Salinity tolerance evaluation of twelve selected pomegranate (Punica granatum) genotypes to achieve tolerant cultivars and rootstocks

Momenpour, Ali; Dehestani Ardakani, Maryam; Shirmardi, Mostafa; Gholamnezhad, Jalal; Ahmadi, Fatemeh; Jamaati, Zahra

doi:10.22077/jhpr.2022.5152.1270

Document Type : Original Article

Authors

¹ National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran

² Department of Horticultural Science, College of Agriculture & Natural Resources, Ardakan University, Yazd, Iran

https://doi.org/10.22077/jhpr.2022.5152.1270

Abstract

Purpose: Pomegranate (Punica granatum L.) is a very interesting fruit tree for arid and semiarid areas in any part of the world. Like other fruit trees, the selection of tolerant rootstocks and scion is a very good strategy to reduce the adverse effects of salinity on pomegranate. Therefore, this study aimed to evaluate the effect of salinity stress on the growth characteristics of some selected pomegranate genotypes and introduce the most tolerant genotype(s) to salinity for use as a basis in future research. Research method: Selected pomegranate genotypes were evaluated using a factorial experiment based on a completely randomized design (CRD) with four replications in 2019-2020. Treatments were included 12 genotypes of Golanr-e-Shahdad (G-Shahdad), Golanr-e-Sarvestan(G-Sarvestan), Golnar-Saveh(G-Saveh), Poostsiah-e-Ardekan(Poostsiah), ‘Malas-e-Yazdi’(‘M-Yazdi’), ‘Malas-e-Saveh’(‘M-Saveh’), ‘Shishecap-e-Ferdos’ (‘Shishecap’), ‘Rabab-e-Neiriz’ (‘Rabab’), ‘Vahshi-e-Babolsar’(‘V-Babolsar’), ‘Narak-e-Lasjerd-Semnan’(‘Narak’), Chahafzal and ‘Voshik-e-Torsh-e-Saravan’(‘Voshik’) and the salinity of the irrigation water in five levels (1, 3, 5, 7 and 9 dS.m^-1). Findings: According to the results, the type of genotype and the level of salinity were affected on morphological and physiological traits as well as the concentration of nutrient elements. In all genotypes, the growth indices, relative water content (RWC), chlorophyll index, and total chlorophyll reduced as a result of increasing the salinity level. But the percentage of necrotic leaves, percentage of fallen leaves, ion leakage, concentration of Na⁺, the concentration of Cl^- and Na⁺/K⁺ ratio increased. At salinity level of 7dS.m^-1, necrotic leaves (3.11% &23.98%), fallen leaves (1.05% & 5.70%), ion leakage (5.87% & 22.10%), Na⁺(0.31% & 1.29%), concentration of Cl^-(0.13%& 1.10%), concentration of K⁺(0.64% & -0.07%) and Na⁺/K⁺ ratio (0.09 & 2.28 units) increased in Chahafzal and Voshik genotypes, respectively. Research limitations: No limitations to report. Originality/Value: ‘Chahafzal’ and ‘Poostsiah’ genotypes were recognized as the most tolerant to salinity according to the results. In contrast, Voshik and M-Saveh genotypes were more sensitive to salinity. The tolerant genotypes will be used in plans as rootstocks to graft the selected genotypes on them.

Keywords

Main Subjects

Plant Stress

References

Almeida, M. D., Oliveira, M. M., & Saibo. N. J. M. (2017). Regulation of Na+ and K+ homeostasis in plants: towards improved salt stress tolerance in crop plants. Genetics and Molecular Biology, 40, 326–345. https://doi.org/10.1590/1678-4685-gmb-2016-0106

Araújo, W. L., Fernie A. R., & Nunes-Nesi A. (2011). Control of stomatal aperture: a renaissance of the old guard. Plant Signaling and Behavior, 6, 1305–1311. https://doi.org/10.4161/psb.6.9.16425

Arnon, D. I. (1949). Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant Physiology, 24, 1- 15.

