Document Type : Original Article
Authors
1 Department of Horticulture Science, Faculty of Plant Production, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran
2 Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran
Abstract
Purpose: A study was carried out to investigate the influence of different fruit development stages on the physicochemical attributes, total phenolics, and antioxidant activity of two Iranian melon varieties for optimal harvest time. Research method: The research was conducted two times i.e. in 2023 and in 2024. It was a factorial experiment in a Completely Randomized Design (CRD) with four replications. The factors were developmental stage harvest (three stages, viz., 30, 40, and 50 days after fruit set) and variety (two varieties, viz., ‘Chaghercheh’ and ‘Zamcheh’). Findings: There were significant differences among the different harvest times in all parameters evaluated for both varieties. The fresh weight and related dimensions, and thickness and percentage of pulp of the fruits increased throughout the developmental stages, while fruit firmness, peel thickness, percentages of peel and seed showed a descending trend in the same period. At 50 days after fruit set, both melon varieties exhibited high levels of total soluble solids and pH, but low titratable acidity, resulting in high maturity index. As the fruits developed and matured, levels of ascorbic acid, total phenolics, and antioxidant activity reduced dramatically in both varieties. Also, statistically significant differences were detected between the two melon varieties in all factors measured. The antioxidant activity positively correlated with total phenolics (r = 0.910) and ascorbic acid (r = 0.891). Research limitations: No limitations were found. Originality/Value: Overall, the data of this research revealed that the degree of maturity and the variety are main parameters controlling the physicochemical characteristics, total phenolics, and antioxidant activity of melon. Also, the study provides basis for optimal harvest time for ‘Chaghercheh’ and ‘Zamcheh’ melon varieties.
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Ahmed, O. K. (2009). Evaluation of objective maturity indices for muskmelon (Cucumis melo) cv. "Galia". Journal of King Abdulazi University-Science, 21(2), 317-326. https://doi.org/10.4197/Sci.21-2.9
Aminah, A., & Anna, P. K. (2011). Influence of ripening stages on physicochemical characteristics and antioxidant properties of bitter gourd (Momordica charantia). International Food Research Journal, 18(3), 895-900.
AOAC. 2002. Official Methods of Analysis. 17th Edition, Association of Official Analytical Chemists, Arlington.
Arabia, A., Munne-Bosch, S., & Munoz, P. (2024). Ascorbic acid as a master redox regulator of fruit ripening. Postharvest Biology and Technology, 207, 112614. https://doi.org/10.1016/j.postharvbio.2023.112614
Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1), 191-203. https://doi.org/10.1016/j.foodchem.2005.07.042
Beaulieu, J. C., & Lea, J. M. (2007). Quality changes in cantaloupe during growth, maturation, and in stored fresh-cut cubes prepared from fruit harvested at various maturities. Journal of the American Society for Horticultural Science, 132(5), 720-728. https://doi.org/10.21273/JASHS.132.5.720
Bianchi, T., Guerrero, L., Gratacós-Cubarsí, M., Claret, A., Argyris, J., Gracia-Mas, J. & Hortós, M. (2016). Textural properties of different melon (Cucumis melo L.) fruit types: sensory and physical-chemical evaluation. Scientia Horticulturae, 201, 46-56. http://doi.org/10.1016/j.scienta.2016.01.028
