Postharvest Biology and Technology
Farid Moradinezhad; Azam Naeimi; Homayoun Farhangfar
Abstract
Jujube fruits have a short shelf life due to its perishable nature. Preservation of jujube quality is a big challenge. Edible coatings have been used for centuries in the food industry as an effective method to preserve food products, enhance shelf life and prevent loss of firmness and moisture in fresh ...
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Jujube fruits have a short shelf life due to its perishable nature. Preservation of jujube quality is a big challenge. Edible coatings have been used for centuries in the food industry as an effective method to preserve food products, enhance shelf life and prevent loss of firmness and moisture in fresh fruits. However, little information is available on the overall quality attributes of Chinese jujube fruits influenced by edible coatings. Hence, the influence of different edible coatings including aloe vera gel (AV) (33 and 50% v/v), carboxymethyl cellulose (CMC) and pectin (1, 1.5 and 2% w/v) was studied on fresh jujube quality during 40 days of refrigerated storage at 4 °C. The results showed that weight loss was approximately reduced by 30% in coated jujubes with Aloe vera 33% v/v or 1.5% w/v pectin after 40 days compared to uncoated control. Uncoated fruits presented a higher and significant decay after 40 days of storage than other coating treatments. Results also indicated that the coatings with 50% v/v Aloe vera, 1 and 2% w/v CMC caused a higher and significant TA in jujube fruits, and preserved sensory acceptability as well as improved appearance quality of fruits compared to uncoated fruit. In general, coated fruit with Aloe vera at both concentrations and CMC at 1 and 1.5% after 40 days of refrigerated storage maintained the quality of jujube fruits better than control.
Tropical Fruits
Aurelice Oliveira; Monica Maria Lopes; Kellina Oliveira de Souza; Luciana de Siqueira Oliveira; Carlos Farley Moura; Maria Raquel de Miranda
Abstract
Purpose: The objective of this work was to evaluate the effects of two systems of cultivation on the banana crop (Musa spp.) in the postharvest quality, bioactive compounds and phenylalanine ammonia-lyase during ripening process. Research method: Changes in physicochemical parameters, non-antioxidant, ...
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Purpose: The objective of this work was to evaluate the effects of two systems of cultivation on the banana crop (Musa spp.) in the postharvest quality, bioactive compounds and phenylalanine ammonia-lyase during ripening process. Research method: Changes in physicochemical parameters, non-antioxidant, phenylalanine ammonia lyase (PAL) activity and oxidative markers were evaluated in banana cv. Prata-Anã from organic and conventional systems at three ripening stages: unripe, breaker and ripe. Main findings: The weight of conventional fruit was 48% greater at the ripe stage. The fruit size was reduced in fruits from organic farming while titratable acidity and the soluble solids content were respectively 82% and 58% higher at breaker stage in conventional bananas. The organic bananas have an increase of 58% in the phenolics at the unripe stage. The PAL activity was observed throughout banana development from organic farming, however the same was not observed for the conventional farming. Dismutase superoxide activity was also dramatically higher in matures and ripe fruits from organic farming. The lipid peroxidation degree of the cell membrane was 40% higher in ripe bananas for both systems. Research limitations: No limitations were founded, since the methods were well established. Originality/Value: Our observations suggest that banana fruits presented little changes in the function of farming conditions with an accumulation of specific compounds in determined stages of ripening without remarkable difference among systems of cultivation.
Postharvest Biology and Technology
Lisa Kitinoja; Olubukola Odeyemi; Neeru Dubey; Solange Musanase; Gurbinder Singh Gill
Abstract
Purpose: This study was conducted to identify and quantify the main causes and sources of losses in the tomato postharvest chain from harvest to retail market and identify appropriate interventions for reducing these losses in Nigeria, Rwanda and India. Research method: Modified Commodity Systems ...
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Purpose: This study was conducted to identify and quantify the main causes and sources of losses in the tomato postharvest chain from harvest to retail market and identify appropriate interventions for reducing these losses in Nigeria, Rwanda and India. Research method: Modified Commodity Systems Assessment Methodology on tomato was conducted in the study area during the July - August 2017 harvest season. Findings: Generally, production is increasing with high postharvest losses. Tomato postharvest losses were uniformly high on the farm during harvest but generally lower during marketing in India than in Rwanda or Nigeria. Nigeria loses 10-40% of tomato produced from the farm to the retail market due to poor handling and unavailability of storage facilities. In Rwanda, tomato losses were exceedingly high, reaching 50 to 60%. These losses begin with the use of poor quality seeds to rough handling and use of inappropriate packaging materials. Losses in India varied between 1-18% mainly due to pest and disease attack and low price realization during glut season. However, in India, improved practices have been widely adopted on farm to retail market. Limitations: Resources were limited, so a single two week time period were randomly selected to conduct each of the studies which focused on one state, district or region, so data cannot be used to describe losses for the entire country. Originality/Value: These studies identified priority tomato postharvest problems, key issues that currently limit market access, earnings for small farmers and rural marketer, training and advocacy issues for the three countries.
Biotechnology and Tissue culture
Mariem Lotfi; Chokri Bayoudh; Afifa Majdoub; Messaoud Mars
Abstract
Purpose: In Tunisia, pear cultivars are widely threatened by the attack of fire blight disease. Cultivation of tolerant cultivars is an effective control strategy for disease control. For this purpose, a reliable protocol was established for micropropagation of local Pyrus communis and Pyrus syriaca ...