Ashraf, M. (1994). Organic substances responsible for salt tolerance in Eruca sativa. Biologia. Plantarum, 36, 255-259, https://doi.org/10.1007/BF02921095

Ashraf, M., & Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. – Plant Science, 166, 3-16. https://doi.org/10.1016/j.plantsci.2003.10.024

Calzone, A., Cotrozzi, L., Pellegrini, E., Guidi, L., Lorenzini, G., & Nali, C. (2020). Differential response strategies of pomegranate cultivars lead to similar tolerance to increasing salt concentrations. Scientia Horticulturae, 271, 109441. https://doi.org/10.1016/j.scienta.2020.109441

Dichala, O., Therios, I., Papadopoulos, A., Chatzistathis, T., Chatzisavvidis, C., & Antonopoulou, C. (2021). Effects of varying concentrations of different salts on the mineral composition of leaves and roots of three pomegranates (Punica granatum L.) cultivars. Scientia Horticulturae, 275, 109718. https://doi.org/10.1016/j.scienta.2020.109718

Emami, A. (1996). Methods of plant analysis. Agricultural Research and Education Organization. Soil and Water Institute. 130 Pp.

Flowers, T. J. & Colmer, T. D. (2015). Plant salt tolerance: adaptations in halophytes. Annals of Botany, 115, 327–331. https://doi.org/10.1093/aob/mcu267.

Guo, F. O., & Tang, Z. C. (1999). Reduced Na+ and K⁺ permeability of K⁺ channel in plasma membrane isolated from roots of salt-tolerant mutant of wheat. Chinese Academy of Sciences, 41(9), 217-220.

Ibrahim, H.I.M. 2016. Tolerance of two pomegranates cultivars (Punica granatum L.) to salinity stress under hydroponic culture conditions. Journal of Basic Applied Science Research Flora, 4, 38-46.

Karimi, H.R. & Z. Hasanpour. 2014. Effects of salinity and water stress on growth and macronutrient concentration of pomegranate (Punica granatum L.). Journal Plant Nutrition, 37, 1937–1951. https://doi.org/10.1080/01904167.2014.920363.

Khayyat, M., Tehranifar, A., Davarynejad, G.H., & Sayyari-Zahan, M.H. (2014). Vegetative growth, compatible solute accumulation, ion partitioning and chlorophyll fluorescence of ‘Malas-e-Saveh’ and ‘Shishe-Kab’ pomegranates in response to salinity stress. Photosynthetica, 52(2), 301-312. https://doi.org/10.1007/s11099-014-0034-9.

Liu, C., Ming, Y., Xianbin, H., & Zhaohe, Y. (2018). Effects of salt stress on growth and physiological characteristics of pomegranate (Punica granatum L.) cuttings. Pakistan Journal of Botany, 50 (2), 457-464.

Lutts, S., Kinet, J.M., & Bouharmont, J. (1995). Changes in plant response to NaCl during the development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46, 1843–1852. https://doi.org/10.1093/jxb/46.12.1843

Mahajan, Sh., & Tuteja, N. (2005). Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444, 139-158. https://doi.org/10.1016/j.abb.2005.10.018

Marschner, H. (1995). Mineral Nutrition of Higher Plants. 2nd Ed. Pp.892. Academic Press Ltd., London 1995.

Massai, R., Remorni, D., & Tattini, M. (2004). Gas exchange, water relations and osmotic adjustment in two scion/rootstock combinations of Prunus under various salinity concentrations. Journal of Plant Soil Science, 259, 153-162. https://www.jstor.org/stable/24124369

Mastrogiannidou, E., Chatzissavvidis, C., Antonopoulou, C., Tsabardoukas, V., Giannakoula, A., & Therios, I. (2016). Response of pomegranate cv. wonderful plants tο salinity. Journal of Soil Science and Plant Nutrition, 16(3), 621-636. http://dx.doi.org/10.4067/S0718-95162016005000032.

Momenpour, A., & Imani, A. (2018). Evaluation of salinity tolerance in fourteen selected pistachio (Pistacia vera L.) cultivars. Advances in Horticultural Science, 32 (2), 249-264. https://doi.org/10.13128/ahs-22261

Momenpour, A., Imani, A., Bakhshi, D., & Akbarpour, E. (2018). Evaluation of salinity tolerance of some selected almond genotypes budded on GF₆₇₇ rootstock. International Journal of Fruit Science, 18(4), 410-435. https://doi.org/10.1080/15538362.2018.1468850

Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell & Environment, 25, 239-250. https://doi.org/10.1046/j.0016-8025.2001.00808.x.

Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681. https://doi.org/10.1146/annurev.arplant.59.032607.092911.

