An abiotic UV-B stress on Brassicaceae seeds increased their phytochemical content on 7-days sprouts

Martínez-Zamora, Lorena; Castillejo, Noelia; Gómez, Perla A.; Artés, Francisco; Artés-Hernández, Francisco

doi:10.22077/jhpr.2022.5406.1278

Document Type : Original Article

Authors

¹ Postharvest and Refrigeration Group. Department of Agricultural Engineering and Institute of Plant Biotechnology. Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48. 30203 Cartagena, Murcia, Spain

² Department of Food Technology, Food Science, and Nutrition, Faculty of Veterinary Sciences, University of Murcia, Espinardo, 30071 Murcia, Spain

https://doi.org/10.22077/jhpr.2022.5406.1278

Abstract

Purpose: Cruciferous sprouts in their early stages of development are very suitable foods against degenerative diseases due to their high content of health promoting compounds. The application of UV-B can act as an elicitor of these compounds. The objective was to study the effect of a UV-B treatment to different seed varieties and its remnant effects after germination as sprouts. Research method: Brassicaceae seeds selected (radish, rocket, white mustard, and tatsoi) were treated under 20 kJ m^-2 UV-B 24 h before sowing, while no UV radiation was used as control (CTRL). After 7 days, sprouts were harvested, frozen, and freeze-dried until their bioactive content was analysed. Findings: Results showed that UV-B enhanced by ~20% the biosynthesis of phenolic compounds in sprouts, and hence, their total antioxidant capacity. Furthermore, this UV effect was also appreciated after 7 germination days, and it was increased by ~38% regarding CTRL, especially on rocket and mustard sprouts. In conclusion, an abiotic UV-B stress treatment to seeds can be an interesting tool to improve the bioactive compounds content of young plants, although the intrinsic mechanisms involved should be further investigated. Research limitations: The use of these new technologies, such as UV-B, is costly and must be applied following appropriate safety measures to avoid possible irradiation damage. Originality/Value: The analysis of the remnant effect of the UV-B before seeds sprouting has not been yet studied and its use could result in a beneficial effect on the germination and biosynthesis of phytochemicals in young plants.

Graphical Abstract

Keywords

Main Subjects

Olericulture

References

Castillejo, N., Martínez-Hernández, G. B., Monaco, K., Gómez, P. A., Aguayo, E., Artés, F., & Artés-Hernández, F. (2017). Preservation of bioactive compounds of a green vegetable smoothie using short time-high temperature mild thermal treatment. Food Science and Technology International, 23(1), 46–60. https://doi.org/10.1177/1082013216656240

Castillejo, N., Martínez-Zamora, L., & Artés–Hernández, F. (2021). Periodical Uv-B Radiation Hormesis in Biosynthesis of Kale Sprouts Nutraceuticals. Plant Physiology and Biochemistry, 165, 274–285. https://doi.org/10.1016/j.plaphy.2021.05.022

Cloix, C., Kaiserli, E., Heilmann, M., Baxter, K. J., Brown, B. A., O’Hara, A., Smith, B. O., Christie, J. M., & Jenkins, G. I. (2012). C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein. Proceedings of the National Academy of Sciences of the United States of America, 109(40), 16366–16370. https://doi.org/10.1073/pnas.1210898109

Di Gioia, F., Leoni, B., & Santamaria, P. (2015). La scelta delle specie da coltivare. The selection of the species to grow. La elección de las especies a cultivar. In F. Di Gioia & P. Santamaria (Eds.), Microgreens: Novel, fresh and functional food to explore all the value of biodiversity. (1st ed., pp. 25–40). Eco-logica srl. http://www.gustailbiodiverso.com/wp-content/uploads/2015/11/Microgreens.pdf

Di Gioia, Renna, M., & Santamaria, P. (2017). Sprouts, Microgreens and “Baby Leaf” Vegetables. In F. Yildiz & R. C. Wiley (Eds.), Minimally Processed Refrigerated Fruits and Vegetables (2nd ed., pp. 403–432). Springer US. https://doi.org/10.1007/978-1-4939-7018-6_11

Escobar-Bravo, R., Klinkhamer, P. G. L., & Leiss, K. A. (2017). Interactive effects of UV-B light with abiotic factors on plant growth and chemistry, and their consequences for defense against arthropod herbivores. Frontiers in Plant Science, 8(March), 1–14. https://doi.org/10.3389/fpls.2017.00278