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Purpose: In Tunisia, pear cultivars are widely threatened by the attack of fire blight disease. Cultivation of tolerant cultivars is an effective control strategy for disease control. For this purpose, a reliable protocol was established for micropropagation of local Pyrus communis and Pyrus syriaca L. and for large-scale production of high-quality plantlets. Research method: Using apical explants, different media and hormones were tested to establish a micropropagation procedure for local Tunisian Pyrus communis cultivars ‘Arbi’, ʻMaltiʼ, ʻMahdia 6ʼ and ʻMoknine 10ʼ and for Pyrus syriaca. Disinfection with 4% HgCl2 treatment for 20 minutes showed the highest percentage of plant survival. Successful initiation of the cultures was achieved on MS basal medium supplemented with 0.25 mg L-1 BA. Findings: During the proliferation stage, optimal shoot multiplication was obtained on MS medium with a half concentration of NH4NO3 and KNO3 supplemented with 0.1 mg L-1 IBA and 2 mg L-1 BA, but for maximum shoot length the BA concentration needed to be lowered to 1 mg L-1. A rooting rate of 100% and the highest root length and root number were attained on Cheng medium supplemented with 1.0 mg L-1 IBA. Pear vitroplants were successfully acclimatized on S2 substrate, composed by peat moss. Research limitations: Vitroplants acclimatization step needs to be well studied for the improvement of theacclimatized vitroplant survival rates by reducing the symptoms of crown rot. Originality/Value: This efficient optimized in vitro protocol will be successfully applied for large multiplication of high quality of Tunisian Pyrus vitroplants and cultivars.
Plant Stress
Simeneh Tamrat Alemu; Amsalu Gobena Roro
Abstract
Purpose: herbaceous dicot plants mainly sweet potato is highly affected by UV-B and light quality based on altitude. Therefore, this study will give insights on the effect of UV-B and light quality on photosynthetic efficiency of sweet potato based on Altitude. Research method: The experimental design ...
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Purpose: herbaceous dicot plants mainly sweet potato is highly affected by UV-B and light quality based on altitude. Therefore, this study will give insights on the effect of UV-B and light quality on photosynthetic efficiency of sweet potato based on Altitude. Research method: The experimental design was laid out in split plot design with factorial arrangement. The treatments consist of +UV-B or control, UV-B exclusion, UV-B+end of day light exclusion, and the two sweet potato cultivars such as Kulfo and Hawassa-83. Main findings: highest UV-B (1693.0mw/m-2s-2) was recorded at highland (2230m.a.s.l) and the lowest (1107mw/m-2s-2) was recorded at lowland (1400m.a.s.l). The result shows that photosynthetic rate increased by 2.41% in Kulfo compared with Hawassa83 cultivar. Also altitude has a highly significant effect on photosynthetic efficiency; the highest stress level 0.68 and 0.72 was recorded at UV-B + EOD exclusion and highland. This indicate UV-B +EOD exclusion cause light quality and intensity stress at highland also UV-B was inducing high stress at highland area and affecting sweet potato productivity but exclusion increase Fv/Fm and stomata conductance it has no effect on photosynthesis and transpiration rate. Limitations: The research has a limitation due to Altitude difference it’s difficult to control factors other than UV-B and light Quality that may cause a difference. Originality/Value: This research tries to assess UV-B and end of day time light quality effects due to altitude difference and encourages new research on UV-B, its adaptation and light quality on crop productivity.
Plant Allelopathy
A K M Mominul Islam; Md Azhiat-ul-Haque Hia; Md Anwarul Abedin; Md Jewel Alam; Md Parvez Anwar; A K M Aminul Islam
Abstract
Purpose: The experiment was conducted to investigate into the allelopathic potential of the aqueous extracts of different parts of Jatropha curcuson seven selected field crop species. Research method: The leaf, stem, bark, twig, root, pericarp, seed and oilcake extracts of J. curcus at four different ...
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Purpose: The experiment was conducted to investigate into the allelopathic potential of the aqueous extracts of different parts of Jatropha curcuson seven selected field crop species. Research method: The leaf, stem, bark, twig, root, pericarp, seed and oilcake extracts of J. curcus at four different concentrations (1:5, 1:10, 1:15 and 1:20 (w/v)) were tested against jute, mungbean, mustard, radish, rice, wheat and tomato. Control i.e. distilled water without extracts was also maintained in each case. The experiments were conducted following completely randomized design with three replications. Findings: Except few, the aqueous extracts of J. curcas plant parts significantly inhibited the seedling growth of all the test species at concentration more than 1:15 (w/v) whereas, at or below this level stimulated the seedling growth. Percent shoot and root growth inhibition of the test crops varied among J. curcus parts extract from 10 to 100, at 1:5 (w/v) concentration. At the same concentration, J. curcas oilcake extract completely (100%) inhibited the shoot and root growth of all the test crop species except rice. Similarly, seed extract completely inhibited (100%) the shoot and root growth of jute and mustard, whereas around or more than 70% inhibition of the shoot and root growth of all the test species except rice. These results confirm that J. curcushas allelopathic properties and may possess allelochemicals. Since oilcake of J. curcus extract had greater inhibitory activity than other parts, this could be used for isolation and identification of allelochemical(s). The results of this experiment will be helpful for the researchers to know the plant-plant interaction of J. curcus with its neighboring plant species or the intercrops introduce in Jatropha field. Limitations: There was no significant limitation to the report. Originality/Value: This research compares the allelopathic properties of different parts of Jatropha curcus on seven selected field crop species.
Fresh-Cut Produce
Anh T. Huynh; Ha V. H. Nguyen
Abstract
Purpose: This investigation focused on the most suitable temperature for fresh- cut pumpkin with harmonization between benefits and drawbacks. Research method: Fresh-cut pumpkin cubes were pre–treated with 30% ethanol and 1% chitosan, then stored for 15 days at different temperatures (5 °C ...
