Influence of pre-harvest methyl jasmonate application on the fruit quality of strawberry cv. Paros at harvest and during cold storage

Meighani, Hossein; Salehi Sarbijan, Mohammad

doi:10.22077/jhpr.2025.9281.1506

Articles in Press

Document Type : Original Article

Authors

¹ Department of Horticultural Science, Faculty of Agriculture, University of Birjand, Birjand, Iran

² Department of Horticultural Science, Faculty of Agriculture, University of Jirot, Jiroft, Iran

https://doi.org/10.22077/jhpr.2025.9281.1506

Abstract

Purpose: Strawberry fruits are perishable with a short shelf life. Methyl jasmonate (MJ) is a well-known signaling molecule involved in the regulation of many processes in plants. Previous studies have addressed the effectiveness of pre- and postharvest MJ treatments on the quality of fruits. Therefore, in this study, the effect of pre-harvest MJ on the quality and physio-chemical traits of strawberry at harvest and during cold storage were investigated. Research Method: The effect of pre-harvest foliar spraying of MJ (0, 50, 100, and 200 μM) at three times (full flowering, production of green fruits, and the beginning of the pink stage) on quality and bioactive compounds of strawberry fruit (cv. Paros) stored at 4 °C for 12 d was studied. Findings: Our results showed that fruit obtained from MJ-treated plants had significantly higher firmness and APX enzyme activity than fruit from control plants. However, no significant differences in other traits were observed between control and MJ-treated fruits at this stage. During cold storage, MJ treatment significantly reduced firmness loss, physiological loss in weight, and ascorbic acid content compared to control fruits. In cold storage, MJ treatment reduced firmness loss and weight loss, while maintaining higher levels of titratable acidity, antioxidant compounds (ascorbic acid, anthocyanins, and phenolics), and total antioxidant activity. Furthermore, MJ treatment resulted in increased catalase and ascorbate peroxidase enzyme activity, as well as lowered guaiacol-peroxidase, total soluble solids/ titratable acidity ratio. Total soluble solids were not affected by MJ treatment at harvest and during cold storage. Research limitations: No limitations were found. Originality/Value: Pre-harvest application of MJ, especially 200 μM, can increase the shelf life of strawberry fruits by increasing or maintaining higher levels of bioactive compounds and antioxidant enzyme activity.

Keywords

Main Subjects

Postharvest Biology and Technology

References

Adl, E., Jahani, M. & Aminifard, M. H. (2024). Investigating the antifungal effects of essential oils on Aspergillus sp. in strawberry (Fragaria ananassa Duchesne) fruit. Journal of Horticulture and Postharvest Research, 7(2), 141-154. https://doi.org/10.22077/jhpr.2024.7065.1349

Ağlar, E., & Öztürk, B. (2018). Effects of pre-harvest methyl jasmonate treatments on fruit quality of fuji apples during cold storage. International Journal of Agriculture and Wildlife Science, 4(1), 13-19. https://doi.org/10.24180/ijaws.366304.

Ali, A., Kumar, A., Ganai, N. A., Dar, K. R., & Wani, A. H. (2021). Salicylic acid alleviates postharvest fruit decay of strawberry (Fragaria x ananassa Duch.) a review. International Journal of Plant and Soil Science, 33(20), 20-27. https://doi.org/10.9734/IJPSS/2021/v33i2030625.

AOAC (2000). Vitamin C. Ascorbic acid in vitamin preparations and juices. In: Helrich K (ed) Official methods of analysis, 15th edn. AOAC, Arlington, pp 1058–1059.

Asgari, F., Kalateh Jari, S., Motesharezadeh, B., Ghanbari Jahromi, M., & Weisany, W. (2024). Application of benzylaminopurine with methyl jasmonate and epibrassinolide improved growth and physio-biochemical attributes of strawberry (Fragaria × ananassa cv. ‘Albion’). Applied Fruit Science, 66, 453–463. https://doi.org/10.1007/s10341-023-00996-4.

Asghari, M., & Hasanlooe, A.R. (2016). Methyl jasmonate effectively enhanced some defense enzymes activity and Total Antioxidant content in harvested “Sabrosa” strawberry fruit. Food Science and Nutrition, 4, 377–383. https://doi.org/10.1002/fsn3.300.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lwt Food Science and Technology, 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5.

Cao, S., Zheng, Y., Yang, Z., Wang, K., & Rui, H. (2009). Effect of methyl jasmonate on quality and antioxidant activity of postharvest loquat fruit. Journal of the Science of Food and Agriculture, 89(12), 2064–2070. https://doi.org/10.1002/jsfa.3691.