Munns, R., James, R.A., & Lauchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57, 1025-1043. https://doi.org/10.1093/jxb/erj100

Naeini, M. R., Khoshgoftarmanesh, A. H., & Fallahi, E. (2006a). Partitioning of chlorine, sodium, and potassium and shoot growth of three pomegranate cultivars under different levels of salinity. Journal Plant Nutrition, 29, 1835-1843. https://doi.org/10.1080/01904160600899352.

Naeini, M. R., Khoshgoftarmanesh, A. H., Lessani, H., & Fallahi, E. (2006b). Effects of sodium chloride-induced salinity on mineral nutrients and soluble sugars in three commercial cultivars of pomegranate. Journal Plant Nutrition, 27, 1319-1326. https://doi.org/10.1081/PLN-200025832

Okhovatian-Ardakani, A. R., Mehrabanian, M., Dehghani, F., & Akbarzadeh, A. (2010). Salt tolerance evaluation and relative comparison in cuttings of different pomegranate cultivars. Plant Soil and Environment, 56(4), 176–185. https://doi.org/10.17221/158/2009-PSE

Pang, C. H., & Wang, B. S. (2008). Oxidative stress and salt tolerance in llants. In: In: Lüttge, U., Beyschlag, W., Murata, J. (Eds.), Progress in Botany, vol. 69. Springer, Berlin, pp. 231–245. https://doi.org/10.1007/978-3-540-72954-9_9.

Papadakis, I. E., Veneti, G., Chatzissavvidis, C., Sptiropoulos, T. E., Dimassi, N., & Therios, I. (2007). Growth, mineral composition, leaf chlorophyll and water relationships of two cherry varieties under NaCl-induced salinity stress. Soil Science and Plant Nutrition, 53, 252-258. https://doi.org/10.1111/j.1747-0765.2007.00130.x

Parvizi, H., Sepaskhah, A. R., & Ahmadi, S. H. (2016). Physiological and growth responses of pomegranate tree (Punica granatum (L.) cv. Rabab) under partial root-zone drying and deficit irrigation regimes. Agricultural Water Management, 163, 146–158. https://doi.org/10.1016/j.agwat.2015.09.019

Sarkhosh, A., Zamani, Z., , Fatahi, R., & . Ebadi, A. (2006). RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Journal of Horticultural Science, 111, 24–29. https://doi.org/10.1016/j.scienta.2006.07.033

Shibli, R. A., Shatnawi, M. A., & Swaidat, I. Q. (2003). Growth, osmotic adjustment and nutrient acquisition of bitter almond under induced sodium chloride salinity in vitro. Communication in Soil Science and Plant Analysis, 34, 1969-1979. https://doi.org/10.1081/CSS-120023231

Silva-Ortega, C.O., Ochoa-Alfaro, A.E., & Reyes-Aguerro, J.A. (2008). Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear. Plant Physiology and Biochemistry, 46, 82-92. https://doi.org/10.1081/CSS-120023231

Szczerba, M. W., Britto, D. T., & Kronzucker, H. J. (2009). K⁺ transport in plants: physiology and molecular biology. Journal of Plant Physiology, 166, 447-466. DOI: 10.1016/j.jplph.2008.12.009

Szczerba, M. W., Britto, D. T., Balkos, K. D., & Kronzucker, H. J. (2008). NH₄⁺ stimulated and -inhibited components of K+ transport in rice (Oryza sativa L.). Journal of Experimental Botany, 59, 3415–3423. https://doi.org/10.1093/jxb/ern190

Tester, M., & Davenport, R. (2003). Na+ tolerance and Na+ transport in higher plants. Annals of Botany, 91, 503–527. https://doi.org/10.1093/aob/mcg058.

Yamasaki, S., & Dillenburg, L. C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasileira de Fisiologia Vegetal, 11, 69-75.

Journal of Horticulture and Postharvest Research

Salinity tolerance evaluation of twelve selected pomegranate (Punica granatum) genotypes to achieve tolerant cultivars and rootstocks

References

References

Volume 5, Issue 4 - Serial Number 19
December 2022
December 2022
Pages 363-378

Salinity tolerance evaluation of twelve selected pomegranate (Punica granatum) genotypes to achieve tolerant cultivars and rootstocks

References

References

Volume 5, Issue 4 - Serial Number 19December 2022December 2022Pages 363-378

Volume 5, Issue 4 - Serial Number 19
December 2022
December 2022
Pages 363-378