Burger, Y., Paris, H. S., Cohen, R., Katzir, N., Tadmor, Y., Lewinsohn, E., & Schaffer, A. A. (2009). Genetic diversity of Cucumis melo. Horticultural Reviews, 35, 165-198. http://doi.org/10.1002/9780470527238.ch3
Burger, Y., Saar, U., Distelfeld, A., Katzir, N., Yeselson, Y., Shen, S., & Schaffer, A. A. (2003). Development of sweet melon (Cucumis melo) genotypes combining high sucrose and organic acid content. Journal of the American Society for Horticultural Science, 128(4), 537-540. http://doi.org/10.21273/JASHS.128.4.0537
Chisari, M., Silveira, A. C., Barbagallo, R. N., Spagna, G., & Arte´s, F. (2008). Ripening stage influenced the expression of polyphenol oxidase, peroxidase, pectin methylesterase and polygalacturonase in two melon cultivars. International Journal of Food Science and Technology, 44(5), 940-946. https://doi.org/10.1111/j.1365-2621.2008.01735.x
Dai, J., & Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15, 7313-7352. https://doi.org/10.3390/molecules15107313
Decros, G., Baldet, P., Beauvoit, B., Stevens, R., Flandin, A., Colombi´e, S., Gibon, Y., & P´etriacq, P. (2019). Get the balance right: ROS homeostasis and redox signalling in fruit. Frontiers in Plant Science, 10, 1091. https://doi.org/10.3389/fpls.2019.01091
Derong, L., Mengshi, X., Jingjing, Z., Zhuohao, L., Baoshan, X., Xindan, L., Maozhu, K., Liangyu, L., Qing, Z., Yaowen, L., Hong, C., Wen, Q., Hejun, W., & Saiyan, C. (2016). An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules, 21, 1374. https://doi.org/10.3390/molecules21101374
Ezzat, S. M., Raslan, M., Salama, M. M., Menze, E. T., & El Hawary, S. S. (2019). In vivo anti-inflammatory activity and UPLC-MS/MS profiling of the peels and pulps of Cucumis melo var. cantalupensis and Cucumis melo var. reticulatus. Journal of Ethnopharmacology, 237, 245-254. https://doi.org/10.1016/j.jep.2019.03.015
Falah, M. A. F., Khuriyati, N., Safitri, R. M., & Revulaningtyas, I. R. (2014). Quality evaluation of fresh and fresh-cut melon (Cucumis melo L) fruit in a tropical environment. International Journal of Agricultural Technology, 10(5), 1201-1211.
Fundo, J. F., Miller, F. A., Garcia, E., Santos, J. R., Silva, C. L. M., & Brandão, T. R. S. (2018). Physicochemical characteristics, bioactive compounds and antioxidant activity in juice, pulp, peel and seeds of cantaloupe melon. Journal of Food Measurement and Characterization, 12, 292-300. https://doi.org/10.1007/s11694-017-9640-0
Ghanbarian, D., Shojaei, Z. A., Ebrahimi, A., & Yuneji, S. (2007). Physical properties and compositional changes of two cultivars of cantaloupe fruit during various maturity stages. Iran Agricultural Research, 25(2), 117-125.
Guler, Z., Karaca, F., & Yetisir, H. (2013). Volatile compounds and sensory properties in various melons, which were chosen from different species and different locations, grown in Turkey. International Journal of Food Properties, 16(1), 168-179. https://doi.org/10.1080/10942 912.2010.528110
Ibrahim, S., Haidari, R. A., Mohamed, G., Elkhayat, E., & Moustafa, M. (2016). Cucumol A: a cytotoxic triterpenoid from Cucumis melo seeds. Brazilian Journal of Pharmacognosy, 26, 701-0704. http://dx.doi.org/10.1016/j.bjp.2016.03.012
Ismail, H. I., Chan, K. W., Mariod, A. A., & Ismail, M. (2010). Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts. Food Chemistry, 119, 643-647. https://doi.org/10.1016/j.foodchem.2009.07.023
Ismail, H. K., Mehmet, A., & Hacer, C. (2009). Antioxidant capacity, total phenolics and some chemical properties of semi matured apricot cultivars grown in Malatya, Turkey. World Applied Sciences Journal, 6(4), 519-523.
