Document Type : Original Article

Authors

1 Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Plant protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Purpose: Apples are susceptible to several diseases, which makes marketing and storage challenging. Therefore, it is critical to develop strategies that minimize weight loss while maintaining quality. Research method: Golden Delicious apples were coated with an edible mixture of Aloe vera gel (ALV) with or without Zataria multiflora essential oil (ZMO). Subsequently, the effects of these coatings on disease severity and incidence of Botrytis cinerea and Penicillium expansum, as well as storage quality features, were assessed. Inoculated apples with B. cinerea or P. expansum, the causative agents of apple postharvest gray and blue molds, were covered and stored at 25°C for 10 days and 2°C for 120 days. Findings: The antifungal ALV gel coatings significantly reduced the severity of gray and blue molds on inoculated apples, with the 150 μL L-1 of ZMO-based coating showing the highest effectiveness. The ALV+ZMO coatings had the best results, exhibiting a lower decay index, and increased firmness, total phenol content, and antioxidant activity in the coated apples. The highest ripening index was observed in the control samples, which ranged from the initial value of 37.0 to 54.7 by the end of the storage period. Meanwhile, ALV alone was the most effective at decreasing weight loss. Research limitations: Thyme essential oil (EO) has limitations when used directly, inculding its strong odor and flavor, low stability, low water solubility, and high volatility. Originality/Value: Overall, ALV and ZMO edible coatings appear to be a viable solution for suppressing fungal infections while maintaining apple quality under both situations.

Graphical Abstract

Enhancing decay resistance and maintaining quality of stored apples (Malus domestica ‘Golden Delicious’) through essential oil-enriched edible coatings

