2014). Effect of exogenous application of boric acid and seaweed extract on growth, biochemical content and yield of eggplant. Journal of Horticultural Science & Ornamental Plants, 6(3), 133-143.
Abdel-Mawgoud, A. M. R., Tantaway, A. S., Hafez, M. M., & Habib, H. A. M. (2010). Seaweed extract improves growth, yield and quality of different watermelon hybrids. Research Journal of Agriculture and Biological Sciences, 6(2), 161–168.
Alewu, B., & Nosiri, C. (Ed.). (2011). Pesticides in the Modern World: Effects of Pesticides Exposure. Mexico: InTechOpen.
Ali, M., CHENG, Z., Hayat, S., Ahmad, H., Ghani, M. I., & Tao, L. I. U. (2019). Foliar spraying of aqueous garlic bulb extract stimulates growth and antioxidant enzyme activity in eggplant (Solanum melongena L.). Journal of Integrative Agriculture, 18(5), 1001-1013. https://doi.org/10.1016/S2095-3119(18)62129-X
Aliu, S., Rusinovci, I., Fetahu, S., Salihu, S., & Zogaj, R. (2012). Nutritive and mineral composition in a collection of Cucurbita pepo L grown in Kosova. Food and Nutrition Sciences, 3(5), 634-638. https://doi.org/10.4236/fns.2012.35087
Amuji, C. F., Echezona, B. C., & Dialoke, S. A. (2012). Extraction fractions of ginger (Zingiber officinale Roscoe) and residue in the control of field and storage pests. Journal of Agricultural Technology, 8(6), 2023-2031.
Basra, S. M. A., & Lovatt, C. J. (2016). Exogenous applications of moringa leaf extract and cytokinins improve plant growth, yield, and fruit quality of cherry tomato. HortTechnology, 26(3), 327-337. https://doi.org/10.21273/HORTTECH.26.3.327
Baweja, P., Kumar, S., & Kumar, G. (Ed.).  (2020). Fertilizers and Pesticides: Their Impact on Soil Health and Environment (pp. 265-285). https://doi.org/10.1007/978-3-030-44364-1_15
Blanco-Díaz, M. T., Del Río-Celestino, M., Martínez-Valdivieso, D., & Font, R. (2014). Use of visible and near-infrared spectroscopy for predicting antioxidant compounds in summer squash (Cucurbita pepo ssp pepo). Food Chemistry, 164. https://doi.org/10.1016/j.foodchem.2014.05.019
Blunden, G., Currie, M., Mathe, J., Hohmann, J., & Critchley, A. T. (2009). Variations in betaine yields from marine algal species commonly used in the preparation of seaweed extracts used in agriculture. Phycology, 76, 14. https://doi.org/10.1177/1934578X1000500418
Buet, A., Costa, M. L., Martínez, D. E., & Guiamet, J. J. (2019). Chloroplast protein degradation in senescing leaves: Proteases and lytic compartments. Frontiers in Plant Science, 10, 451973. https://doi.org/10.3389/fpls.2019.00747
Carvalho, R. da S., Silva, M. A. da, Borges, M. T. M. R., & Forti, V. A. (2022). Plant extracts in agriculture and their applications in the treatment of seeds. Ciência Rural, 52(5), 1-18. https://doi.org/10.1590/0103-8478cr20210245
Chrysargyris, A., Xylia, P., Anastasiou, M., Pantelides, I., & Tzortzakis, N. (2018). Effects of Ascophyllum nodosum seaweed extracts on lettuce growth, physiology and fresh‐cut salad storage under potassium deficiency. Journal of the Science of Food and Agriculture, 98(15), 5861–5872. https://doi.org/10.1002/jsfa.9139
Chumark, P., Khunawat, P., Sanvarinda, Y., Phornchirasilp, S., Morales, N. P., Phivthong-Ngam, L., Ratanachamnong, P., Srisawat, S., & Klai-upsorn, S. P. (2008). The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. Journal of Ethnopharmacology, 116(3), 439–446. https://doi.org/10.1016/j.jep.2007.12.010
Craigie, J. S. (2011). Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology, 23, 371–393. https://doi.org/10.1007/s10811-010-9560-4
Elferink, M., & Schierhorn, F. (2016). Global demand for food is rising. Can we meet it. Harvard Business Review, 7(04), 2016.
Fan, D., Hodges, D. M., Critchley, A. T., & Prithiviraj, B. (2013). A commercial extract of brown macroalga (Ascophyllum nodosum) affects yield and the nutritional quality of spinach in vitro. Communications in Soil Science and Plant Analysis, 44(12), 1873–1884. https://doi.org/10.1080/00103624.2013.790404
FAO (Food and Agriculture Organization). (2022). Online Statistical Database: Food and Agriculture Organization of the World. FAOSTAT. [2023-07-15] http://www.fao.org/faostat/en/?#data/QC.