Kolayli, S., Kara, M., Tezcan, F., Erim, F. B., Sahin, H., Ulusoy, E., & Aliyazicioglu, R. (2010). Comparative study of chemical and biochemical properties of different melon cultivars: standard, hybrid, and grafted melons. Journal of Agricultural and Food Chemistry, 58, 9764-9769. https://doi.org/10.1021/jf102408y
Koubala, B. B., Bassang’na, G., Yapo, B. M., & Raihanatou, R. (2016). Morphological and biochemical changes during muskmelon (Cucumis melo var. tibish) fruit maturation. Journal of Food and Nutrition Sciences, 4(1), 18-28. https://doi.org/10.11648/j.jfns.20160401.14
Kumar, K., Debnath, P., Singh, S., & Kumar, N. (2023). An overview of plant phenolics and their involvement in abiotic stress tolerance. Stresses, 3, 570-585. https://doi.org/10.3390/stresses3030040
Li, F., Xu, B. T., Xu, X. R., Gan, R. Y., Zhang, Y., Xia, E. Q., & Li, H. B. (2011). Antioxidant capacities and total phenolic contents of 62 fruits. Food Chemistry, 129, 345-350. https://doi.org/10.1016/j.foodchem.2011.04.079
Li, J., Ren, D., Lin, T., Yan, W., Su, Z., Zhang, K., Xu, L., Ma, G., & Zhu, P. (2025). Gibberellin-dependent pulp pigmentation in CPPU-induced parthenocarpic melon fruit: insights from metabolome and transcriptome analysis. Scientia Horticulturae, 339, 113851. https://doi.org/10.1016/j.scienta.2024.113851
Li, M., Han, D., & Liu, W. (2019). Non-destructive measurement of soluble solids content of three melon cultivars using portable visible/near infrared spectroscopy. Biosystems Engineering, 188, 31-39. https://doi.org/10.1016/j.biosystemseng.2019.10.003
Machado, M., Felizardo, C., Fernandes-Silva, A. A., Nunes, F. M., & Barros, A. (2013). Polyphenolic compounds, antioxidant activity and l-phenylalanine ammonia-lyase activity during ripening of olive cv. “Cobrançosa” under different irrigation regimes. Food Research International, 51, 412-421. https://doi.org/10.1016/j.foodres.2012.12.056
Maietti, A., Tedeschi, P., Stagno, C., Bordiga, M., Travaglia, F., Locatelli, M., Arlorio, M., & Brandolini, V. (2012). Analytical traceability of melon (Cucumis melo var. reticulatus): proximate composition, bioactive compounds, and antioxidant capacity in relation to cultivar, plant physiology state, and seasonal variability. Journal of Food Science, 77, C646-C652. https://doi.org/10.1111/j.1750-3841.2012.02712.x
Mallek-Ayadi, S., Bahloul, N., Baklouti, S., & Kechaou, N. (2022). Bioactive compounds from Cucumis melo L. fruits as potential nutraceutical food ingredients and juice processing using membrane technology. Food Science and Nutrition, 10, 2922-2934. https://doi.org/10.1002/fsn3.2888
Manchali, S., Murthy, K. N. C., Vishnuvardana, & Patil, B. S. (2021). Nutritional composition and health benefits of various botanical types of melon (Cucumis melo L.). Plants, 10, 1755. https://doi.org/10.3390/plants10091755
Maruta, T., Sawa, Y., Shigeoka, S., & Ishikawa, T. (2016). Diversity and evolution of ascorbate peroxidase functions in chloroplasts: more than just a classical antioxidant enzyme. Plant and Cell Physiology, 57, 1377-1386. https://doi.org/10.1093/pcp/pcv203
Matsumura, Y., Kitabatake, M., Kayano, S., & Ito, T. (2023). Dietary phenolic compounds: their health benefits and association with the gut microbiota. Antioxidants, 12, 880. https://doi.org/10.3390/antiox12040880
McCollum, T. G., Huber, D. J., & Cantliffe, D. J. (1988). Soluble sugar accumulation and activity of related enzymes during muskmelon fruit development. Journal of the American Society of Horticultural Science, 113(3), 399-403. https://doi.org/10.21273/JASHS.113.3.399
Miccolis, V., & Saltveit, M. E. J. (1991). Morphological and physiological changes during Fruit growth and maturation of seven melon cultivars. Journal of the American Society of Horticultural Science, 116(6), 1025-1029. https://doi.org/10.21273/JASHS.116.6.1025
Miller. F. A., Fundo, J. F., Garcia, E., Santos, J. R., Silva, C. L. M., & Brandao, T. R. S. (2020). Physicochemical and bioactive characterisation of edible and waste parts of “Piel de Sapo” melon. Horticulturae. 6(60), 1-10. https://doi.org/10.3390/horticulturae6040060
Motomura, Y., Sugawara, J., Aikawa, T., Nara, K., & Nishizawa, T. (2018). Changes in free and bound phenolic contents and antioxidant activities of melon flesh dried at different stages of fruit growth. In Proceedings of the 3rd Asia Pacific Symposium on Postharvest Research, Education and Extension (APS), Ho Chi Minh City, Vietnam (pp. 505-510).