Keywords

Main Subjects

Avaei, A., Mohamadi Sani, A., & Mahmoodzadeh Vaziri, B. (2015). Chemical composition and antimicrobial effect of the essential oil of Zataria multiflora Boiss endemic in Khorasan-Iran. Asian Pacific Journal of Tropical Disease, 5(3), 181-185. https://doi.org/10.1016/S2222-1808(14)60649-6
Al-Hassanavi, H., Rostami Tobnag, G., & Shokri, R. (2023). Application of essential oils and optimizing storage conditions for control of postharvest diseases in apple. Advances in Horticultural Science, 37(2). https://doi.org/10.36253/ahsc-13919
Ali, A., Noh, N. M., & Mustafa, M. A. (2015). Antimicrobial activity of chitosan enriched with lemongrass oil against anthracnose of bell pepper. Food Packaging and Shelf Life, 3, 56-61. https://doi.org/10.1016/j.fpsl.2014.10.003
Dashipour, A., Razavilar, V., Hosseini, H., Shojaee-Aliabadi, S., German, J. B., Ghanati, K., Khakpour, M., & Khaksar, R. (2015). Antioxidant and antimicrobial carboxymethyl cellulose films containing Zataria multiflora essential oil. International journal of biological macromolecules, 72, 606-613. https://doi.org/10.1016/j.ijbiomac.2014.09.006
Dhital, R., Mora, N. B., Watson, D. G., Kohli, P., & Choudhary, R. (2018). Efficacy of limonene nano coatings on post-harvest shelf life of strawberries. Lwt, 97, 124-134. https://doi.org/10.1016/j.lwt.2018.06.038
Duan, X., Jing, G., Fan, F., & Tao, N. (2016). Control of postharvest green and blue molds of citrus fruit by application of sodium dehydroacetate. Postharvest Biology and Technology, 113, 17-19. https://doi.org/10.1016/j.postharvbio.2015.10.015
Esmaeili, Y., Paidari, S., Baghbaderani, S. A., Nateghi, L., Al-Hassan, A. A., & Ariffin, F. (2022). Essential oils as natural antimicrobial agents in postharvest treatments of fruits and vegetables: A review. Journal of Food Measurement and Characterization, 16, 507–522. https://doi.org/10.1007/s11694-021-01178-0
FAS, United States Department of Agriculture Foreign Agricultural Service https://fas.usda.gov/data/production/commodity/0574000 (accessed 2024).
Ghasemnezhad, M., Zareh, S., Rassa, M., & Sajedi, R. H. (2013). Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. Journal of the Science of Food and Agriculture, 93(2), 368-374. https://doi.org/10.1002/jsfa.5770
Gil, M. I., Tomás-Barberán, F. A., Hess-Pierce, B., Holcroft, D. M., & Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food chemistry, 48(10), 4581-4589. https://doi.org/10.1021/jf000404a
Glos, H., Bryk, H., Michalecka, M., & Puławska, J. (2022). The recent occurrence of biotic postharvest diseases of apples in Poland. Agronomy, 12(2), 399.     https://doi.org/10.3390/agronomy12020399
Habibi, A., Yazdani, N., Chatrabnous, N., Koushesh Saba, M., & Vahdati, K. (2022). Inhibition of browning via aqueous gel solution of Aloe vera: a new method for preserving fresh fruits as a case study on fresh kernels of Persian walnut. Journal of Food Science and Technology, 59, 2784–2793 https://doi.org/10.1007/s13197-021-05301-3
Hasan, M. U., Riaz, R., Malik, A. U., Khan, A. S., Anwar, R., Rehman, R. N. U., & Ali, S. (2021). Potential of Aloe vera gel coating for storage life extension and quality conservation of fruits and vegetables: An overview. Journal of Food Biochemistry, 45(4), e13640. https://doi.org/10.1111/jfbc.13640
Hashemi, M., Dastjerdi, A. M., Mirdehghan, S. H., Shakerardekani, A., & Golding, J. B. (2021). Incorporation of Zataria multiflora Boiss essential oil into gum Arabic edible coating to maintain the quality properties of fresh in-hull pistachio (Pistacia vera L.). Food Packaging and Shelf life, 30, 100724. https://doi.org/10.1016/j.fpsl.2021.100724
Hassan, H. S., EL-Hefny, M., Ghoneim, I. M., El-Lahot, M. S. A., Akrami, M., Al-Huqail, A. A., ALI H.M., & Abd-Elkader, D. Y. (2022). Assessing the use of aloe vera gel alone and in combination with lemongrass essential oil as a coating material for strawberry fruits: HPLC and EDX analyses. Coatings, 12(4), 489. https://doi.org/10.3390/coatings12040489
Hassanpour, H. (2015). Effect of Aloe vera gel coating on antioxidant capacity, antioxidant enzyme activities and decay in raspberry fruit. LWT-Food Science and Technology, 60(1), 495-501. https://doi.org/10.1016/j.lwt.2014.07.049
Hidayati, J. R., Yudiati, E., Pringgenies, D., Oktaviyanti, D. T., & Kusuma, A. P. (2020). Comparative study on antioxidant activities, total phenolic compound and pigment contents of tropical Spirulina platensis, Gracilaria arcuata and Ulva lactuca extracted in different solvents polarity. In E3S Web of Conferences (Vol. 147, p. 03012). EDP Sciences.
Jahanshahi, B., Jafari, A., & Gholamnezhad, J. (2023). Effect of edible tragacanth coating on fruit quality of tomato cv. Falkato. Journal of Horticulture and Postharvest Research, 6(1), 43-54. https://doi.org/10.22077/jhpr.2022.5478.1282