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Purpose: This investigation focused on the most suitable temperature for fresh- cut pumpkin with harmonization between benefits and drawbacks. Research method: Fresh-cut pumpkin cubes were pre–treated with 30% ethanol and 1% chitosan, then stored for 15 days at different temperatures (5 °C or T5, 10 °C or T10, 15°C or T15 and 25°C or T25). Findings: At refrigeration temperature (below 10 °C), fresh-cut pumpkin could maintain its overall visual quality until the end of storage duration (15 days). The difference in firmness and total carotenoid content between T5 and T10 suggested chilling injury occurrence when pumpkin was stored under 10°C. Besides, other nutritional parameters of these two treatments such as weight loss, total soluble solid content, total phenolic compounds and antioxidant capacity did not significantly differ from each other. Regardless of their nutritional composition, T15 and T25 became disqualified for consumption on day 3 and day 6, respectively due to dramatic shrinkage and microbial development. Limitations: Storage duration should be extended until all treatments reach unacceptable quality.The activity of cell wall degrading enzymes and antioxidant enzymes during storage should be investigated to support this study’s findings. Originality/Value: The preservation of fresh-cut pumpkin can be elevated to 10 °C to avoid chilling damage without altering much of their nutritional value.
Biotechnology and Tissue culture
Chadha Ayed; Chokri Bayoudh; Awatef Rhimi; Najla Mezghani; Faouzi Haouala; Bouthaina AL Mohandes Dridi
Abstract
Purpose: Tunisian garlic is widely threatened by the attack of several viruses genus. For this purpose, a reliable protocol was established for rapid in vitro propagation of local garlic (Allium sativum L.) cultivars for large-scale production of virus-free plants and high quality bulblets. ...
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Purpose: Tunisian garlic is widely threatened by the attack of several viruses genus. For this purpose, a reliable protocol was established for rapid in vitro propagation of local garlic (Allium sativum L.) cultivars for large-scale production of virus-free plants and high quality bulblets. Research method: Well disinfected shoot-tips of 1 mm were used as explants and cultivated on MS basal solid media enriched with various growth regulators: 6-Benzylaminopurine, α-Naphthaleneacetic acid, Kinetin, Indole-3-butyric acid and 2-isopentenyladenine for assessment of shoot formation, shoot proliferation and bulb formation. Findings: Among the different phytohormone concentrations and combinations, MS basal medium without any growth regulators (M0) was found optimal for shoot-tip initiation (96% explants development) and plantlets elongation (56.26 mm). For shoot proliferation, the M1 culture medium containing 1 mg L-1 BAP and 0.25 mg L-1 NAA was the best, giving a multiplication rate of 1.7 plantlets/explant. Shoots on M0 culture medium formed bulblets earlier. Multiple bulblets per explants were obtained on medium M22 containing 2 mg L-1 Kin and 0.1 mg L-1 NAA. Separated bulblets were transferred individually on bulbification media. Non-dividable bulblet was developed in various sizes. Research limitations: Bulblet acclimatization step needs to be well studied for high quality cloves production. Originality/value: This efficient optimized in vitro protocol will be successfully applied for large multiplication of virus-free garlic cultivars.
Plant Nutrition
Saleha Farjana; Md Ashraful Islam; Tamanna Haque
Abstract
Purpose: Cabbage is important fresh vegetable crop in most of the countries of the world. The experiment was conducted to evaluate the growth and yield of cabbage (Brassica oleracea var. capitata L. cv. Atlas-70) as influenced by organic and inorganic fertilizers, and mulching. Research methods: The ...
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Purpose: Cabbage is important fresh vegetable crop in most of the countries of the world. The experiment was conducted to evaluate the growth and yield of cabbage (Brassica oleracea var. capitata L. cv. Atlas-70) as influenced by organic and inorganic fertilizers, and mulching. Research methods: The experiment comprised of two different factors such as, factor-A; four different types of fertilizers viz. F0 (control, no fertilizer), F1 (vermicompost), F2 (inorganic fertilizer), and F3 (mixed of organic and inorganic fertilizer) and factor-B; types of mulches viz. M0 (control, no mulch), M1 (water hyacinth), M2 (rice straw), M3 (black polythene). This two factors experiment was conducted in Randomized Complete Block Design (RCBD) with three replications. Findings: Significant variation was found among the treatments. Result showed F3M3 (combination organic and inorganic fertilizer with black polythene) had the highest growth (plant height, stem length, root length, number of roots etc.) and yield (105.93 t/ha) in cabbage. The yield was 63.92% higher from the combined effect of fertilizer and mulch as black polythene (F3M3) compared to control (F0M0). So, mineral fertilizer and vermicompost with black polythene had the best performance considering the growth and yield of cabbage. Research limitations: Evaluation of different ratios of organic and inorganic fertilizers with mulching could have better outcome or findings of this work. Originality/Value: Combination of organic and inorganic fertilizers with black polythene as mulch have showed the highest growth and yield outcome of cabbage compared to other treatments combinations.This work has the opportunity to reduce the application of inorganic fertilizer to improve the soil health and environment in long run.
Postharvest Biology and Technology
Md. Saidee Rahman; Md. Kamrul Hassan; Fakhar Uddin Talukder; Md. Sohanur Rahman; Mst. Mishu Akther
Abstract
Purpose: An experiment was conducted to determine a suitable thickness of polypropylene bag as a package and appropriate storage temperature that will enhance an extended shelf life and quality of oyster mushroom. Research method: The experiment consisted of eight treatments under two factors (temperature ...
