Document Type : Original Article

Authors

Department of Horticulture, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Purpose: The ‘Braeburn’ apple, cultivated in Mashhad, Iran, has poor red coloration that affecting its marketability. This research studied the effects of mono-potassium phosphate (MKP) and calcium prohexadione (ProCa) on red pigment development and biochemical traits, as well as interactions with two rootstocks used for grafting. Research method: The experimental treatments included four concentrations of MKP (0, 0.1, 1.0, and 2.0 g/L) sprayed in three periods: 20 days after petal fall, 45 and 30 days before commercial fruit harvest. Furthermore, ProCa was also applied in four concentrations (0, 125, 250, and 500 mg/L), one month before harvest (three times with 10-day intervals). Findings: The results showed that M.9 rootstock led to larger fruit diameters compared to MM.111, but overall physical traits remained unchanged. Chemical applications significantly affected fruit diameter and firmness, with ProCa treatments (250 and 500 mg/L) yielding the highest firmness levels. Rootstock type influenced total acidity (TA), total soluble solids (TSS), flavor index, and vitamin C content. M.9 rootstock combined with MKP spray resulted in the best TA, TSS, and flavor index. However, higher ProCa concentrations negatively impacted color development and anthocyanin levels. Thus, cultivating red apple cultivars in low-altitude regions like Mashhad is not recommended due to environmental factors affecting pigmentation. Research limitations: There was no apple orchard of the same variety located in a higher altitude within the same region for a comparative analysis. Originality/Value: The article clearly emphasizes that the orchard establishment of the ‘Braeburn’ apple is not technically authorized for low altitude places (lower than 1000 m).

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Main Subjects

Abe, K. J., Soejima, Iwanami H, Kotoda N, Moriya S, Bessho H, Komori S, Ito Y, Takahashi S, Okada K, Kato H, Haji T, Ishiguro M, Masuda T & Tsuchiya S. (2017). New apple cultivar ‘Rose Pearl’. Bull. NARO Inst. Fruit Tree & Tea Science, 1, 9–18 (In Japanese with English abstract). https://www.cabidigitallibrary.org
Amarante, C. V. T. D., Silveira, J. P. G., Freitas, S. T. D., Steffens, C. A., & Mitcham, E. J. (2021). Fruit quality of ‘Braeburn’apple trees sprayed at post-bloom and preharvest with prohexadione-calcium and GA4+7. Revista Brasileira de Fruticultura, 43(1), 653. https://doi.org/10.1590/0100-29452021653
Ashwell, G. (1957). Colorimetric analysis of sugars. Methods in Enzynzology, (3), 73-105.
Barnes, J. D., Balaguer, L., Manrique, E., Elvira, S., & Davison, A. W. (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environmental and Experimental Botany, 32(2), 85-100. https://doi.org/10.1016/0098-8472(92)90034-Y
‏Bizjak, J., Weber, N., Mikulic-Petkovsek, M., Slatnar, A., Stampar, F., Alam, Z., & Veberic, R. (2013). Influence of Phostrade Ca on color development and anthocyanin content of ‘Braeburn’apple (Malus domestica Borkh.). HortScience, 48(2), 193-199. https://doi.org/10.21273/HORTSCI.48.2.193
Blanke, M. M. (2008). Alternatives to reflective mulch cloth (Extenday™) for apple under hail net?. Scientia Horticulturae, 116(2), 223-226. https://doi.org/10.1016/j.scienta.2007.12.004
Brighenti, A. F., Würz, D. A., Pasa, M. D. S., & Rufato, L. (2017). Plant growth regulators to enhance fruit color of 'Gala' apples. Pesquisa Agropecuária Brasileira52(11), 1118-1122. https://doi.org/10.1590/S0100-204X2017001100018
Brunetto, G., MELO, G. W. B. D., Toselli, M., Quartieri, M., & Tagliavini, M. (2015). The role of mineral nutrition on yields and fruit quality in grapevine, pear and apple. Revista Brasileira de Fruticultura37, 1089-1104. https://doi.org/10.1590/0100-2945-103/15
Castle, W. S. (1995). Rootstock as a fruit quality factor in citrus and deciduous tree crops. New Zealand Journal of Crop and Horticultural Science23(4), 383-394. https://doi.org/10.1080/01140671.1995.9513914
Chen, W., Zhang, M., Zhang, G., Li, P., & Ma, F. (2019). Differential regulation of anthocyanin synthesis in apple peel under different sunlight intensities. International Journal of Molecular Sciences, 20(23), 6060. https://doi.org/10.3390/ijms20236060
Cline, J. A., Embree, C. G., Hebb, J., & Nichols, D. S. (2008). Performance of prohexadione-calcium on shoot growth and fruit quality of apple: Effect of spray surfactants. Canadian Journal of Plant Science88(1), 165-174. https://doi.org/10.4141/CJPS07087
Dar, J. A., Wani, A. A., Ahmed, M., Nazir, R., Zargar, S. M., & Javaid, K. (2019). Peel colour in apple (Malus domestica Borkh.): An economic quality parameter in fruit market. Scientia Horticulturae244, 50-60. https://doi.org/10.1016/j.scienta.2018.09.029
De Freitas, S. T., do Amarante, C. V., Labavitch, J. M., & Mitcham, E. J. (2010). Cellular approach to understand bitter pit development in apple fruit. Postharvest Biology and Technology57(1), 6-13. https://doi.org/10.1016/j.postharvbio.2010.02.006
Dzida, K., Michałojć, Z., Jarosz, Z., Pitura, K., & Skubij, N. (2018). Effect of potassium fertilization on yield, growth and chemical composition of basil herb. Acta Scientiarum Polonorum Hortorum Cultus, 17(6), 135-145.