Munoz, P., & Munn´e-Bosch, S. (2018). Photo-oxidative stress during leaf, flower and fruit development. Plant Physiology, 176, 1004-1014. https://doi.org/10.1104/ pp.17.01127
Nishiyama, K., Guis, M., Rose, J. K., Kubo, Y., Bennett, K. A., Wangjin. L., Kato, K., Ushijima, K., Nakano, R., Inaba, A., Bouzayen, M., Latche, A., Pech, J-C., & Bennett, A. B. (2007). Ethylene regulation of fruit softening and cell wall disassembly in α1arentais melon. Journal of Experimental Botany, 58, 1281-1290. https://doi.org/10.1093/jxb/erl283
Pontes, F. M., Sarmento, J. D. A., Melo, N. J. D. A., Medeiros, E. V. D., Morais, P. L. D., & Nunes, G. H. D. S. (2021). Physical and chemical properties, pectinases activity, and cell wall pectin of acidulus, momordica, inodorus and cantalupensis melons with different ripening degree at harvest. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(2), 12062. https://doi.org/10.15835/nbha49212062
Rahman, M. M., Rahman Khan, M. M., & Hosain, M. M., (2007). Analysis of vitamin C (ascorbic acid) contents in various fruits and vegetables by UV-spectrophotometry. Bangladesh Journal of Scientific and Industrial Research, 42(4), 417-424. https://doi.org/10.3329/bjsir.v42i4.749
Seo, M. H., Tilahun, S., Park, D. S., Melaku, A., & Jeong, C. S. (2018). Effect of ripening conditions on the quality and storability of muskmelon (Cucumis melo L.) fruits. Horticultural Science and Technology, 36(5), 741-755. https://doi.org/10.12972/kjhst.20180073
Shebis, Y., Iluz, D., Kinel-Tahan, Y., Dubinsky, Z., & Yehoshua, Y. (2013). Natural antioxidants: function and sources. Food and Nutrition Sciences, 4(6), 643-649. https://doi.org/10.4236/fns.2013.46083
Singh, J., Metrani, R., Jayaprakasha, G. K., Crosby, K. M., Jifon, J. L., Ravishankar, S., Brierley, P., Leskovar, D. L., Turini, T. A., Schultheis, J., Coolong, T., Guan, W., & Patil, B. S. (2022). Profiling carotenoid and sugar contents in unique Cucumis melo L. cultigens harvested from different climatic regions of the United States. Journal of Food Composition and Analysis, 106, 104306. https://doi.org/10.1016/j.jfca.2021.104306
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. S. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteau reagent. Methods in Enzymology, 299, 152-178. http://doi.org/10.1016/S0076-6879(99)99017-1
Tomaz, H. V. D., Aroucha, E. M. M., Nunes, G. H. D., Neto, F. B., & Queiroz, R. F. (2009). Postharvest quality of different yellow melon hybrids stored under refrigeration. Revista Brasileira de Fruticultura, 31, 987-994. https://doi.org/10.1590/S0100-29452009000400011
Vaibhav, D. A., Arunkumar, W., Abhijit, M. P., & Arvind, S. (2011). Antioxidants as an immunomodulatory: an expanding research avenue. International Journal of Current Pharmaceutical Research, 1, 8-10.