Falcone Ferreyra, M. L., Rius, S. P., & Casati, P. (2012). Flavonoids: Biosynthesis, biological functions, and biotechnological applications. Frontiers in Plant Science, 3(222), 1–15. https://doi.org/10.3389/fpls.2012.00222

Hamed, M., Kalita, D., Bartolo, M. E., & Jayanty, S. S. (2019). Capsaicinoids, polyphenols and antioxidant activities of Capsicum annuum: Comparative study of the effect of ripening stage and cooking methods. Antioxidants, 8(9), 364. https://doi.org/10.3390/antiox8090364

Jenkins, G. I. (2009). Signal transduction in responses to UV-B radiation. Annual Review of Plant Biology, 60, 407–431. https://doi.org/10.1146/annurev.arplant.59.032607.092953

Jenkins, G. I. (2014). The UV-B photoreceptor UVR8: From structure to physiology. Plant Cell, 26(1), 21–37. https://doi.org/10.1105/tpc.113.119446

Li, F. M., Lu, Z. G., & Yue, M. (2014). Analysis of photosynthetic characteristics and UV-B absorbing compounds in mung bean using UV-B and red LED radiation. Journal of Analytical Methods in Chemistry, 2014, 378242. https://doi.org/10.1155/2014/378242

Martínez-Zamora, L., Castillejo, N., & Artés-Hernández, F. (2021a). Postharvest UV-B and UV-C radiation enhanced the biosynthesis of glucosinolates and isothiocyanates in Brassicaceae sprouts. Postharvest Biology and Technology, 181, 111650. https://doi.org/10.1016/j.postharvbio.2021.111650

Martínez-Zamora, L., Castillejo, N., & Artés–Hernández, F. (2021b). UV-B radiation as abiotic elicitor to enhance phytochemicals and development of red cabbage sprouts. Horticulturae, 7(567), 1–18. https://doi.org/10.3390/ horticulturae7120567

Martínez-Zamora, L., Castillejo, N., & Artés–Hernández, F. (2022). Ultrasounds and a postharvest photoperiod to enhance the synthesis of sulforaphane and antioxidants in rocket sprouts. Antioxidants, 11(8), 1490. https://doi.org/10.3390/ antiox11081490

Martínez-Zamora, L., Castillejo, N., Gómez, P. A., & Artés-Hernández, F. (2021c). Amelioration effect of led lighting in the bioactive compounds synthesis during carrot sprouting. Agronomy, 11(2), 1–17. https://doi.org/10.3390/agronomy11020304

Morales, L. O., Tegelberg, R., Brosché, M., Keinänen, M., Lindfors, A., & Aphalo, P. J. (2010). Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves. Tree Physiology, 30(7), 923–934. https://doi.org/10.1093/treephys/tpq051

Peykarestan, B., Seify, M., Fadaei, M. S., & Hatim, M. (2012). UV irradiation effects on seed germination and growth, protein content, peroxidase and protease activity in Portulaca grandiflora and Portulaca oleracea. World Applied Sciences Journal, 17(7), 802–808.

Schreiner, M., Martínez-Abaigar, J., Glaab, J., & Jansen, M. (2014). UV-B Induced Secondary Plant Metabolites. Optik & Photonik, 9(2), 34–37. https://doi.org/10.1002/opph.201400048

Shubha, K., Mukherjee, A., Tamta, M., & Koley, T. K. (2019). Arugula (Eruca vesicaria subsp. sativa (Miller) Thell.): A healthy leafy vegetable. Agriculture & Food: E-Newsletter, 1(11), 359–362. https://doi.org/10.13140/RG.2.2.22117.35041

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3), 144–158.

Solovchenko, A. E., & Merzlyak, M. N. (2008). Screening of visible and UV radiation as a photoprotective mechanism in plants. Russian Journal of Plant Physiology, 55(6), 719–737. https://doi.org/10.1134/S1021443708060010

Taiz, L., & Zeiger, E. (2018). Plant physiology and Development. Annals of Botany, 91(6), 750–751. https://doi.org/10.1093/aob/mcg079

Journal of Horticulture and Postharvest Research

An abiotic UV-B stress on Brassicaceae seeds increased their phytochemical content on 7-days sprouts

References

References

Volume 5, Issue 4 - Serial Number 19
December 2022
December 2022
Pages 297-308

An abiotic UV-B stress on Brassicaceae seeds increased their phytochemical content on 7-days sprouts

References

References

Volume 5, Issue 4 - Serial Number 19December 2022December 2022Pages 297-308

Volume 5, Issue 4 - Serial Number 19
December 2022
December 2022
Pages 297-308