Food security information network (FSIN). (2020, September). 2020 Global report on food crises. Global Network against Food Crisis. https://www.fsinplatform.org/global-report-food-crises-2020
Godfray, H. C. J., Beddington, J. R., Crute, I. R., Haddad, L., Lawrence, D., Muir, J. F., Pretty, J., Robinson, S., Thomas, S. M., & Toulmin, C. (2010). Food security: the challenge of feeding 9 billion people. Science, 327(5967), 812–818. https://doi.org/10.1126/science.1185383
Goordeen, A., & Mohammed, M. (2021). Growth, development, maturation indices, proximate and mineral composition of moringa (Moringa oleifera). Journal of Horticulture and Postharvest Research, 4(4), 427-438. https://doi.org/10.22077/jhpr.2021.4296.1205
Hala, H., El-Noor, A., & Ewais, N. A. (2017). Effect of Moringa oleifera leaf extract (MLE) on pepper seed germination, seedlings improvement, growth, fruit yield and its quality. Middle East Journal of Agriculture Research, 6, 448–463.
Hayat, S., Ahmad, H., Ali, M., Ren, K., & Cheng, Z. (2018). Aqueous garlic extract stimulates growth and antioxidant enzymes activity of tomato (Solanum lycopersicum). Scientia Horticulturae, 240, 139–146. https://doi.org/10.1016/j.scienta.2018.06.011
Hidangmayum, A., & Sharma, R. (2017). Effect of different concentrations of commercial seaweed liquid extract of Ascophyllum nodosum as a plant bio stimulant on growth, yield and biochemical constituents of onion (Allium cepa L.). Journal of Pharmacognosy and Phytochemistry, 6(4), 658–663.
Jang, S. J., Park, H. H., & Kuk, Y. I. (2021). Application of various extracts enhances the growth and yield of cucumber (Cucumis sativus L.) without compromising the biochemical content. Agronomy, 11(3), 505. https://doi.org/10.3390/agronomy11030505
Kumari, R., Kaur, I., & Bhatnagar, A. K. (2011). Effect of aqueous extract of Sargassum johnstonii Setchell & amp; Gardner on growth, yield and quality of Lycopersicon esculentum Mill. Journal of Applied Phycology, 23(3), 623–633. https://doi.org/10.1007/s10811-011-9651-x
Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and carotenoids: Measurement and characterization by UV‐VIS spectroscopy. Current Protocols in Food Analytical Chemistry, 1(1), F4-3. https://doi.org/10.1002/0471142913.faf0403s01
Lust, T. A., & Paris, H. S. (2016). Italian horticultural and culinary records of summer squash (Cucurbita pepo, Cucurbitaceae) and emergence of the zucchini in 19th-century Milan. Annals of Botany, 118(1), 53–69. https://doi.org/10.1093/aob/mcw080
Manna, D., Sarkar, A., & Maity, T. K. (2012). Impact of biozyme on growth, yield and quality of chilli (Capsicum annuum L.). Journal of Crop and Weed, 8(1), 40-43.
Mbuyisa, S., Bertling, I., & Ngcobo, B. (2023). Impact of foliar-applied plant extracts on growth, physiological and yield attributes of the potato (Solanum tuberosum L.). Agronomy, 14(1), 38. https://doi.org/10.3390/agronomy14010038
Moore, K. K. (2004). Using seaweed compost to grow bedding plants. BioCycle, 45, 43-44.
Moyo, B., Masika, P. J., Arnold, H., & Voster, M. (2011). Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African Journal of Biotechnology, 10(60), 12925-12933. https://doi.org/10.5897/AJB10.1599
Ngcobo, B. L., & Bertling, I. (2021, November). Influence of foliar Moringa oleifera leaf extract (MLE) application on growth, fruit yield and nutritional quality of cherry tomato. II International Symposium on Moringa 1306. Pretoria, South Africa (pp. 249-254). Acta Horticulturae. https://doi.org/10.17660/ActaHortic.2021.1306.31
Ngcobo, B. L., & Mbuyisa, S. (2023, November). Recent developments in environmentally friendly techniques for extracting bioactive compounds from moringa plant parts. III International Symposium on Moringa 1394. Aracaju, Sergipe, Brazil (pp. 93-98). Acta Horticulturae. https://doi.org/10.17660/ActaHortic.2024.1394.12
Ngcobo, B., Bertling, I., & Mbuyisa, S. (2024). Evaluating the efficacy of Moringa oleifera leaf extracts prepared using different solvents on growth, yield and quality of tomatoes and peppers. Journal of Horticulture and Postharvest Research, 7(4), 389-406. https://doi.org/10.22077/jhpr.2024.7667.1383