Chanjirakul, K., Wang, S. Y., Wang, C. Y., & Siriphanich, J. (2006). Effect of natural volatile compounds on antioxidant capacity and antioxidant enzymes in raspberries. Postharvest Biology and. Technology, 40, 106–115. https://doi.org/10.1016/j.postharvbio.2006.01.004.

Concha, C. M., Figueroa, N. E., Poblete, L. A., Oñate, F. A., Schwab, W., & Figueroa, C. R. (2013). Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit. Plant Physiology and Biochemistry, 70, 433-444. https://doi.org/10.1016/j.plaphy.2013.06.008.

Darwish, O. S., Ali, M. R., Khojah, E., Samra, B. N., Ramadan, K. M. A., & El-Mogy, M. M. (2021). Pre-harvest application of salicylic acid, abscisic acid, and methyl jasmonate conserve bioactive compounds of strawberry fruits during refrigerated storage. Horticulturae, 7(12), 568. https://doi.org/10.3390/horticulturae7120568.

García-Pastor, M. E., Serrano, M., Guill´ en, F., Gim´enez, M. J., Martínez-Romero, D., Valero, D., & Zapata, P. J. (2020). Preharvest application of methyl jasmonate increases crop yield, fruit quality and bioactive compounds in pomegranate ‘Mollar de Elche’ at harvest and during postharvest storage. Journal of the Science of Food and Agriculture, 100(1), 145–153. https://doi.org/10.1002/jsfa.10007.

Han, Y., Chen, C., Yan, Z., Li, J., & Wang, Y. (2019). The methyl jasmonate accelerates the strawberry fruits ripening process. Scientia Horticulturae, 249, 250-256. https://doi.org/10.1016/j.scienta.2019.01.061.

Hasan, M. U., Singh, Z., Shah, H. M. S., Kaur, J., Woodward, A., Afrifa-Yamoah, E., & Vithana, M. D. K. (2025). Preharvest methyl jasmonate application regulates ripening, colour development and improves phytochemical quality of fruits: A review. Scientia Horticulturae, 339, 113909. https://doi.org/10.1016/j.scienta.2024.113909.

Ilea, M. I. M., Díaz-Mula, H. M., García-Molina, A., Ruiz-Aracil, M. C., Fernández-Picazo, C., & Guillén, F. (2025). Comparative effect of GABA and 1-MCP in maintaining strawberry fruit quality during cold storage. Horticulturae, 11(4), 370. https://doi.org/10.3390/horticulturae11040370.

Li, J., Azam, M., Noreen, A., Umer, M. A., Ilahy, R., Akram, M. T., Qadri, R., Khan, M. A., Rehman, S. u., Hussain, I., Lin, Q., & Liu, H. (2023). Application of methyl jasmonate to papaya fruit stored at lower temperature attenuates chilling injury and enhances the antioxidant system to maintain quality. Foods, 12(14), 2743. https://doi.org/10.3390/foods12142743.

Li, X., Li, M., Wang, L., Wang, J., Jin, P., & Zheng, Y. (2018). Methyl jasmonate primes defense responses against wounding stress and enhances phenolic accumulation in fresh-cut pitaya fruit. Postharvest Biology and Technology, 145, 101-107. https://doi.org/10.1016/j.postharvbio.2018.07.001.

Liao, L., Li, S., Li, Y., Huang, Z., Li, J., Xiong, B., Zhang, M., Sun, G., & Wang, Z. (2022). Pre- or postharvest treatment with MeJA improves postharvest storage of lemon fruit by stimulating the antioxidant system and alleviating chilling injury. Plants, 11, 2840. https://doi.org/10.3390/plants11212840.

Meighani, H., & Roozkhosh, M. (2024). Preserving the postharvest quality of strawberry cv. ‘sabrina’ by carboxymethyl cellulose and putrescine. Applied Fruit Science, 66, 51-59. https://doi.org/10.1007/s10341-023-01017-0.

Moya-León, M. A., Mattus-Araya, E., & Herrera, R. (2019). Molecular events occurring during softening of strawberry fruit. Frontiers in Plant Science, 10, 615. https://doi.org/10.3389/fpls.2019.00615.

Nguyen, D. H. H., & Nguyen, H. V. H. (2021). Effects of storage temperature on postharvest physico-chemical attributes of nano-chitosan coated strawberry (Fragaria × ananassa Duch.). Journal of Horticulture and Postharvest Research, 4(1), 101-114. https://doi.org/10.22077/jhpr.2020.3317.1139.

Saavedra, G. M., Figueroa, N. E., Poblete, L. A., Cherian, S., & Figueroa, C. R. (2016). Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of Fragaria chiloensis fruit. Food Chemistry, 190, 448 -453. https://doi.org/10.1016/j.foodchem.2015.05.107.