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Purpose: An experiment was conducted to determine a suitable thickness of polypropylene bag as a package and appropriate storage temperature that will enhance an extended shelf life and quality of oyster mushroom. Research method: The experiment consisted of eight treatments under two factors (temperature and thickness of polypropylene bag). Treatments: T1p 0= ambient temperature without wrapping (Control), T1p 1= ambient temperature & 50 µ PP bag, T1p 2= ambient temperature & 75 µ PP bag, T1p 3= ambient temperature & 100 µ PP bag, T2p 0= 3°C temperature without wrapping, T2p 1= 3°C temperature & 50 µ PP bag, T2p 2= 3°C temperature & 75 µ PP bag, T2p 3= 3°C temperature & 100 µ PP bag. The experiment was laid out in a Completely Randomized Design (CRD) with three replications. Findings: Highest moisture contents (90.80%) and shelf life (15 days) were observed in low temperature and wrapped in 75µ polypropylene bag (T2p 2). Highest dry matter content (82.92%) and highest weight loss (30.3%) were observed in ambient temperature and unwrapped condition (T1p 0) whereas lowest moisture contents (9.20%), lowest dry matter content (17.08%), lowest protein content(7.79%), and lowest shelf life (1.66) were observed in ambient temperature and unwrapped condition (T1p 0).Limitations: No limitations to report.Originality/Value: Combination of PP bags of proper thickness and low temperature can help in decreasing moisture loss and disease incidence which increase shelf life and maintain nutritional quality of oyster mushroom.
Postharvest Biology and Technology
Ramesh Faghihi; Kambiz Larijani; Vahid Abdossi; Pejman Moradi
Abstract
Purpose: Our objective was to undertake the green synthesis of silver nanoparticles using Grapefruit (Citrus paradise) peel extract and evaluate the effects of silver nanoparticles on Botrytis cinerea. Research method: The silver nanoparticles formation was evaluated at different temperatures and concentrations ...
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Purpose: Our objective was to undertake the green synthesis of silver nanoparticles using Grapefruit (Citrus paradise) peel extract and evaluate the effects of silver nanoparticles on Botrytis cinerea. Research method: The silver nanoparticles formation was evaluated at different temperatures and concentrations of AgNO3. The experiment was conducted during 2015 at Science and Research Branch, Islamic Azad University, Tehran, Iran. Main findings: Silver nanoparticles were successfully synthesized by Grapefruit's peel through a simple green and eco-friendly route. Aqueous extract of Grapefruit's peel was used synthesize nanosilver. The size of nanoparticle was determined at 5-65 nm, with SPR absorption at 420 nm in UV-Vis spectroscopy. Transmission electron microscopy (TEM) and X-ray diffraction spectroscopy (XRD) revealed that the synthesized nanoparticle was face centered. The silver nanoparticles characterized for their size and shape using scanning electron microscopy and TEM, respectively. XRD was used to determine the concentration of metal ions. Result indicated that nanosilver reduced the growth of Botrytis cinerea inviro culture. The highest antifungal effect was seen in the treatment with 40g/l nanosilver. In the other hand, the effect of nanosilver and time on diameter growth of Botrytis cinerea was not significant, individually (p≤1%). Limitations: No limitations were founded. Originality/Value: Green Synthesis of Nano is a reliable method for the nanoparticles synthesis and environmentally friendly approach.
Plant Propagation
Ha V. H. Nguyen; Duyen H. H. Nguyen
Abstract
Purpose: The aim of this study was to investigate the effects of nano-chitosan and chitosan coating on physico-chemical properties of strawberries during storage. Research methods: Fresh strawberries were coated with different concentrations of chitosan (1%, 1.5%. 2%) or nano-chitosan (0.2%, 0.4%, and ...
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Purpose: The aim of this study was to investigate the effects of nano-chitosan and chitosan coating on physico-chemical properties of strawberries during storage. Research methods: Fresh strawberries were coated with different concentrations of chitosan (1%, 1.5%. 2%) or nano-chitosan (0.2%, 0.4%, and 0.8%) and stored in 20C for 21 days. Findings: Coatingstrawberry with0.2% and 0.4% nano-chitosan preserved the overall quality index of the fruit up to 21 days. The treatments reduced weight loss, retained firmness, titratable acidity and L-ascorbic acid, significantly retarded malondialdehyde production and inhibited polyphenol oxidase activity of the stored fruit. The 0.2% nano-chitosan treatment reserved total soluble solid and total anthocyanin content better than the 0.4% nano-chitosan. Although 2% chitosan coating showed the positive effects, the overall quality index of the coated fruit was reduced below the acceptable level after 18 days, shorter as compared to the others coated with the lower concentrations of nano-chitosan. Research Limitations: Nano-chitosan, showing to be the effective coating material in this study, is not popular traded in the industry. Originality/Value: The combination of 0.2 % nano-chitosan coating and storing fresh strawberry at 2°C preserved the quality of fruits up to 21 days. The much lower concentrations of nano-chitosan showed higher positive effects as compared to the higher concentrations of chitosan. This would help to reduce the cost of postharvest handlings for the strawberry industry.
Medicinal Plants
Azim Ghasemnezhad; Madeh Ahmadi; Arezu Frouzy
Abstract
Purpose: Due the importance of taxol and based on the confirmed role of certain endophytes of Yew tree in the production of this vital compound, the present study was designed to investigate the effect of isolated endophytic fungi on vegetative and qualitative characteristics of stevia plant. Research ...