Jiwanit, P., Pitakpornpreecha, T., Pisuchpen, S., & Leelasuphakul, W. (2018). The use of Aloe vera gel coating supplemented with Pichia guilliermondii BCC5389 for enhancement of defense-related gene expression and secondary metabolism in mandarins to prevent postharvest losses from green mold rot. Biological control, 117, 43-51. https://doi.org/10.1016/j.biocontrol.2017.08.023
Jo, W. S., Song, H. Y., Song, N. B., Lee, J. H., Min, S. C., & Song, K. B. (2014). Quality and microbial safety of ‘Fuji’apples coated with carnauba-shellac wax containing lemongrass oil. LWT-Food Science and Technology, 55(2), 490-497. https://doi.org/10.1016/j.lwt.2013.10.034
Kharchoufi, S., Parafati, L., Licciardello, F., Muratore, G., Hamdi, M., Cirvilleri, G., & Restuccia, C. (2018). Edible coatings incorporating pomegranate peel extract and biocontrol yeast to reduce Penicillium digitatum postharvest decay of oranges. Food Microbiology, 74, 107-112. https://doi.org/10.1016/j.fm.2018.03.011
Khatri, D., Panigrahi, J., Prajapati, A., & Bariya, H. (2020). Attributes of Aloe vera gel and chitosan treatments on the quality and biochemical traits of post-harvest tomatoes. Scientia Horticulturae, 259, 108837. https://doi.org/10.1016/j.scienta.2019.108837
Kowalczyk, D., Kordowska-Wiater, M., Zięba, E., & Baraniak, B. (2017). Effect of carboxymethylcellulose/candelilla wax coating containing potassium sorbate on microbiological and physicochemical attributes of pears. Scientia Horticulturae, 218, 326-333. https://doi.org/10.1016/j.scienta.2017.02.040
Lahooji, A., Mirabolfathy, M., & Karami-Osboo, R. (2010). Effect of Zataria multiflora and Satureja hortensis essential oils, thymol and carvacrol on growth of Fusarium gramineum isolates and deoxynivalenol production. Iranian Journal of Plant Pathology, 46, 37-50.
Laribi, A. I., Palou, L., Taberner, V., & Pérez-Gago, M. B. (2012). Modified atmosphere packaging to extend cold storage of pomegranate cv.'Mollar de Elche'. In Proceedings of the International Conference of Agricultural Engineering—CIGR-AgEng.
Li, J., Lei, H., Song, H., Lai, T., Xu, X., & Shi, X. (2017). 1-methylcyclopropene (1-MCP) suppressed postharvest blue mold of apple fruit by inhibiting the growth of Penicillium expansum. Postharvest Biology and Technology, 125, 59-64. https://doi.org/10.1016/j.postharvbio.2016.11.005
Luciano‐Rosario, D., Keller, N. P., & Jurick, W. M. (2020). Penicillium expansum: Biology, omics, and management tools for a global postharvest pathogen causing blue mould of pome fruit. Molecular Plant Pathology, 21(11), 1391-1404. https://doi.org/10.1111/mpp.12990
Mannucci, A., Serra, A., Remorini, D., Castagna, A., Mele, M., Scartazza, A., & Ranieri, A. (2017). Aroma profile of Fuji apples treated with gelatin edible coating during their storage. LWT-Food Science and Technology, 85, 28-36. https://doi.org/10.1016/j.lwt.2017.06.061
Maqbool, M., Ali, A., Alderson, P. G., Mohamed, M. T. M., Siddiqui, Y., & Zahid, N. (2011). Postharvest application of gum arabic and essential oils for controlling anthracnose and quality of banana and papaya during cold storage. Postharvest Biology and Technology, 62(1), 71-76. https://doi.org/10.1016/j.postharvbio.2011.04.002
Martínez-Romero, D., Zapata, P. J., Guillén, F., Paladines, D., Castillo, S., Valero, D., & Serrano, M. (2017). The addition of rosehip oil to Aloe gels improves their properties as postharvest coatings for maintaining quality in plum. Food Chemistry, 217, 585-592. https://doi.org/10.1016/j.foodchem.2016.09.035
Mendy, T. K., Misran, A., Mahmud, T. M. M., & Ismail, S. I. (2019). Application of Aloe vera coating delays ripening and extend the shelf life of papaya fruit. Scientia Horticulturae, 246, 769-776. https://doi.org/10.1016/j.scienta.2018.11.054
Mohammadi, A., Hashemi, M., & Hosseini, S. M. (2016). Integration between chitosan and Zataria multiflora or Cinnamomum zeylanicum essential oil for controlling Phytophthora drechsleri, the causal agent of cucumber fruit rot. LWT-Food Science and Technology, 65, 349-356. https://doi.org/10.1016/j.lwt.2015.08.015
Moradinezhad, F., & Ranjbar, A. (2023). Advances in postharvest diseases management of fruits and vegetables: A review. Horticulturae, 9(10), 1099. https://doi.org/10.3390/horticulturae9101099
Mostafidi, M., Sanjabi, M. R., Shirkhan, F., & Zahedi, M. T. (2020). A review of recent trends in the development of the microbial safety of fruits and vegetables. Trends in Food Science & Technology, 103, 321-332. https://doi.org/10.1016/j.tifs.2020.07.009
Motamedi, E., Nasiri, J., Malidarreh, T. R., Kalantari, S., Naghavi, M. R., & Safari, M. (2018). Performance of carnauba wax-nanoclay emulsion coatings on postharvest quality of ‘Valencia’ orange fruit. Scientia Horticulturae, 240, 170-178. https://doi.org/10.1016/j.scienta.2018.06.002