Oloyede, F. M., Agbaje, G. O., Obuotor, E. M., & Obisesan, I. O. (2012). Nutritional and antioxidant profiles of pumpkin (Cucurbita pepo Linn.) immature and mature fruits as influenced by NPK fertilizer. Food Chemistry, 135(2), 460-463. https://doi.org/10.1016/j.foodchem.2012.04.124
Parajuli, R., Thoma, G., & Matlock, M. D. (2019). Environmental sustainability of fruit and vegetable production supply chains in the face of climate change: A review. Science of the Total Environment, 650, 2863-2879. https://doi.org/10.1016/j.scitotenv.2018.10.019
Paris, H. S. (2001). History of the cultivar-groups of Cucurbita pepo. Horticultural Reviews-Westport Then New York-, 25, 71-170. https://doi.org/10.1002/9780470650783.ch2
Rajendran, R., Jagmohan, S., Jayaraj, P., Ali, O., Ramsubhag, A., & Jayaraman, J. (2022). Effects of Ascophyllum nodosum extract on sweet pepper plants as an organic biostimulant in grow box home garden conditions. Journal of Applied Phycology, 34(1), 647-657. https://doi.org/10.1007/s10811-021-02611-z
Rayorath, P., Jithesh, M. N., Farid, A., Khan, W., Palanisamy, R., Hankins, S. D., Critchley, A. T., & Prithiviraj, B. (2008). Rapid bioassays to evaluate the plant growth promoting activity of Ascophyllum nodosum (L.) Le Jol. using a model plant, Arabidopsis thaliana (L.) Heynh. Journal of Applied Phycology, 20, 423-429. https://doi.org/10.1007/s10811-007-9280-6
Rioux, L.-E., Turgeon, S. L., & Beaulieu, M. (2007). Characterization of polysaccharides extracted from brown seaweeds. Carbohydrate Polymers, 69(3), 530-537. https://doi.org/10.1016/j.carbpol.2007.01.009
Saini, R. K., Sivanesan, I., & Keum, Y.-S. (2016). Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech, 6(2), 203. https://doi.org/10.1007/s13205-016-0526-3
Sharma, A., Kumar, V., Shahzad, B., Tanveer, M., Sidhu, G. P. S., Handa, N., Kohli, S. K., Yadav, P., Bali, A. S., Parihar, R. D., Dar, O. I., Singh, K., Jasrotia, S., Bakshi, P., Ramakrishnan, M., Kumar, S., Bhardwaj, R., & Thukral, A. K. (2019). Worldwide pesticide usage and its impacts on ecosystem. SN Applied Sciences, 1(11), 1446. https://doi.org/10.1007/s42452-019-1485-1
Ting-Ting, W., Zhi-Hui, C., Khan, M. A., Qing, M., & Ling, H. (2011). The inhibitive effects of garlic bulb crude extract on Fulvia fulva of tomato. Pakistan Journal of Botany, 43(5), 2575-2580.
Van Dijk, M., Morley, T., Rau, M. L., & Saghai, Y. (2021). A meta-analysis of projected global food demand and population at risk of hunger for the period 2010–2050. Nature Food, 2(7), 494-501. https://doi.org/10.1038/s43016-021-00322-9
Wang, T., Jónsdóttir, R., & Ólafsdóttir, G. (2009). Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds. Food Chemistry, 116(1), 240-248. https://doi.org/10.1016/j.foodchem.2009.02.041
Wheeler, T., & von Braun, J. (2013). Climate Change Impacts on Global Food Security. Science, 341(6145), 508-513. https://doi.org/10.1126/science.1239402
Yamaguchi, S. (2008). Gibberellin metabolism and its regulation. Annual Review of Plant Biology., 59, 225-251. https://doi.org/10.1146/annurev.arplant.59.032607.092804
Yaseen, A. A., & Takacs-Hajos, M. (2022). Evaluation of moringa (Moringa oleifera Lam.) leaf extract on bioactive compounds of lettuce (Lactuca sativa L.) grown under glasshouse environment. Journal of King Saud University - Science, 34(4), 101916. https://doi.org/10.1016/j.jksus.2022.101916
Zhang, D., Feng, Y., Li, N., & Sun, X. (2021). Fruit and vegetable consumptions in relation to frequent mental distress in breast cancer survivors. Supportive Care in Cancer, 29, 193-201. https://doi.org/10.1007/s00520-020-05451-8
Ziatabar Ahmadi, S. R., Seifi, E., Varasteh, F., & Akbarpour, V. (2024). Effect of biofertilizer inoculation on the growth and physiological traits of Red Angel and Wonderful pomegranate plantlets under salinity stress. Journal of Horticulture and Postharvest Research, 7(2), 171-182. https://doi.org/10.22077/jhpr.2024.7168.1356
Zulfiqar, F., Casadesús, A., Brockman, H., & Munné-Bosch, S. (2020). An overview of plant-based natural biostimulants for sustainable horticulture with a particular focus on moringa leaf extracts. Plant Science, 295, 110194. https://doi.org/10.1016/j.plantsci.2019.110194