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Purpose: Due the importance of taxol and based on the confirmed role of certain endophytes of Yew tree in the production of this vital compound, the present study was designed to investigate the effect of isolated endophytic fungi on vegetative and qualitative characteristics of stevia plant. Research method: The experiment included five different endophytic fungi isolated from Yew tree. In order to do that, rooted cuttings of stevia were transferred to the pot after being inoculated with different isolated endophytic fungi (TB2, TB2-3, TB20, TB55) of Yew tree in four replications as a completely randomized design and were kept in outdoor conditions. To isolate endophytic fungi, root samples were collected from the yew trees in the Ziyarat forest located in Golestan province, Iran. Main findings: The results of the study revealed the positive effect of Yew tree endophytes as biotic agents on the growth and physiological parameters of stevia. Also, the fungal pathogenicity effect on stevia was not observed. In addition, the main stem diameter, internode spacing, leaf area and other measured parameters were affected by studied endophytes. Among of the five used isolates fungi, three were classified as stevia plant grows stimulator. Also, due to the highest total sugar and antioxidant activity of stevia, rest two isolates were classified as “bio-elicitor”. Limitations: There was no limitation to report. Originality/Value: The findings of the present study suggest that stevia, as a medicinal and economical herb, is capable of coexistence with yew endophytes. If taxol producer endophytes successfully transfer to stevia, it is possible to study the production of taxol in herbaceous plants, thereby opening the new door to easier and cheaper access to Taxol.
Plant Nutrition
Sally Wilkinson; Anna Weston; David Marks
Abstract
Purpose: Supplying plants with nitrogen in ammonium nitrate- or urea-based fertiliser is wasteful: much is degraded before acquisition, releasing environmental pollutants. Preventing urea degradation can reduce pollution and improve crop nitrogen use efficiency. We investigate benefits to ureic stabilisation, ...
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Purpose: Supplying plants with nitrogen in ammonium nitrate- or urea-based fertiliser is wasteful: much is degraded before acquisition, releasing environmental pollutants. Preventing urea degradation can reduce pollution and improve crop nitrogen use efficiency. We investigate benefits to ureic stabilisation, on flowering and stress tolerance, as organic nitrogen sources favourably alter biomass partitioning in this regard. Research Method: We test effects of adding chemically stabilised urea to soil, on the physical form and flowering of containerised, greenhouse-grown pelargonium, petunia, pansy and marigold, when transplanting seedlings to larger pots. Efficacies of stabilised urea, non-stabilised urea and industry standard fertiliser are compared under identical total nitrogen supply. The significance of treatment differences is calculated using a one-tailed t-test. Findings: Development is favourably altered by ureic stabilisation. Earliest changes measured are increased root lengths, leaf growth rates and chlorophyll concentrations. Plants then develop more shoots and 25-130% more flowers. Improvements arise partially through increased nitrogen longevity in soil, and partially through positive effects of urea itself on biomass partitioning between organs, and on plant physiology; giving rise to improved commercial attributes (more branches and flowers) and tolerance to stress (more root, less apical dominance, more chlorophyll). Research Limitations: Further research could measure leachate nitrogen content, and compare different methods of ureic stabilisation in more crops. Originality/Value: Urea stabilisation can increase fruit and flower yields, whilst reducing vulnerability to erratic climates, and fertiliser-derived pollution. We propose that urea’s effectiveness arises because plants have evolved strategies to proliferate whilst competing with micro-organisms for organic nitrogen.
Plant Stress
Rabeb El khaldi; Dhaouadi Latifa; Besser Houda
Abstract
Purpose: In Oasis areas, salinity becomes a real threat for sustainable agricultural production, and with the introduction of non-native varieties, salinity pressure is more expressed and has harmful outcomes to the local ecosystem and biodiversity. In this context the present study was conducted ...
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Purpose: In Oasis areas, salinity becomes a real threat for sustainable agricultural production, and with the introduction of non-native varieties, salinity pressure is more expressed and has harmful outcomes to the local ecosystem and biodiversity. In this context the present study was conducted to compare salt stress behavior of local oasian and introduced cultivars. Research method: Seeds of two local oasian cultivars namely Djerid (Dj) and Nefta (Ne) and one exotic cultivar namely Cayenne (Ca) were treated with four salt concentrations (0; 2.5; 5 and 7g/l NaCl). Salt stress responses were evaluated using germination parameters and early seedling growth. Findings: Results revealed that the increase of salinity level had negatively effect on germination and early seedling for the three cultivars. However oasian cultivars were found to be the most tolerant to salinity. Ne cultivar showed the highest germination percentage and germination index and the lowest mean germination time at 7g/l NaCl concentration. Also, “Ne” performs better on seedling traits as indicated by the less effect of the high salinity on radicle and plumule length as well as plumule fresh weight, compared to the other cultivars. The introduced cultivar “Ca” was identified to be the most sensitive to salt stress. Limitations: No limitations were founded. Originality/Value: This study valorized the autochthone chili pepper cultivars by highlighting their salt stress tolerance. Thus, oasian genotypes could be very useful as genetic resources for the development of chili pepper cultivars with improved germination and seedling growth under salt-stress conditions.
Fresh-Cut Produce
Farid Moradinezhad
Abstract
Purpose: Mango is an invaluable and climacteric fruit with a short shelf life. Minimally fresh‐cut mango fruit market is growing very fast, particularly in Europe and the United States, and recently in Asian countries and the Middle East. Consumers demand high quality in fresh-cut fruit. Research method: ...