Nair, M. S., Saxena, A., & Kaur, C. (2018). Effect of chitosan and alginate based coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava L.). Food chemistry, 240, 245-252. https://doi.org/10.1016/j.foodchem.2017.07.122
Nasiri, M., Barzegar, M., Sahari, M. A., & Niakousari, M. (2017). Tragacanth gum containing Zataria multiflora Boiss. essential oil as a natural preservative for storage of button mushrooms (Agaricus bisporus). Food Hydrocolloids, 72, 202-209. https://doi.org/10.1016/j.foodhyd.2017.05.045
Navarro, D., Díaz-Mula, H. M., Guillén, F., Zapata, P. J., Castillo, S., Serrano, M., Valero, D., & Martínez-Romero, D. (2011). Reduction of nectarine decay caused by Rhizopus stolonifer, Botrytis cinerea and Penicillium digitatum with Aloe vera gel alone or with the addition of thymol. International Journal of Food Microbiology, 151(2), 241-246. https://doi.org/10.1016/j.ijfoodmicro.2011.09.009
Oliveira, D. G. P., Pauli, G., Mascarin, G. M., & Delalibera, I. (2015). A protocol for determination of conidial viability of the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae from commercial products. Journal of Microbiological Methods, 119, 44-52. https://doi.org/10.1016/j.mimet.2015.09.021
Panchal, N., Das, K., Prabhakar, P. K., & Ghanghas, N. (2022). Edible films and coatings for fruits and vegetables: Composition, functions, and regulatory aspects. Edible Food Packaging: Applications, Innovations and Sustainability, 191-216. https://doi.org/10.1007/978-981-16-2383-7_10
Pandey, A., & Singh, S. (2016). Aloe Vera: A Systematic Review of its Industrial and Ethno-Medicinal Efficacy. International Journal of Pharmaceutical Research & Allied Sciences, 5(1), 1-21.
Rahimi Kakolaki, M., Omidi, A., Rasooli, A., & Shekarforoush, S. S. (2024). In vitro antifungal activity   of barberry fruit extract (Berberis spp.) against Fusarium spp.. Journal of Horticulture and Postharvest Research, 7(Special Issue - Postharvest Technologies), 47-60. http://dx.doi.org/10.22077/jhpr.2023.6783.1333
Ramezanian, A., Azadi, M., Mostowfizadeh-Ghalamfarsa, R., & Saharkhiz, M. J. (2016). Effect of Zataria multiflora Boiss and Thymus vulgaris L. essential oils on black rot of ‘Washington Navel’ orange fruit. Postharvest Biology and Technology, 112, 152-158. https://doi.org/10.1016/j.postharvbio.2015.10.011
Rehman, M. A., Asi, M. R., Hameed, A., & Bourquin, L. D. (2020). Effect of Postharvest application of Aloe vera gel on shelf life, activities of anti-oxidative enzymes, and quality of ‘Gola’ guava fruit. Foods, 9(10), 1361. https://doi.org/10.3390/foods9101361
Shehata, S. A., Abdeldaym, E. A., Ali, M. R., Mohamed, R. M., Bob, R. I., & Abdelgawad, K. F. (2020). Effect of some citrus essential oils on post-harvest shelf life and physicochemical quality of strawberries during cold storage. Agronomy, 10(10), 1466. https://doi.org/10.3390/agronomy10101466
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.
Synowiec, A., Gniewosz, M., Kraśniewska, K., Przybył, J. L., Bączek, K., & Węglarz, Z. (2014). Antimicrobial and antioxidant properties of pullulan film containing sweet basil extract and an evaluation of coating effectiveness in the prolongation of the shelf life of apples stored in refrigeration conditions. Innovative Food Science & Emerging Technologies, 23, 171-181. https://doi.org/10.1016/j.ifset.2014.03.006
Supa, S., Howlader, P., Ali, M., Rupa, R., & Bose, S. (2024). Edible coatings maintained postharvest quality and increased shelf life of guava fruits. Journal of Horticulture and Postharvest Research, 7(Special Issue - Postharvest Technologies), 15-34. https://doi.org/10.22077/jhpr.2023.6531.1324
Thakur, R., Pristijono, P., Golding, J. B., Stathopoulos, C. E., Scarlett, C. J., Bowyer, M., & Vuong, Q. V. (2018). Development and application of rice starch based edible coating to improve the postharvest storage potential and quality of plum fruit (Prunus salicina). Scientia Horticulturae, 237, 59-66. https://doi.org/10.1016/j.scienta.2018.04.005
Valero, D., Díaz-Mula, H. M., Zapata, P. J., Guillén, F., Martínez-Romero, D., Castillo, S., & Serrano, M. (2013). Effects of alginate edible coating on preserving fruit quality in four plum cultivars during postharvest storage. Postharvest Biology and Technology, 77, 1-6. https://doi.org/10.1016/j.postharvbio.2012.10.011
Zapata, P. J., Navarro, D., Guillén, F., Castillo, S., Martínez-Romero, D., Valero, D., & Serrano, M. (2013). Characterisation of gels from different Aloe spp. as antifungal treatment: Potential crops for industrial applications. Industrial Crops and Products, 42, 223-230. https://doi.org/10.1016/j.indcrop.2012.06.002