Elzebroek, A. T. G. (2008). Guide to cultivated plants. CABI. UK, 540 p.
Fallahi, E., Arzani, K., & Fallahi, B. (2013). Long-term leaf mineral nutrition in ‘Pacific Gala’apple (Malus domestica Borkh.) as affected by rootstock type and irrigation system during six stages of tree development. The Journal of Horticultural Science and Biotechnology88(6), 685-692. https://doi.org/10.1080/14620316.2013.11513025
Fallahi, E., Neilsen, D., Neilsen, G. H., Fallahi, B., & Shafii, B. (2010). Efficient irrigation for optimum fruit quality and yield in apples. HortScience45(11), 1616-1625. https://doi.org/10.21273/HORTSCI.45.11.1616
FAOSTAT, F. (2022). Food and agriculture organization of the United Nations. Statistical database. Available at: http://faostat. Fao.Org/336.default.Asp.
Gouws, A., & Steyn, W. J. (2014). The effect of temperature, region and season on red colour development in apple peel under constant irradiance. Scientia Horticulturae173, 79-85. https://doi.org/10.1016/j.scienta.2014.04.040
Habibzadeh L. (2022). Effect of Rootstock and application of some chemicals on physico-chemical parameters and red color development of Braeburn apple fruits in Khorasan-e- Razavi. MSc Thesis, Horticulture Department, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Habibzadeh L., Alizadeh M, Sharifani M. (2022). Effective factors in the development of red color of apple fruit (Malus domestica). 4th International Congress of Agricultural Engineering, Natural Resources, Environment, 16th December, 2022, Tehran, Iran.
Harris, S. A., Robinson, J. P., & Juniper, B. E. (2002). Genetic clues to the origin of the apple. TRENDS in Genetics, 18(8), 426-430. https://doi.org/10.1016/s0168-9525(02)02689-6.
Honda, C., & Moriya, S. (2018). Anthocyanin biosynthesis in apple fruit. The Horticulture Journal87(3), 305-314. https://doi.org/10.2503/hortj.OKD-R01
Iglesias, I., Echeverría, G., & Soria, Y. (2008). Differences in fruit colour development, anthocyanin content, fruit quality and consumer acceptability of eight ‘Gala’ apple strains. Scientia Horticulturae119(1), 32-40. https://doi.org/10.1016/j.scienta.2008.07.004
Jahani, M., Sayyari Zohan, M., Moradinezhad, F., Mirzaee, M. R., & Khayyat, M. (2024). Effectiveness of potassium spraying on mitigation of aril paleness disorder in different pomegranate cultivars. Journal of Plant Nutrition, 47(18), 3024-3034. https://doi.org/10.1080/01904167.2024.2376232
Kamiab, F., Tavassolian, I., & Hosseinifarahi, M. (2023). Changes in the quantitative and qualitative characteristics of seedless barberry (Berberis Vulgaris L.) fruit as influenced by fruit thinning. Journal of Horticulture and Postharvest Research, 6(1), 77-92. https://doi.org/10.22077/jhpr.2022.5416.1279
Karagiannis, E., Michailidis, M., Tanou, G., Scossa, F., Sarrou, E., Stamatakis, G., & Molassiotis, A. (2020). Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening. Horticulture Research, 1(7), 120. https://doi.org/10.1038/s41438-020-00340-x
Kashyap, G., & Gautam, M. D. (2012). Analysis of vitamin C in commercial and naturals substances by iodometric titration found in nimar and malwa regeion. Journal of Scientific Research in Pharmacy1(2), 77-78.
Kim, S. H., Lee, J. R., Hong, S. T., Yoo, Y. K., An, G., & Kim, S. R. (2003). Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin. Plant Science165(2), 403-413. https://doi.org/10.1016/S0168-9452(03)00201-2
Kumar, A., Bhuj, B. D., & Dhar, S. (2024). New approaches of rootstocks in fruit production: a review. Open Access Journal of Botanical Insights, 2(1), 000109.