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Purpose: Mango is an invaluable and climacteric fruit with a short shelf life. Minimally fresh‐cut mango fruit market is growing very fast, particularly in Europe and the United States, and recently in Asian countries and the Middle East. Consumers demand high quality in fresh-cut fruit. Research method: Effect of different post-cutting chemical treatments including calcium chloride (CaCl2), ascorbic acid (AA), salicylic acid (SA), oxalic acid (OA) and citric acid (CA) on the quality maintenance and extending the shelf life of fresh-cut were evaluated. Treated slices were placed into the Polypropylene (PP) containers with lids (Passive MAP) and stored at 4±1 °C for two weeks. Findings: The results showed that the highest firmness (7.01 N) was recorded in CaCl2 treated fresh-cut mangoes at the end of storage. The TSS significantly was lower in all treated fresh-cuts compared to the control. Besides, treated mango slices with AA, SA, and OA had a higher antioxidant activity than other treatments and control. Interestingly, mango slices that were treated with CaCl2 had a higher texture (4.33) value compared to the control and other treatments. The AA and CaCl2 treatments maintained the fresh-cut taste compared to the control and other chemical treatments after 14 days of cold storage. The highest shelf life was observed in CaCl2 (17.5 days) and SA (17.0 days) treatments. The hue values were best maintained in OA and CA treatments. Moreover, the total aerobic mesophilic bacterial and fungal counts remained below detection limits at the end of storage. Limitations: No limitations were found to report. Originality/Value: In conclusion, CaCl2, AA and SA were better treatments for fresh-cut mangoes because higher acceptability and sensorial quality and also longer shelf life.
Greenhouse Production
Nazila Khanbabaloo; Hanifeh Seyed hajizadeh; Farhad Behtash
Abstract
Todays, contamination of agricultural soils by different ways of salinity is increasing as a result of human and environmental factors that would reduce yield and quality of agricultural crops. However, there are some plants that can tolerate some degrees of salinity. Tomato is one of the horticultural ...
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Todays, contamination of agricultural soils by different ways of salinity is increasing as a result of human and environmental factors that would reduce yield and quality of agricultural crops. However, there are some plants that can tolerate some degrees of salinity. Tomato is one of the horticultural crops, moderately sensitive to salinity even that salinity can improve its quality. So, a study was conducted using four varieties of tomato under normal and salt stress (3, 6 and 9 dS m-1) hydroponic conditions to evaluate tomato quality. The results showed that increasing in salinity improves the quality and taste of tomato with a reduction in pericarp thickness, fresh weight and total protein. However, when the degree of salinity exceeds 6 dS m-1, a significant difference was observed in fruit yield which was variety dependent. Also, there is a significant difference between salt tolerance, performance, and taste of different varieties. Furthermore, it is found that the activity of guaiacol peroxidase and catalase enzymes increased as well as an increase in the level of salinity. Tomato cv. Super Chief with the highest number of fruits per plant, high level of proline as well as catalase activity, has the best performance against salinity levels of soil and was more resistant to salinity than other varieties. Also, tomato ‘Super Chief’ had the highest taste index with a slightly decline in the yield. Salt stress tomatoes have more carotenoid than controls but the level of 9 dS m-1 salinity was not tolerable by all varieties.
Postharvest Biology and Technology
Farid Moradinezhad; Mehdi Khayyat; Fatemeh Ranjbari; Zahra Maraki
Abstract
Purpose: Pomegranate fruit is highly susceptible to water loss and chilling injuries during postharvest cold storage. Thus an experiment was conducted to evaluate the effects of modified atmosphere packaging on pomegranate shelf life and chilling resistance under cold storage. Research method: Treatments ...
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Purpose: Pomegranate fruit is highly susceptible to water loss and chilling injuries during postharvest cold storage. Thus an experiment was conducted to evaluate the effects of modified atmosphere packaging on pomegranate shelf life and chilling resistance under cold storage. Research method: Treatments were vacuum packed and unpacked fruits (control) that were stored in a cold room at 5 ± 0.5 °C and 80 ± 5% RH. At the end of each storage period (30, 40, 50, 70, 90 and 130 days), packed fruits were assessed for physicochemical and quality parameters while control fruit were assessed after 90 days of storage. Findings: Results showed that packaging improves TSS/TA, and some color attributes, and decreased anthocyanin, total acidity, weight loss, electrolyte leakage, chilling injury index and unmarketable fruits. Evaluation of traits in different durations showed that vacuum packaging significantly reduced weight loss, electrolyte leakage, chilling injury index, and interestingly decreased the number of unmarketable fruits by 30%, compared with control. Anthocyanin contents significantly decreased since day 70 of storage. The a* and chroma values of peel significantly increased in packaging treatments as storage period progressed, however, hue value significantly decreased, compared with control. Research limitations: There was no significant limitation to report. Originality/Value: The results suggest that bulk vacuum packaging can be used for up to 3 months to successfully alleviate chilling injury, reduce weight loss and the postharvest losses, and to extend the storage life of pomegranate fruit during cold storage.
Plant Growth Regulators
Andreas Kleiber; Margaret Sedgley; Nancy Bagnato; Farid Moradinezhad
Abstract
Purpose: The study of effects of 1-Methylcyclopropene (1-MCP) on ripening of bananas is still an important issue for commercial application of 1-MCP on bananas. Research Method: Mature green bananas were treated with ethylene only (100 µL L-1 for two consecutive days) and ethylene the same treatment ...
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Purpose: The study of effects of 1-Methylcyclopropene (1-MCP) on ripening of bananas is still an important issue for commercial application of 1-MCP on bananas. Research Method: Mature green bananas were treated with ethylene only (100 µL L-1 for two consecutive days) and ethylene the same treatment followed by 1-MCP (300 nL L-1) for 24 h to evaluate the ethylene and 1-MCP effects on ethylene biosynthesis enzyme activities. Ethylene production of whole banana fruit, ACC synthase (ACS) and ACC oxidase (ACO) activities in the pulp and peel of samples were measured. Findings: The result showed that ethylene production rate by the control fruit was significantly greater than the ethylene production rate by the 1-MCP-treated fruit at days 4, 8 and 10. However, changes in ethylene production were similar in both control and 1-MCP-treated bananas. The banana peel and pulp show different patterns of ethylene production during ripening. At the onset of ripening pulp tissues showed higher levels of ACS, and lower levels of ACO activity than peel. Assays of ACO and ACS activities in ethylene-treated fruit showed that the peel had higher levels of ACO activity than the pulp. The ACO and ethylene production were inhibited by 1-MCP treatment whereas ACS increased following 1-MCP application. Research limitations: Evaluation of ACS and ACO activities during different seasons. Originality/value: Pulp and the peel of bananas respond differently to ethylene and 1-MCP treatment with a greater impact on peel than the pulp. The findings of this study allow 1-MCP to be used in a more commercially reliable manner.
