A new protocol for direct regeneration of stevia plant (Stevia rebaudiana Bertoni) by tissue culture techniques

Rezaie, Soghra; Dehestani-Ardakani, Maryam; Kamali, Kazem

doi:10.22077/jhpr.2018.1273.1010

Document Type : Original Article

Authors

¹ Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran

² Department of Soil Science, Faculty of Natural Resources, Yazd University, Yazd, Iran

https://doi.org/10.22077/jhpr.2018.1273.1010

Abstract

Purpose: Due to pharmaceutical value of Stevia plant (Stevia rebudiana Bertoni.), this study was done to introduce a new protocol for rapid mass propagation of itthrough tissue culture. Research Method: In MS medium shoot proliferation of stevia by six concentrations of BA (0, 0.1, 0.2, 0.3, 0.4, 0.5 mg·l^-1) and root induction by four concentrations of IBA (0, 0.025, 0.05, 0.1 mg·l^-1) was investigated.Rooting of cuttings was done both in vitro and ex vivo conditions. Findings: According to the results, the most number of stems obtained in MS medium containing 0.3 mg l^-1BA. The highest length of stems obtained in MS medium without BA and the most number of leaves observed in MS medium supplemented with 0.4 mg·l^-1 BA. In in vitro situation, the most number and length of roots obtained in MS medium without IBA. The most number of rooted cuttings was obtained in IBA solution after 72 hours and about 70% of rooted cuttings were healthy. Research limitations: It had no limitation to report. Originality/value: In conclusion, it seems that the potential of producing root and shoots in stevia plant is extremely high, so its proliferation is possible using low concentrations of plant growth regulators in in vitro culture.

Keywords

Main Subjects

Biotechnology and Tissue culture

References

Abd Alhady, M.R.A. (2011). Micropropagation of Stevia rebaudiana Bertoni. A new sweetening crop in Egypt. Global Journal of Biotechnology & Biochemistry, 6(4), 178-182.

Aggarwal, A., Singh, N., & Yadav, K. (2011). Influence of arbuscular mycorrhizal (AM) fungi on survival and development of micropropagated Acorus calamus L. during acclimatization. International Journal of Agricultural Technology, 7(3), 775-781.

Ahmed, M.B., Salahin M., Karim, R., Razvy, M.A, Hannan, M.M., Sultana, R., Hossain, M., & Islam, R. (2007). An efficient method for in vitro clonal propagation of a newly introduced sweetener Plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Science and Research, 2, 121–125.

Altaf, T., Amin, S., Singh, S., & Kaloo, Z.A. (2013). Micropropagation of medicinally important plant species of family Asteraceae– a review. International Journal of Recent Scientific Research, 4(8), 1296- 1303.

Babber, S., Mittal, K., Ahlawat, R., & Varghese, T. (2001). Micropropagation of Cardiospermum halicacabum. Biologia Plantarum, 44, 603-606.

Borroto, J., Coll, J., Rivas, M., Blanco, M., Concepcion, O., Tandron, Y.A., Hernandez, M., & Trujillo, R. (2008) Anthraquinones from in vitro root culture of Morinda royoc L. Plant Cell Tissue and Organ Culture, 94,181–187.

Das, A., Gantait, S., & Mandal, N. (2011). Micropropagation of an Elite Medicinal Plant: Stevia rebaudiana Bert. International Journal of Agricultural Research, 6, 40-48.

Geuns, J.M.C. (2003). Molecules of interest stevioside. Phytochemistry, 64, 913–921.

Ibrahim, I.A., Nasr, M.I., Mohammed, B.R., & El-Zefzafi, M.M. (2008). Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Technology, 10, 254–259.

Jain, P., Kachhwaha, S., & Kothari, S.L. (2009). Improved micropropagation protocol and enhancement in biomass and chlorophyll content in Stevia rebaudiana (Bert.) Bertoni by using high copper levels in the culture medium. Scientia Horticulturae, 119, 315–319.

Kalpana, M., Anbazhagan, M., Natarajan, V., & Dhanavel, D. (2010). Improved micropropagation method for the enhancement of biomass in Stevia rebaudiana Bertoni. Recent Research on Science and Technology, 2, 8–13.

Majumder, S., & Mahbubur Rahman, M.D. (2016). Micropropagation of Stevia rebaudiana Bertoni. through direct and indirect organogenesis. Journal of Innovations in Pharmaceuticals and Biological Sciences, 3(3), 47-56.

Mathur, M., & Begum, T. (2015). Shootlets Regeneration and Tissue Culture Studies on Stevia rebaudiane Bertoni and Terminalia bellerica Roxb. International Journal of Research of Biotechnology, 3 (1), 25-35.

Modi, A.R., Patil, G., Kumar, N., Singh, A.S., & Subhash, N. (2012). A simple and efficient in vitro mass multiplication procedure for stevia rebaudiana Bertoni and analysis of genetic fidelity of in vitro raised plants through RAPD. Sugar Technology, 14, 391–397.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.

Ojha, A., & Chadhary, R. (2013). Callus development and indirect shoot regeneration from leaves and stem explants of Stevia rebaudiana Bertoni. Journal of Cell and Tissue Research, 13 (2), 3755-3759.

Sairkar, P., Chandravanshi, M.K., Shukla, N.P., & Mehrotra, N.N. (2009). Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. Journal of Medicinal Plants Research, 3(4), 266-270.

Sakaguchi, M., & Kan, T. (1982). Japanese researches on Stevia rebaudiana (Bert.) Bertoni and stevioside. CiCult, 34, 235-248.

Singh, S., Garg, V., Yadav, D., & Beg, M.N. (2012). In vitro antioxidative and antibacterial activities of various parts of Stevia rebaudiana Bertoni. International Journal of Pharmacy and Pharmaceutical Sciences. ISSN- 0975-149.

Singh, S.D., & Rao, G.P. (2005). Stevia: the herbal sugar of 21^st century. Sugar Technology, 7, 17–24.

Sivaram, L., & Mukundan, U. (2003). In vitro culture studies on (stevia rebaudiana). In vitro cellular Developmental and Biology plant, 39, 520- 523.

Toffler, F., & Orio, O.A. (1981). Acceni sulla pin ata tropicale ‘Kaa-he-e’ ou ‘erba dolce’. Revista. Society and Science Aliment, 4, 225-230 [English summary].

Tomaszewska-Sowa, M., Figas, A., & Katarzyna Sawilska, A. (2015). A non-caloric sweetener Stevia rebaudiana Bertoni- tissue culture plantlets for organic farming and home gardening.Infrastruktura i Ekologia Terenów Wiejskich, (IV/3).

Journal of Horticulture and Postharvest Research

A new protocol for direct regeneration of stevia plant (Stevia rebaudiana Bertoni) by tissue culture techniques

References

References

Volume 1, Issue 2 - Serial Number 2
September 2018
September 2018
Pages 97-104

A new protocol for direct regeneration of stevia plant (Stevia rebaudiana Bertoni) by tissue culture techniques

References

References

Volume 1, Issue 2 - Serial Number 2September 2018September 2018Pages 97-104

Volume 1, Issue 2 - Serial Number 2
September 2018
September 2018
Pages 97-104