Serna-Escolano, V., Valverde, J. M., García-Pastor, M. E., Valero, D., Castillo, S., Guillén, F., Martínez-Romero, D., Zapata, P. J., & Serrano, M. (2019). Pre-harvest methyl jasmonate treatments increase antioxidant systems in lemon fruit without affecting yield or other fruit quality parameters. The Journal of the Science of Food and Agriculture, 99, 5035–5043. https://doi.org/10.1002/jsfa.9746.

Shah, H. M. A., Singh, Z., Hasan, M. U., Woodward, A., & Afrifa-Yamoah, E. (2025). Preharvest methyl jasmonate application delays cell wall degradation and upregulates phenolic metabolism and antioxidant activities in cold stored raspberries. Food Chemistry, 462, 141020. https://doi.org/10.1016/j.foodchem.2024.141020.

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158. https://doi.org/10.5344/ajev.1965.16.3.144.

Tulipani, S., Mezzetti, B., Capocasa, F., Bompadre, S., Beekwilder, J., de Vos, C. H., Capanoglu, E., Bovy, A., & Battino, M. (2008). Antioxidants, phenolic compounds, and nutritional quality of different strawberry genotypes. Journal of Agricultural and Food Chemistry, 56(3), 696-704. https://doi.org/10.1021/jf0719959.

Vithana, M.D.K., Singh, Z. & Ul Hasan, M. (2024). Pre- and post-harvest elicitation with methyl jasmonate and salicylic acid followed by cold storage synergistically improves red colour development and health-promoting compounds in blood oranges. Journal of Plant Growth Regulation, 43, 1657–1671. https://doi.org/10.1007/s00344-023-11212-8.

Wang, S.Y., & Lin, H.S. (2000). Antioxidant activity in fruits and leaves of blackberry, raspberry and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry 48, 140–146. https://doi.org/10.1021/jf9908345.

Wang, H., Wu, Y., Yu, R., Wu, C., Fan, G., & Li, T. (2019). Effects of postharvest application of methyl jasmonate on physicochemical characteristics and antioxidant system of the blueberry fruit. Scientia Horticulturae, 258, 108785. https://doi.org/10.1016/j.scienta.2019.108785.

Wolucka, B. A., Goossens, A., & Inzé, D. (2005). Methyl jasmonate stimulates the de novo biosynthesis of vitamin C in plant cell suspensions. Journal of Experimental Botany, 56(419), 2527–2538. https://doi.org/10.1093/jxb/eri246.

Wu, Y., Zhang, S., Yang, H., Wu, W., Lyu, L., Zhang, C., Cao, F., & Li, W. (2025). Methyl jasmonate and salicylic acid treatment changes the nutritional quality, antioxidant profile and gene expression of postharvest blackberry fruit. Postharvest Biology and Technology, 219, 13205. https://doi.org/10.1016/j.postharvbio.2024.113205.

Xu, J., Wang, Y., Chai, L., Yin, D., Lin, T., Tao, Y., Liu, S., Qi, H., Gao, X., & Jiang, J. (2024). Pre-harvest treatments: A different insight into preservation of strawberries. Journal of Berry Research, 14(2), 151-173. https://doi.org/10.3233/JBR-240001.

Yang, X., Liu, N., Tan, S., Xu, Y., Luo, Z., & Xie, G. (2025). Preharvest methyl jasmonate maintain the shelf quality of kiwifruit after cold storage by regulating the antioxidant system. Postharvest Biology and Technology, 221, 113335. https://doi.org/10.1016/j.postharvbio.2024.113335.

Zheng, H., Yang, Y., Wu, S., Jia, F., Jiang, J., Yu, L., Ou, G., Shu, M., & Qin, W. (2024). Effects of pre-harvest application of melatonin, 24-epibrassinolide, and methyl jasmonate on flavonoid content in blueberry fruit. Frontiers in Nutrition, 11, 1495655. https://doi.org/10.3389/fnut.2024.1495655.

Zuñiga, P. E., Castañeda, Y., Arrey-Salas, O., Fuentes, L., Aburto, F., & Figueroa, C. R. (2020). Methyl jasmonate applications from flowering to ripe fruit stages of strawberry (Fragaria × ananassa ‘Camarosa’) reinforce the fruit antioxidant response at post-harvest. Frontiers in Plant Science, 11, 538. https://doi.org/10.3389/fpls.2020.00538.

Influence of pre-harvest methyl jasmonate application on the fruit quality of strawberry cv. Paros at harvest and during cold storage

References

References

Articles in Press, Accepted Manuscript Available Online from 27 July 2025

Articles in Press, Accepted Manuscript
Available Online from 27 July 2025