Olericulture
Gebremedhin Hailay; Awgchew Haymanot
Abstract
Purpose: Swiss chard (Beta vulgaris L.) is highly appreciated for its nutritional properties, year round availability, low cost and wide use. In central highlands of Ethiopia, Swiss chard has been producing in urban and peri-urban areas but limited information is available about its nutrition and planting ...
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Purpose: Swiss chard (Beta vulgaris L.) is highly appreciated for its nutritional properties, year round availability, low cost and wide use. In central highlands of Ethiopia, Swiss chard has been producing in urban and peri-urban areas but limited information is available about its nutrition and planting distance. Research method: An experiment was designed to study the effects of 50, 100 and 150 kg ha-1 and control (0 kg ha-1), and different spacing with row including 20 cm, 30 cm and 40 cm distance on growth, yield and nutritional composition of Swiss chard in Debre Berhan. Treatments were set in randomized complete block design with three replications making 45 treatment combinations. Findings: Results indicated that total plant fresh weight, total plant dry weight, above ground fresh weight, above ground dry weight, root fresh weight, root dry weight, plant height, leaf number per plant were significantly affect by the interaction effect of plant spacing and N levels. Leaf area index was significantly affected by intra-row spacing but not by N rate and their interaction. Remarkable difference was recorded on total nitrogen, nitrate contents and vitamin C of leaves due to varied N levels. Generally, growth attributes, yield performance and nutritional composition of Swiss chard obtained best at the combination of 100 kg N ha-1 N and 40 cm plant spacing. Research limitation: Intra-Row needs further study above the 40 cm planting distance. Originality/Value: combination of 100 kg N ha-1 and 40 cm in-row spacing can be used for Swiss chard production in Debre Berhan central highlands of Ethiopia.
Food Science and Technology
Olusola Michael Obembe; Durojaye Olanrewaju Ojo; Kayode David Ileke
Abstract
Purpose: The leaves of Telfairia occidentalis is affected by the various methods of drying and storage employed after harvest. This research work is therefore designed to determining the best drying method for the preservation of Telfairia occidentalis using the mineral and proximate constituents as ...
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Purpose: The leaves of Telfairia occidentalis is affected by the various methods of drying and storage employed after harvest. This research work is therefore designed to determining the best drying method for the preservation of Telfairia occidentalis using the mineral and proximate constituents as indices. Research method: The cleaned, fresh leaves were then divided into three portions. The first portion was sundried, the second portion was air-dried and the third portion was oven-dried. Each sample was analyzed for proximate composition and mineral content. Findings: The results of the various analysis generally showed that the oven-dried samples have the highest mineral content followed by sun-dried and then the air-dried. The ash content was most top (12.78%) in the oven-dried example, sample followed by air-dried, sun-dried, and then fresh samples while the moisture content was most top (82.62%) in the clean examplefollowed by sun-dried, air-dried and oven-dried with 11.40, 9.18%, and 6.33% moisture respectively. The carbohydrate content was most top (32.62%) in the sun-dried sample followed by air-dried, oven-dried and then fresh ones with 30.10, 29.58, and 4.44% respectively. The protein content was highest (32.69%) in the oven-dried samplefollowed by air-dried, clean, and then sun-dried examples with 30.16, 5.92, and 2.70%, respectively. The fat content was highest (7.50%) in the air-dried and oven-dried examples followed by sun-dried, and the fresh ones with 6.85 and 1.85%, respectively. The fiber content was most top (11.16%) in the oven-dried example followed by air-dried, sun-dried, and the fresh ones with 10.69, 9.16, and 2.94% respectively. Limitations: The proximate and mineral compositions of the leaves were investigated after drying and storage. Originality/Value: The results showed that the oven-dried samples in most cases, have the highest proximate and mineral composition. So, oven-drying is the most nutritionally viable method in the preservation and storage of Telfairia occidentalis.
Postharvest Biology and Technology
Mahdei Behshti; Mehdi Jahani; Mohmmad Hossein Aminifard; Seyyedeh Atefeh Hosseini
Abstract
Purpose: The effect of abusing chemical biocides in controlling pests and diseases has drawn the attention of policymakers to the development of methods potentially available in nature for this purpose. Research method: In the present study, the inhibitory effects of four different essential oils against ...
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Purpose: The effect of abusing chemical biocides in controlling pests and diseases has drawn the attention of policymakers to the development of methods potentially available in nature for this purpose. Research method: In the present study, the inhibitory effects of four different essential oils against Botrytis cinerea were tested at various concentrations (0, 200, 400, 600, and 800 μL L-1) in in vitro and in vivo. Main findings: The in vitro results showed that the growth of B. cinerea was completely inhibited by the application of anise oil at concentrations of 800 μL L-1. The in vivo results indicated that treated fruits with marjoram oil had more total soluble solids, and anthocyanin content in comparison to anise, chamomile, and black caraway oil. Furthermore, among essential oils, treated fruits with black caraway essential oils had the lowest pH, while anise, chamomile, and marjoram oil had the highest pH. The highest anthocyanin content and pH were obtained at 200 and 400 μL L-1 concentration and lowest values were found at a control treatment (respectively). The most total soluble solids were observed at control treatment and the lowest values were recorded at 600 μL L-1. The application of each essential oil decreased the percentage loss in fresh weight significantly and increased the storage-life of the fruit. Limitations: Higher cost of application was a limitation. Originality/Value: This research confirms the antifungal effects of anise, fennel, chamomile, and marjoram essential oils both in vitro and in vivo on grape fruits postharvest. Therefore, these essential oils could be an alternative to chemicals to control postharvest phytopathogenic fungi on grape fruits.