Kuzin, A. I., Kashirskaya, N. Y., Kochkina, A. M., & Kushner, A. V. (2020). Correction of potassium fertigation rate of apple tree (Malus domestica Borkh.) in central Russia during the growing season. Plants9(10), 1366. https://doi.org/10.3390/plants9101366
Li, Z., Gemma, H., & Iwahori, S. (2002). Stimulation of ‘Fuji’ apple skin color by ethephon and phosphorus–calcium mixed compounds in relation to flavonoid synthesis. Scientia Horticulturae94(1-2), 193-199. https://doi.org/10.1016/S0304-4238(01)00363-6
Lueangprasert, K., Uthaibutra, J., Saengnil, K., & Arakawa, O. (2010). The effects of sugar application on the concentrations of anthocyanin and flavonol of ‘Mahajanaka’ mango (Mangifera indica Linn. cv. Mahajanaka) fruit. Chiang Mai Journal of Science37, 355-362.
Matsuoka, K. (2019). Anthocyanins in apple fruit and their regulation for health benefits. Flavonoid–a Coloring Model for Cheering Up Life. IntechOpen; Available from: http://dx.doi.org/10.5772/intechopen.85257.
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428.
Moradinezhad, F., Dorostkar, M., Niazmand, R., & Doraki, G. (2024). A comprehensive study of qualitative and biochemical characteristics of dried seedless barberry fruits from different regions of South Khorasan Province, Iran. Journal of Horticulture and Postharvest Research, 7(4), 345-360. https://doi.org/10.22077/jhpr.2024.7912.1399
Musacchi, S., & Serra, S. (2018). Apple fruit quality: Overview on pre-harvest factors. Scientia Horticulturae234, 409-430. https://doi.org/10.1016/j.scienta.2017.12.057
Nava, G., Dechen, A. R., & Nachtigall, G. R. (2007). Nitrogen and potassium fertilization affect apple fruit quality in southern Brazil. Communications in Soil Science and Plant Analysis, 39(1-2), 96-107. https://doi.org/10.1080/00103620701759038
Neilsen, G. H., Neilsen, D., Herbert, L. C., & Hogue, E. J. (2004). Response of apple to fertigation of N and K under conditions susceptible to the development of K deficiency. Journal of the American Society for Horticultural Sciences, 129(1), 26-31.
Njira, K. O., & Nabwami, J. (2015). A review of effects of nutrient elements on crop quality. African Journal of Food, Agriculture, Nutrition and Development, 15(1), 9777-9793. https://doi.org/10.18697/ajfand.68.13750
Omokolo Ndoumou, D., Tsala Ndzomo, G., & Djocgoue, P. F. (1996). Changes in carbohydrate, amino acid and phenol contents in cocoa pods from three clones after infection with phytophthora megakarya Bra. and Grif. Annals of Botany77(2), 153-158.
Plunkett, B. J., Henry-Kirk, R., Friend, A., Diack, R., Helbig, S., Mouhu, K., ... & Allan, A. C. (2019). Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes. Scientific Reports9(1), 17762. https://doi.org/10.1038/s41598-019-54166-2.
Robinson, T., & Lopez, S. (2010, August). Crop load affects'Honeycrisp'fruit quality more than Nitrogen, Potassium, or irrigaion. In XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on the 940 (pp. 529-537). https://doi.org/10.17660/ActaHortic.2012.940.76
Saure, M. C. (1990). External control of anthocyanin formation in apple. Scientia Horticulturae, 42(3), 181-218. https://doi.org/10.1016/0304-4238(90)90082-P
Shahkoomahally, S., Chaparro, J. X., Beckman, T. G., & Sarkhosh, A. (2020). Influence of rootstocks on leaf mineral content in the subtropical peach cv. UFSun. HortScience55(4), 496-502. https://doi.org/10.21273/HORTSCI14626-19
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture16(3), 144-158.
Solhjoo, S., Gharaghani, A., & Fallahi, E. (2017). Calcium and potassium foliar sprays affect fruit skin color, quality attributes, and mineral nutrient concentrations of ‘Red Delicious’ apples. International Journal of Fruit Science17(4), 358-373. https://doi.org/10.1080/15538362.2017.1318734
Tijskens, L. M. M., Unuk, T., Tojnko, S., Hribar, J., & Simcic, M. (2011). Colour development in the apple orchard. Journal of Fruit and Ornamental Plant Research19(1), 113-121.
Wagner, G. J. (1979). Content and vacuole/extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts. Plant Physiology, 64(1), 88-93. https://doi.org/10.1104/pp.64.1.88
Whale, S. K., Singh, Z., Behboudian, M. H., Janes, J., & Dhaliwal, S. S. (2008). Fruit quality in ‘Cripp's Pink’apple, especially colour, as affected by preharvest sprays of aminoethoxyvinylglycine and ethephon. Scientia Horticulturae115(4), 342-351. https://doi.org/10.1016/j.scienta.2007.10.015
Xu, X., Du, X., Wang, F., Sha, J., Chen, Q., Tian, G., ... & Jiang, Y. (2020). Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Frontiers in Plant Science11, 904. https://doi.org/10.3389/fpls.2020.00904
Yaldız, G., Özen, F., Çamlıca, M., & Sönmez, F. (2018). Alleviation of salt stress by increasing potassium sulphate doses in four medicinal and aromatic plants. Acta Agriculturae Scandinavica, Section B—Soil & Plant Science68(5), 437-447. https://doi.org/10.1080/09064710.2017.1420214