Van Aken, O. (2021). Mitochondrial redox systems as central hubs in plant metabolism and signaling. Plant Physiology, 186, 36-52. https://doi.org/10.1093/plphys/kiab101
Vanoli, M., Cortellino, G., Picchi, V., Buccheri, M., Grassi, M., Lovat, F., Marinoni, L., Levoni, P., Torricelli, A., & Spinelli, L. (2023). Non-destructive determination of ripening in melon fruit using time-resolved spectroscopy. Advances in Horticultural Science, 37(1), 75-82. https://doi.org/10.36253/ahsc13943
Villanueva, M. J., Tenorio, M. D., Esteban, M. A., & Mendoza, M. C. (2004). Compositional changes during ripening of two cultivars of muskmelon fruits. Food Chemistry, 87, 179-185. https://doi.org/10.1016/j.foodchem.2003.11.009
Vishwakarma, V. K., Gupta, J. K., & Upadhyaya, P. K. (2017). Pharmacological importance of Cucumis melo L.: an overview. Asian Journal of Pharmaceutical and Clinical Research, 10(3), 8-12. http://dx.doi.org/10.22159/ajpcr.2017.v10i3.13849
Wang, X., Ando, K., Wu, S., Reddy, U. K., Tamang, P., Bao. K., Hammar. S. A., Grumet, R., McCreight, J. D., & Fei, Z. (2021). Genetic characterization of melon accessions in the U.S. National Plant Germplasm System and construction of a melon core collection. Molecular Horticulture, 1, 11. https://doi.org/10.1186/s43897-021-00014-9
Wang, Y., Gao, H., Guo, Z., Peng, Z., Li, S., Zhu, Z., Grimi, N., & Xiao, J. (2023). Free and bound phenolic profiles and antioxidant activities in melon (Cucumis melo L.) pulp: comparative study on six widely consumed varieties planted in Hainan province. Foods, 12, 4446. https://doi.org/10.3390/ foods12244446
Wheeler, G., Ishikawa, T., Pornsaksit, V., & Smirnoff, N. (2015). Evolution of alternative biosynthetic pathways for vitamin C following plastid acquisition in photosynthetic eukaryotes. Elife, 13, 4:e06369. https://doi.org/10.7554/eLife.06369
Zehiroglu, C., & Sarikaya, S. B. O. (2019). The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology, 56(11), 4757-4774. https://doi.org/10.1007/s13197-019-03952-x
Zhang, H., Pu, J., Tang, Y., Wang, M., Tian, K., Wang, Y., Luo, X., & Deng, Q. (2022). Changes in phenolic compounds and antioxidant activity during development of ‘Qiangcuili’ and ‘Cuihongli’ fruit. Foods, 11, 3198. https://doi.org/10.3390/foods11203198
Zhang, X., Bai, Y., Wang, Y., Wang, C., Fu, J., Gao, L., Liu, Y., Feng, J., Swamy, M. K., Yogi, M., Rudramurthy, G. R., Purushotham, B., & Deng, Y. (2020). Anticancer properties of different solvent extracts of Cucumis melo L. seeds and whole fruit and their metabolite profiling using HPLC and GC-MS. BioMed Research International, 24, 5282949. https://doi.org/10.1155/2020/5282949
Zheng, X., Gong, M., Zhang, Q., Tan, H., Li, L., Tang, Y., Li, Z., Peng, M., & Deng, W. (2022). Metabolism and regulation of ascorbic acid in fruits. Plants, 11(12), 1602. https://doi.org/10.3390/plants11121602
Zulfikar, M., Widya, F. S, Wibowo, W. A., Daryono, B. S., & Widiyanto, S. (2020). Antioxidant activity of melon fruit (Cucumis melo L. ‘GMP’) ethanolic extract. The 6th International Conference on Biological Science, https://doi.org/10.1063/5.0015748
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