Plant Breeding and Biotechnology
Shahin Jahangirzadeh; Reza Azadi Gonbad; Koorosh Falakro
Abstract
Purpose: The tea plant is one of the most important products in the northern region of Iran, and plays an essential role in the region's economy. Since today many tea plants in the region are being destroyed for various reasons, so having information about the genetics of those trees helps design breeding ...
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Purpose: The tea plant is one of the most important products in the northern region of Iran, and plays an essential role in the region's economy. Since today many tea plants in the region are being destroyed for various reasons, so having information about the genetics of those trees helps design breeding programs to reach appropriate plants for specific purposes. Research Method: SRAP markers, using eight primer combinations, were used to study the genetic relationships of 27 tea plant samples. Findings: In total, these eight combinations produced 41 scorable bands, 70.63% of which were polymorphic. The calculated PIC for all combinations was from 0.23 to 0.43 at an average of 0.36. Data analysis was performed by NTSYS software using Jaccard's similarity coefficient to determine the amount of similarity and the dendrogram was drawn based on UPGMA. Based on molecular data, the range of similarity between samples varied from 0.393 to 0.933. Samples were divided into five groups at a similarity level of 0.65. The fifth group (E) was divided into four subgroups at a similarity level of 0.75. Research limitations: Application of another marker system such as SSR and AFLP can help to understand the relationships of samples better. Originality/Value: In general, the study of genetic diversity showed that the SRAP marker could be useful in identifying polymorphic regions and estimating genetic distances and germplasm management in tea plants.
Biotechnology and Tissue culture
Shahin Jahangirzadeh Khiavi; Koorosh Falakro; Sanam Safaei Chaeikar
Abstract
Purpose: A significant number of genetic resources of Camellia sinensis and its allied genotypes have been collected and preserved in Iran TRC. Information about them is mostly based on morphological data. Research method: PCR-RFLP technique and morphological characters were used for the identification ...
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Purpose: A significant number of genetic resources of Camellia sinensis and its allied genotypes have been collected and preserved in Iran TRC. Information about them is mostly based on morphological data. Research method: PCR-RFLP technique and morphological characters were used for the identification of organelle DNA (cpDNA) diversity in 25 tea genotypes. Twenty-one qualitative and quantitative characteristics were evaluated. Findings: A pair-wise similarity among the samples ranged from 0.14 to 0.66 based on morphological data. The dendrogram was designed, and samples were grouped into three main clusters at 0.38 similarity. Using three universal primer pairs which introduced for chloroplast amplified about 4070bp of cpDNA, following the digestion of fragments with three restriction endonucleases (HinfI, AluI and PstI) and the result of this method was introduced six haplotypes. The most significant and widespread haplotype was H2 (frequency ≈ 28%). All of the detected mutations were insertion-deletions and they ranged from 30 to 60 bp. The calculated total cpDNA diversity in populations (hT), a major portion of it was within populations were (hS) and genetic differentiation among populations (GST) were 0.43, 0.17 and 0.61, respectively. It should have been noted that the calculated GST was low and no structure could be identified. Limitations: Applying allied species and using more potent markers such as cpSSR and sequencing can lead to more accurate results. Originality/Value: The results of this study indicate that the PCR-RFLP method and morphological characters are applicable in the identification of tea genotypes and cultivars. In studying Camellia genus phylogeny, the polymorphism in cpDNA has to be considered carefully.
Fresh-Cut Produce
Ben Amara Mouna; Amodio Maria Luisa; Colelli Giancarlo; Ben Abda Jameleddine
Abstract
Purpose: Ready to eat arils can be a value-added product as an alternative use for the whole pomegranate fruit by offering more convenience to the consumer. Recently, the diffusion of local cultivars with typical and unique quality characteristics will offer new opportunities for the fresh international ...
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Purpose: Ready to eat arils can be a value-added product as an alternative use for the whole pomegranate fruit by offering more convenience to the consumer. Recently, the diffusion of local cultivars with typical and unique quality characteristics will offer new opportunities for the fresh international market. Research method: This study aimed to evaluate the quality of arils from five cultivars, namely Gabsi (GB), Jebali (JB), Khalledi (KH), Tounsi (TN), and Zehri (ZH) to be used for ready to eat market, as well as to provide a form of valorization for these cultivars. Findings: Significant differences found between cultivars for most of the evaluated quality parameters. KH, GB, and JB were the cultivars with the best initial quality. PCA separated the investigated cultivars based on the storage period. Among the studied cultivars, the main changes in color and sensory quality attributes during storage have been registered for the cultivars JB and KH. TN showed slight color difference during storage. GB had right color intensity, maintained high content of anthocyanins, and the best sensory evaluation at the end of storage. Limitations: Based on their nutritional quality, cultivars GB and KH were the best cultivars for ready to eat arils processing. However, a proper selection of initial quality should be considered. Originality/Value: The richness of local Tunisian pomegranate cultivars with its typical and unique quality traits. They could be used as a ready to eat form to valorize the whole fruit thereby, will enhance marketing demand.
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